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Mostafa-He G, Alanazi M, Abdelmawll H. Antioxidant, Anti-Inflammatory and Antiapoptotic Effect of Mirtazapine Mitigates Cyclophosphamide-Induced Testicular Toxicity in Rats. INT J PHARMACOL 2023. [DOI: 10.3923/ijp.2023.166.177] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/19/2023]
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Ghosh A, Sarmah P, Patel H, Mukerjee N, Mishra R, Alkahtani S, Varma RS, Baishya D. Nonlinear molecular dynamics of quercetin in Gynocardia odorata and Diospyros malabarica fruits: Its mechanistic role in hepatoprotection. PLoS One 2022; 17:e0263917. [PMID: 35313329 PMCID: PMC8936497 DOI: 10.1371/journal.pone.0263917] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/14/2021] [Accepted: 01/31/2022] [Indexed: 12/28/2022] Open
Abstract
Liver performs number of critical physiological functions in human system. Intoxication of liver leads to accumulation of free radicals that eventually cause damage, fibrosis, cirrhosis and cancer. Carbon tetrachloride (CCl4) belongs to hepatotoxin is converted to a highly reactive free radical by cytochrome P450 enzymes that causes liver damage. Plant extracts derived quercetin has substantial role in hepatoprotection. This study highlights the possible mechanism by which quercetin plays significant role in hepatoprotection. HPLC analysis revealed the abundance of quercetin in the fruit extracts of Gynocardia odorata and Diospyros malabarica, were isolated, purified and subjected to liver function analysis on Wistar rats. Post quercetin treatment improved liver function parameters in the hepatotoxic Wistar rats by augmenting bilirubin content, SGOT and SGPT activity. Gene expression profile of quercetin treated rats revealed down regulation of HGF, TIMP1 and MMP2 expressed during CCl4 induction. In silico molecular mechanism prediction suggested that quercetin has a high affinity for cell signaling pathway proteins BCL-2, JAK2 and Cytochrome P450 Cyp2E1, which all play a significant role in CCl4 induced hepatotoxicity. In silico molecular docking and molecular dynamics simulation have shown that quercetin has a plausible affinity for major signaling proteins in liver. MMGBSA studies have revealed high binding of quercetin (ΔG) -41.48±11.02, -43.53±6.55 and -39.89±5.78 kcal/mol, with BCL-2, JAK2 and Cyp2E1, respectively which led to better stability of the quercetin bound protein complexes. Therefore, quercetin can act as potent inhibitor against CCl4 induced hepatic injury by regulating BCL-2, JAK2 and Cyp2E1.
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Affiliation(s)
- Arabinda Ghosh
- Microbiology Division, Department of Botany, Gauhati University, Guwahati, Assam, India
| | - Pranjal Sarmah
- Department of Bioengineering and Technology, GUIST, Gauhati University, Guwahati, Assam, India
| | - Harun Patel
- R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharastra, India
| | - Nobendu Mukerjee
- Department of Microbiology; Ramakrishna Mission Vivekananda Centenary College, Khardaha, West Bengal, Kolkata, India
| | - Rajbardhan Mishra
- Laboratory of Immunotherapy, Institute of Microbiology v.v.i., Czech Academy of Sciences, Videnska, Prague, Czech Republic
| | - Saad Alkahtani
- Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
| | - Rajender S. Varma
- Regional Center of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute, Palacky University, Olomouc, Czech Republic
| | - Debabrat Baishya
- Department of Bioengineering and Technology, GUIST, Gauhati University, Guwahati, Assam, India
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Development of a Hepatoprotective Herbal Drug from Turnera diffusa. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2022; 2022:5114948. [PMID: 35047045 PMCID: PMC8763504 DOI: 10.1155/2022/5114948] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/18/2021] [Revised: 12/14/2021] [Accepted: 12/17/2021] [Indexed: 12/17/2022]
Abstract
The incidence of liver diseases, such as nonalcoholic fatty liver disease and drug-induced liver injury, continues to rise and is one of the leading causes of acute hepatitis. Current trends suggest that these types of conditions will increase in the coming years. There are few drugs available for the prevention or treatment of hepatic diseases, and there is a growing need for the development of safe hepatoprotective agents. The medicinal plant, Turnera diffusa, has many ethnopharmacological uses, one of which is the production of a flavonoid named hepatodamianol, which is the principal component responsible for this plant's hepatoprotective properties. In the present study, we describe the development and standardization of an active extract obtained from T. diffusa. We conducted nuclear magnetic resonance spectroscopy to identify hepatodamianol unambiguously in each sample. Using this extract, hepatoprotection could be demonstrated in vivo for the first time. The hepatoprotective effect did not display a significant difference in vivo when compared with silymarin used as a positive control at the same doses. Implementation of quality criteria used for standardization, such as flavonoid and hepatodamianol content, hepatoprotective activity, and absence of residual solvents, will allow future preclinical trials with this herbal drug.
