Analysis of the potential biological value of pyruvate dehydrogenase E1 subunit β in human cancer

Figure 14  Effects of siRNA-pyruvate dehydrogenase E1 subunit β on proliferation, migration and invasion of Huh7 hepatoma cells. A: Transfection efficiency of siRNA-pyruvate dehydrogenase E1 subunit β (PDHB) in Huh7 cell lines; B: The relative expression of mRNA reflects the efficiency of siRNA-PDHB transfection; C: siRNA-PDHB transfection efficiency by protein expression level; D: The CCK-8 method detected the proliferation capacity of Huh7 cell lines; E: Colony formation assay to determine the proliferation capacity of Huh7 cell lines; F: The histogram shows the number of colonies formed; G: Cell scratch assay to detect the migration capacity of Huh7 cell lines; H: Histogram of quantification of cell scratch assay results; I: Transwell method was used to detect the migration and invasion capacity of Huh7 cell lines; J: The histogram shows the number of migrating cells; K: The histogram shows the number of invading cells. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. PDHB: Pyruvate dehydrogenase E1 subunit β.