Retrospective Study
Copyright ©The Author(s) 2021.
World J Gastrointest Oncol. Jul 15, 2021; 13(7): 716-731
Published online Jul 15, 2021. doi: 10.4251/wjgo.v13.i7.716
Table 1 Patient baseline and correlation between protein phosphatase 2 regulatory subunit B''α expression and clinicopathologic characteristics in hepatocellular carcinoma
Clinicopathological variable
Patients, n (%)
PPP2R3A expression levels
Statistical results
< 4
≥ 4
Gender
Male105 (97.2)79 (73.1)26 (24.1)P = 0.195; X2 = 1.679
Female3 (2.8)3 (2.8)0 (0)
Age (yr)
≤ 5045 (41.7)37 (34.2)8 (7.4)P = 0.255
> 5063 (58.3)45 (41.7)18 (16.7)X2 = 1.673
Preoperative treatment
No59 (54.6)47 (43.5)12 (11.1)P = 0.370
Yes49 (45.4)35 (32.4)14 (13)X2 = 0.993
AFP (ng/mL)
< 1324 (22.2)21 (19.4)3 (2.8)P = 0.0031; X2 = 11.513
13-100040 (37.0)35 (32.4)5 (4.6)
> 100044 (40.8)26 (24.1)18 (16.7)
HBeAg
Negative72 (66.7)56 (51.9)16 (14.8)P = 0.634
Positive36 (33.3)26 (24.1)10 (9.2)X2 = 0. 405
HBV-DNA (copy/mL)
≤ 175 (69.4)57 (52.8)18 (16.7)P = 1.000
> 133 (30.6)25 (23.1)8 (7.4)X2 = 0.001
MELD
< 1585 (78.7)65 (60.2)20 (18.5)P = 1.000
≥ 1523 (21.3)17 (15.7)6 (5.6)X2 = 0.065
Child-Pugh
A65 (60.2)52 (48.3)13 (12.0)P = 0.424; X2 = 3.952
B27 (25.0)17 (15.7)10 (9.2)
C16 (14.8)13 (12.0)3 (2.8)
Pathology
Well differentiation5 (4.6)5 (4.6)0 (0) P = 0.296
Moderate differentiation98 (90.8)74 (68.5)24 (22.2)X2 = 3.382
Poor differentiation5 (4.6)3 (2.8)2 (1.9)
TNM-t
T146 (42.6)38 (35.2)8 (7.4)P = 0.0001; X2 = 16.601
T221 (19.4)21 (19.4)0 (0)
T341 (38.0)23 (21.3)18 (16.7)
Length (cm)
≤ 331 (28.7)24 (22.2)7 (6.5)P = 0.198; X2 = 3.294
4-538 (35.2)32 (29.6)6 (5.6)
≥ 639 (36.1)26 (24.1)13 (12.0)
Tumor number
136 (33.3)30 (27.8)6 (5.6)P = 0.240
≥ 272 (66.7)52 (48.1)20 (18.5)X2 = 1.621
Microsatellite stove
No55 (50.9)44 (40.7)11 (10.2)P = 0.371
Yes53 (49.1)38 (35.2)15 (13.9)X2 = 1.018
Envelope invasion
No75 (69.4)57 (52.8)18 (16.7)P = 0.0011
Yes33 (30.6)25 (23.1)8 (7.4)X2 = 1.000
Vascular invasion
No69 (63.9)53 (49.1)16 (14.8)P = 0.817
Yes39 (36.1)29 (26.9)10 (9.2)X2 = 0.082
Lymph node metastasis
No92 (85.2)72 (66.7)20 (18.5)P = 0.207
Yes16 (14.8)10 (9.2)6 (5.6)X2 = 1.707
Liver metastasis
No67 (62.0)55 (50.9)12 (11.1)P = 0.066
Yes41 (38.0)27 (25)14 (13)X2 = 3.668
Chest metastasis
No75 (69.4)61 (56.5)14 (13)P = 0.055
Yes33 (30.6)21 (19.4)12 (11.1)X2 = 3.927
Hepatitis B
No recurrence102 (94.4)78 (72.9)24 (22.4)P = 0.427
Recurrence5 (4.6)3 (2.8)2 (1.9)X2 = 0.632
Table 2 Univariate analysis results of overall survival and tumor-free survival risk factors and patient characteristics
Variables
OS
RFS
Univariate
Univariate
P value
P value
PPP2R3A (< 4 vs ≥ 4) 0.00010.0251
Age (≤ 50 vs > 50)0.1220.213
Child-Pugh (≤ 6 vs 7-9 vs ≥ 10) 0.3750.206
MELD (< 15 vs ≥ 15)0.8270.809
HBeAg (Negative vs Positive)0.4610.120
HBV-DNA (Negative vs Positive)0.5810.090
Pathology (Well vs Moderate vs Poor)0.0471 < 0.051
Tumor number (1 vs > 1)0.00010.0001
Tumor size (cm) (≤ 3 vs 4-5 vs ≥ 6)0.0051 < 0.051
TNM-t (1 vs 2 vs 3)0.0001 < 0.051
Microsatellite stove (No vs Yes)0.00010.0001
Vascular invasion (No vs Yes) 0.0110.0001
Lymph node metastasis (No vs Yes)0.6710.885
Capsule invasion (No vs Yes)0.1020.0051
Liver metastasis (No vs Yes)0.1240.0011
Chest metastasis (No vs Yes)0.00110.0001
AFP (ng/mL) (< 13 vs 13-1000 vs > 1000)0.00010.0001
Preoperative treatment (No vs Yes)0.6850.604
Table 3 COX multivariate regression analysis results of overall survival in hepatocellular carcinoma patients after liver transplantation
Variables
B
SE
Wald
P value
OR
95%CI for Exp (B)
Lower
Upper
Tumor size (≤ 3 cm vs ≥ 6 cm)1.0150.3807.1200.00812.7601.3095.816
Tumor number (1 vs ≥ 2)1.5490.41513.9460.00014.7072.08810.612
PPP2R3A (< 4 vs ≥ 4)1.0650.3678.3970.00412.9001.4115.960
Table 4 COX multivariate regression analysis results of tumor recurrence after liver transplantation in hepatocellular carcinoma patients
Variables
B
SE
Wald
P value
OR
95%CI for Exp (B)
Lower
Upper
capsule invasion0.6330.2854.9410.02611.8831.0783.290
AFP (ng/mL) (< 13 vs > 1000)1.1450.3769.2630.00213.1431.5036.571
Tumor number (1 vs > 1)1.3400.35414.3580.00013.8191.9107.638
PPP2R3A14.5620.0241
PPP2R3A (6 vs 0) -1.5270.4899.7750.00210.2170.0830.566
PPP2R3A (6 vs 2) -1.7730.51112.0430.00110.1700.0620.462
PPP2R3A (6 vs 3) -1.117.5154.7140.03010.3270.1190.897
PPP2R3A (6 vs 4)-1.1410.4875.4890.01910.3200.1230.830
PPP2R3A (6 vs 5)-0.6300.6970.8170.3660.5320.1362.088