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Ezhilarasan D, Raghunandhakumar S. Boldine treatment protects acetaminophen-induced liver inflammation and acute hepatic necrosis in mice. J Biochem Mol Toxicol 2021; 35:e22697. [PMID: 33393705 DOI: 10.1002/jbt.22697] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/10/2020] [Revised: 10/21/2020] [Accepted: 12/12/2020] [Indexed: 12/14/2022]
Abstract
Drug-induced liver injury (DILI) is a frequent cause responsible for acute liver failure (ALF). Acetaminophen (APAP) is a known hepatotoxin predictably causing intrinsic DILI. At high doses, APAP causes acute liver necrosis and responsible for ALF and liver transplant cases in 50% and 20% of patients, respectively, in the United States alone. Oxidative stress and glutathione depletion are implicated in APAP-induced liver necrosis. Boldine, a plant-derived compound is shown to have promising antioxidant potential. Therefore, this study investigates the protective effect of boldine against APAP-induced acute hepatic necrosis in mice. A single toxic dose of APAP (300 mg/kg b.w. p.o.) was administered in overnight-fasted mice to induce acute liver necrosis. Separately, APAP + boldine and APAP + N-acetylcysteine (NAC) simultaneous treatments were also given. Serum transaminases and reduced glutathione, enzymic antioxidants, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and, IL-6 were evaluated in liver tissue. Acute APAP intoxication significantly elevated serum marker enzymes of hepatotoxicity. APAP administration increased lipid peroxidation, TNF-α, IL-1β, and IL-6 protein expressions. The enzymic antioxidants and reduced glutathione levels were decreased in liver tissue of APAP intoxicated mice. Boldine and NAC simultaneous treatments prevented APAP-induced oxidative stress, inflammation, and necrosis. The results of this study suggest the crucial role of boldine to protect against APAP induced hepatotoxicity by virtue of its antioxidant and anti-inflammatory properties.
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Affiliation(s)
- Devaraj Ezhilarasan
- Department of Pharmacology, The Blue Lab (Molecular Pharmacology and Toxicology Division), Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.,Department of Pharmacology, Biomedical Research Unit and Laboratory Animal Centre, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India
| | - Subramanian Raghunandhakumar
- Department of Pharmacology, The Blue Lab (Molecular Pharmacology and Toxicology Division), Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India
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Cassels BK, Fuentes-Barros G, Castro-Saavedra S. Boldo, Its Secondary Metabolites and their Derivatives. CURRENT TRADITIONAL MEDICINE 2019. [DOI: 10.2174/2215083804666181113112928] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022]
Abstract
Boldo leaves (Boldo folium, from Peumus boldus Mol.) are very frequently used as a medicinal herb in Chile and are exported to many countries to be used in teas or as extracts included in herbal remedies, primarily as an aid to digestion and as a mild sedative. Scientific support for these uses is scanty, and boldine, an alkaloid viewed as characteristic of the tree and present in high concentration in the bark, is extracted by specialized companies and sold as the supposed main active constituent. Consequently, boldine has been the subject of a considerable number of research papers, while some of the other alkaloids present to a greater extent in the leaves have been relatively neglected except when found in large amounts in other species. These studies range from assays of antioxidant activity to anti-inflammatory, antineoplastic and other medical applications. The essential oil, usually containing a large percentage of the toxic ascaridole, was once used as a vermifuge and is now regarded with caution, but is still of interest as a possible natural insecticide, fungicide, antiparasitic and herbicide. The last decade has seen an explosive increase in papers pointing to possible uses of boldo and its constituents. This review attempts to bring these publications together in a comprehensive way with the purpose of stimulating and orienting further research into the useful properties of this Chilean endemic tree.
