Basic Study
Copyright ©The Author(s) 2019.
World J Gastrointest Oncol. Mar 15, 2019; 11(3): 195-207
Published online Mar 15, 2019. doi: 10.4251/wjgo.v11.i3.195
Figure 2
Figure 2 The restricted VDJ patterns and distribution of immunoglobulin heavy chain in cancer and normal cells. A: V-J-CDR3 map of immunoglobulin heavy chain (IgH) expressed in normal B cells[26], colon cancer cells and normal epithelial cells. Each rectangle represents a unique V-J-CDR3 nucleotide sequence and the size denotes its relative frequency. Colors for each rectangle are chosen randomly and, thus, do not match between plots; B: The distribution of VHs expressed in cancer and normal epithelial cells; C: The utilizations of VHs in cancer and normal epithelial cells. The order of VHs on the X-axis corresponds to its position on a chromosome; D: Utilizations of 7 VH and 6 JH families in cancer and normal cells from patients with colon cancer (red and blue columns), and B cells from peripheral blood of a healthy donor[28] (orange columns). Small horizontal lines indicate the mean ± SEM. All data comparing cancer with normal cells were determined by the two-tailed unpaired Student’s t-test and none of the differences were significant (P > 0.05). The percentage of VH and JH families in B cells (D) was derived from the data from other sources[28]; thus, statistical analysis was not performed.