Experience with peritoneal mesothelioma at the Milan National Cancer Institute

Figure 1 Time courses of the mean concentrations of doxorubicin and cisplatin in perfusate and plasma. Solid lines obtained by nonlinear least squares regression analyses of data. Open symbols: Doxorubicin (DXR); Filled symbols: Cisplatin (CDDP).

Figure 2 Representative Western blotting experiments illustrating survivin expression in STO cells exposed to oligofectamine alone or transfected with control siRNA and survivin siRNA. A: β-actin was used as a control for protein loading; B: Densitometric quantification of survivin band intensities in oligofectamine-exposed cells (empty column) and cells transfected with the control siRNA (gray column) or the survivin siRNA (black column). Data represent mean ± SD of 3 independent experiments. ^aP < 0.02; ^bP < 0.01; Student’s t test; such inhibition was highest (around 80%; P < 0.01) at 48 h and 72 h after transfection and still appreciable, although to a lesser extent (around 50%; P < 0.02), at 96 h.

Figure 3 Effects of siRNA-mediated survivin down-regulation on the apoptotic response of STO cells to cisplatin and doxorubicin. The percentage of cells with an apoptotic morphology with respect to the overall population as assessed by fluorescence microscopy in STO cells exposed to oligofectamine alone (empty column) and transfected with control siRNA (gray column) or survivin siRNA (black column) in the absence or presence of different cisplatin or doxorubicin concentrations. Data represent mean ± SD of 3 independent experiments. ^aP < 0.05; ^bP < 0.01; ^dP < 0.001; Student’s t test.