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Affiliation(s)
- Bruce K. Cassels
- Department of Chemistry, Faculty of Sciences, University of Chile, Santiago, Chile
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Lee Y, Cho IJ, Kim JW, Lee M, Ku SK, Choi J, Lee H. Hepatoprotective effects of blue honeysuckle on CCl 4-induced acute liver damaged mice. Food Sci Nutr 2019; 7:322-338. [PMID: 30680187 PMCID: PMC6341158 DOI: 10.1002/fsn3.893] [Citation(s) in RCA: 29] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/27/2018] [Revised: 10/23/2018] [Accepted: 11/03/2018] [Indexed: 12/20/2022] Open
Abstract
The objective of this study was to evaluate the hepatoprotective effects of blue honeysuckle (BH) on carbon tetrachloride (CCl4)-induced acute hepatic damage in mice. The experiment used a total of 60 ICR mice, which were divided into six groups. Except for the intact control groups, all groups received a single intraperitoneal injection of CCl4 after a 7 day pre-treatment period with distilled water, BH extracts, or silymarin. Twenty-four hours after the CCl4 injection, the following observations, representative of classical oxidative stress-mediated centrolobular necrotic acute liver injuries, were observed: decreased body weight; small nodule formation and enlargement on the gross inspections with related liver weight increase; elevation of serum AST and ALT, increases in hepatic lipid peroxidation and related depletion of endogenous antioxidants and antioxidative enzymes; centrolobular necrosis; increases in apoptotic markers, lipid peroxidation markers, and oxidative stress markers. However, liver damage was significantly inhibited by the pre-treatment with BH extracts. The present study demonstrated that oral administration of BH extracts prior to exposure to CCl4 conferred favorable hepatoprotective effects. These results demonstrated that BHe possessed suitable properties for use as a potent hepatoprotective medicinal food.
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Affiliation(s)
- You‐Suk Lee
- Department of Food and NutritionCollege of BioNano TechnologyGachon UniversitySeongnam‐siGyeonggi‐doKorea
| | - Il Je Cho
- The Medical Research Center for Globalization of Herbal FormulationDepartment of Herbal FormulationCollege of Oriental MedicineDaegu Haany UniversityGyeongsan‐siGyeongdanuk‐doKorea
| | | | - Min‐Ki Lee
- Department of Physical EducationKongju National UniversityKongju‐siChngcheongnam‐doKorea
| | - Sae Kwang Ku
- Department of Anatomy and HistologyCollege of Korean MedicineDaegu Haany UniversityGyeongsan‐siGyeongdanuk‐doKorea
| | - Jae‐Suk Choi
- Division of BioindustryCollege of Medical and Life SciencesSilla UniversityBusanKorea
| | - Hae‐Jeung Lee
- Department of Food and NutritionCollege of BioNano TechnologyGachon UniversitySeongnam‐siGyeonggi‐doKorea
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Yin L, Lu Q, Tan S, Ding L, Guo Y, Chen F, Tang L. Bioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata. Chem Cent J 2016; 10:27. [PMID: 27148400 PMCID: PMC4855496 DOI: 10.1186/s13065-016-0172-1] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/26/2015] [Accepted: 04/19/2016] [Indexed: 11/20/2022] Open
Abstract
Background Veronica ciliata Fisch., widely distributed in western China, has been traditionally used in Tibetan Medicine as a treatment for hepatitis, cholecystitis, rheumatism, and urticaria. However, V. ciliata Fisch. has not been subjected to detailed chemical constitution analysis and the bioactive studies were restricted to its crude extracts. It is necessary to investigate the active chemical components of these extracts and identify their biological effects. Results Four iridoid glycosides, (veronicoside, cataposide, amphicoside, and verminoside) were isolated from the ethyl acetate fraction. Among these compounds, veronicoside and verminoside were isolated for the first time from this plant. These compounds exhibited strong antioxidant activity and inhibitory activity on HepG2 cell proliferation. The antioxidant activity of verminoside was equal to Vc. Cataposide, amphicoside and verminoside had stronger anti-hepatocarcinoma activity than 5-fluorouracil. Conclusions Four iridoid glycosides,(veronicoside, cataposide, amphicoside and verminoside) were isolated from the extract of V. ciliata Fisch. using bioassay-guided screening.Among these compounds, veronicoside and verminoside were isolated for the first time from this plant. The above results indicated that these compounds were the active chemical components responsible for the antioxidant and anti-hepatocarcinoma properties of V. ciliata Fisch. The underlying mechanism of their bioactivity is worthy of further investigation.
