1
|
Meira DI, Proença M, Rebelo R, Barbosa AI, Rodrigues MS, Borges J, Vaz F, Reis RL, Correlo VM. Chitosan Micro-Membranes with Integrated Gold Nanoparticles as an LSPR-Based Sensing Platform. BIOSENSORS 2022; 12:bios12110951. [PMID: 36354460 PMCID: PMC9687842 DOI: 10.3390/bios12110951] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/27/2022] [Revised: 10/19/2022] [Accepted: 10/27/2022] [Indexed: 05/05/2023]
Abstract
Currently, there is an increasing need to develop highly sensitive plasmonic sensors able to provide good biocompatibility, flexibility, and optical stability to detect low levels of analytes in biological media. In this study, gold nanoparticles (Au NPs) were dispersed into chitosan membranes by spin coating. It has been demonstrated that these membranes are particularly stable and can be successfully employed as versatile plasmonic platforms for molecular sensing. The optical response of the chitosan/Au NPs interfaces and their capability to sense the medium's refractive index (RI) changes, either in a liquid or gas media, were investigated by high-resolution localized surface plasmon resonance (HR-LSPR) spectroscopy, as a proof of concept for biosensing applications. The results revealed that the lowest polymer concentration (chitosan (0.5%)/Au-NPs membrane) presented the most suitable plasmonic response. An LSPR band redshift was observed as the RI of the surrounding media was incremented, resulting in a sensitivity value of 28 ± 1 nm/RIU. Furthermore, the plasmonic membrane showed an outstanding performance when tested in gaseous atmospheres, being capable of distinguishing inert gases with only a 10-5 RI unit difference. The potential of chitosan/Au-NPs membranes was confirmed for application in LSPR-based sensing applications, despite the fact that further materials optimization should be performed to enhance sensitivity.
Collapse
Affiliation(s)
- Diana I. Meira
- Physics Center of Minho and Porto Universities (CF-UM-UP), Campus de Azurém, University of Minho, 4800-058 Guimarães, Portugal
- 3 B’s Research Group, I3Bs—Research Institute on Biomaterials, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Biodegradables and Biomimetics of University of Minho, AvePark—Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal
| | - Manuela Proença
- Physics Center of Minho and Porto Universities (CF-UM-UP), Campus de Azurém, University of Minho, 4800-058 Guimarães, Portugal
| | - Rita Rebelo
- 3 B’s Research Group, I3Bs—Research Institute on Biomaterials, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Biodegradables and Biomimetics of University of Minho, AvePark—Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal
- ICVS/3B’s—PT Government Associated Laboratory, AvePark, Zona Industrial da Gandra S. Claudio do Barco, Caldas das Taipas, 4806-909 Guimarães, Portugal
- Correspondence: (R.R.); (J.B.); Tel.: +351-253510900 (R.R.); +351-253510471 (J.B.)
| | - Ana I. Barbosa
- 3 B’s Research Group, I3Bs—Research Institute on Biomaterials, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Biodegradables and Biomimetics of University of Minho, AvePark—Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal
- ICVS/3B’s—PT Government Associated Laboratory, AvePark, Zona Industrial da Gandra S. Claudio do Barco, Caldas das Taipas, 4806-909 Guimarães, Portugal
| | - Marco S. Rodrigues
- Physics Center of Minho and Porto Universities (CF-UM-UP), Campus de Azurém, University of Minho, 4800-058 Guimarães, Portugal
| | - Joel Borges
- Physics Center of Minho and Porto Universities (CF-UM-UP), Campus de Azurém, University of Minho, 4800-058 Guimarães, Portugal
- LaPMET—Laboratory of Physics for Materials and Emergent Technologies, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
- Correspondence: (R.R.); (J.B.); Tel.: +351-253510900 (R.R.); +351-253510471 (J.B.)
| | - Filipe Vaz
- Physics Center of Minho and Porto Universities (CF-UM-UP), Campus de Azurém, University of Minho, 4800-058 Guimarães, Portugal
- LaPMET—Laboratory of Physics for Materials and Emergent Technologies, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
| | - Rui L. Reis
- 3 B’s Research Group, I3Bs—Research Institute on Biomaterials, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Biodegradables and Biomimetics of University of Minho, AvePark—Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal
- ICVS/3B’s—PT Government Associated Laboratory, AvePark, Zona Industrial da Gandra S. Claudio do Barco, Caldas das Taipas, 4806-909 Guimarães, Portugal
| | - Vitor M. Correlo
- 3 B’s Research Group, I3Bs—Research Institute on Biomaterials, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Biodegradables and Biomimetics of University of Minho, AvePark—Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal
- ICVS/3B’s—PT Government Associated Laboratory, AvePark, Zona Industrial da Gandra S. Claudio do Barco, Caldas das Taipas, 4806-909 Guimarães, Portugal
| |
Collapse
|
2
|
Lepore M, Delfino I. Optical Sensors Technology and Applications. SENSORS (BASEL, SWITZERLAND) 2022; 22:7905. [PMID: 36298255 PMCID: PMC9611779 DOI: 10.3390/s22207905] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/22/2022] [Accepted: 09/26/2022] [Indexed: 06/16/2023]
Abstract
Optical methods are non-invasive tools, and their use in various fields, including sensing applications, is continuously increasing, which is thanks to the continuous development of innovative low-cost sources and detectors [...].
Collapse
Affiliation(s)
- Maria Lepore
- Dipartimento di Medicina Sperimentale, Università della Campania “Luigi Vanvitelli”, I-80138 Napoli, Italy
| | - Ines Delfino
- Dipartimento di Scienze Ecologiche e Biologiche, Università della Tuscia, I-01100 Viterbo, Italy
| |
Collapse
|