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Affiliation(s)
- Li Yin
- College of Life Sciences, Sichuan University, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, No.24 South Sect. 1, Yihuan Road, Chengdu, People's Republic of China ; National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, Chengdu, Sichuan China
| | - Qiuxia Lu
- College of Life Sciences, Sichuan University, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, No.24 South Sect. 1, Yihuan Road, Chengdu, People's Republic of China ; National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, Chengdu, Sichuan China
| | - Shancai Tan
- College of Life Sciences, Sichuan University, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, No.24 South Sect. 1, Yihuan Road, Chengdu, People's Republic of China ; National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, Chengdu, Sichuan China
| | - Lisheng Ding
- Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, People's Republic of China
| | - Yiran Guo
- College of Life Sciences, Sichuan University, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, No.24 South Sect. 1, Yihuan Road, Chengdu, People's Republic of China ; National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, Chengdu, Sichuan China
| | - Fang Chen
- College of Life Sciences, Sichuan University, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, No.24 South Sect. 1, Yihuan Road, Chengdu, People's Republic of China ; National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, Chengdu, Sichuan China
| | - Lin Tang
- College of Life Sciences, Sichuan University, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, No.24 South Sect. 1, Yihuan Road, Chengdu, People's Republic of China ; National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, Chengdu, Sichuan China
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Zeng QP, Liu ZH, Huang AW, Zhang J, Song HT. Preparation and characterization of silymarin synchronized-release microporous osmotic pump tablets. DRUG DESIGN DEVELOPMENT AND THERAPY 2016; 10:519-31. [PMID: 26889080 PMCID: PMC4743634 DOI: 10.2147/dddt.s91571] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 01/28/2023]
Abstract
The pharmacological activity of herbal medicine is an overall action of each component in accordance with their original proportion. An efficient, sustained, and controlled-release drug delivery system of herbal medicine should ensure the synchronized drug release of each active component during the entire release procedure. In this study, silymarin (SM), a poorly soluble herbal medicine, was selected as a model drug to develop a synchronized-release drug delivery system: an SM microporous osmotic pump (MPOP) tablet. The SM was conjugated with phospholipid (SM phytosome complex, SM-PC) to improve the solubility, and the difference in the apparent octanol-water partition coefficient between the two components was significantly reduced. The dissolution rate of SM-PC was significantly higher than SM active pharmaceutical ingredients and was the same as that of the commercial SM capsule. The SM-PC was used to generate the MPOP tablet. SM was mixed with poly(ethylene) oxide and sodium chloride (an osmotic agent) to form the MPOP core, followed by coating with cellulose acetate and poly(ethylene) oxide to generate the SM MPOP. The results demonstrated that SM MPOP could synchronically and sustainably release the five active components within 12 hours (the similar coefficient f 2 between two components was >65), and the average cumulative release rate was 85%. Fitting of the drug-release curve showed a zero-order release profile for SM MPOP. Our study showed that the phytosome complex technique combined with the MPOP system will achieve synchronized release of the various active components of herbal medicine and have potential applications in developing sustained release preparations in herbal medicine.
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Affiliation(s)
- Qi-ping Zeng
- Department of Pharmacy, Fuzhou General Hospital of Nanjing Command PLA, Fuzhou, People's Republic of China
| | - Zhi-hong Liu
- Department of Pharmacy, Fuzhou General Hospital of Nanjing Command PLA, Fuzhou, People's Republic of China
| | - Ai-wen Huang
- Department of Pharmacy, Fuzhou General Hospital of Nanjing Command PLA, Fuzhou, People's Republic of China
| | - Jing Zhang
- Department of Pharmacy, Fuzhou General Hospital of Nanjing Command PLA, Fuzhou, People's Republic of China
| | - Hong-tao Song
- Department of Pharmacy, Fuzhou General Hospital of Nanjing Command PLA, Fuzhou, People's Republic of China
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Chatterjee A, Acharya K. Include mushroom in daily diet—A strategy for better hepatic health. FOOD REVIEWS INTERNATIONAL 2015. [DOI: 10.1080/87559129.2015.1057839] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
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Hepatoprotective Effect of Silymarin (Silybum marianum) on Hepatotoxicity Induced by Acetaminophen in Spontaneously Hypertensive Rats. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2015; 2015:538317. [PMID: 25821491 PMCID: PMC4363982 DOI: 10.1155/2015/538317] [Citation(s) in RCA: 49] [Impact Index Per Article: 4.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/17/2014] [Revised: 01/20/2015] [Accepted: 01/21/2015] [Indexed: 01/19/2023]
Abstract
This study was aimed to investigate the effect of Silymarin (SLM) on the hypertension state and the liver function changes induced by acetaminophen (APAP) in spontaneously hypertensive rat (SHR). Animals normotensive (N) or hypertensive (SHR) were treated or not with APAP (3 g/kg, oral) or previously treated with SLM. Twelve hours after APAP administration, plasmatic levels of liver function markers: alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose (GLU), gamma glutamyl transferase (γ-GT), and alkaline phosphatase (ALP) of all groups, were determined. Liver injury was assessed using histological studies. Samples of their livers were then used to determine the myeloperoxidase (MPO) activity and nitric oxide (NO) production and were also sectioned for histological analysis. No differences were observed for ALT, γ-GT, and GLU levels between SHR and normotensive rats groups. However, AST and ALP levels were increased in hypertensive animals. APAP treatment promoted an increase in ALT and AST in both SHR and N. However, only for SHR, γ-GT levels were increased. The inflammatory response evaluated by MPO activity and NO production showed that SHR was more susceptible to APAP effect, by increasing leucocyte infiltration. Silymarin treatment (Legalon) restored the hepatocyte functional and histopathological alterations induced by APAP in normotensive and hypertensive animals.
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Anuf AR, Ramachandran R, Krishnasamy R, Gandhi PSS, Periyasamy S. Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines. Pharmacognosy Res 2014; 6:312-9. [PMID: 25276069 PMCID: PMC4166820 DOI: 10.4103/0974-8490.138280] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2014] [Revised: 05/20/2014] [Accepted: 08/06/2014] [Indexed: 11/04/2022] Open
Abstract
BACKGROUND Plumbago rosea is used in traditional systems of medicine for the preparation of formulations used for treating inflammations, cough, bronchitis, and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. In the present study, the cytotoxic and anti-proliferative effects of plumbagin, and the ethanolic root extract of P. rosea (ETPR) was evaluated on SK-MEL 28 melanoma cell lines and human lymphocytes. MATERIALS AND METHODS MTT and apoptotic assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. In addition, the effect of Plumbagin and ETPR in down regulation of BCL-2 expression is investigated using RT-PCR analysis. RESULTS Both plumbagin and ETPR dose-dependently decreased the cell viability more potently in melanoma cell lines. P. rosea extract demonstrated significant synergy in inhibiting BCL-2 expression than plumbagin. Moreover plumbagin showed more toxicity in human lymphocytes. CONCLUSION Plumbagin has anti-cancer potential, but the side effects limits its use; yet plumbagin, in combination with other ingredients in Plumbago rosea extract, displays significant synergy leading to a stronger anticancer effect with significantly less toxicity.
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Affiliation(s)
- Alexander Ronaldo Anuf
- Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar,Tamil Nadu, India
| | | | - Rajaram Krishnasamy
- Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India
| | - P S Sudhakar Gandhi
- Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India
| | - Sureshkumar Periyasamy
- Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India
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Joseph JA, Ayyappan UPT, Sasidharan SR, Mutyala S, Goudar KS, Agarwal A. Ameliorative effect of Phytocee™ Cool against carbon tetrachloride-induced oxidative stress. Pharmacognosy Res 2014; 6:320-5. [PMID: 25276070 PMCID: PMC4166821 DOI: 10.4103/0974-8490.138284] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/19/2014] [Revised: 04/16/2014] [Accepted: 08/06/2014] [Indexed: 01/28/2023] Open
Abstract
BACKGROUND Antioxidants from natural sources have a major role in reversing the effects of oxidative stress and promoting health, growth and productivity in animals. OBJECTIVE This study was undertaken to investigate the possible antioxidant activity and hepatoprotective effects of Phytocee™ Cool on carbon tetrachloride (CCl4) induced oxidative stress and liver damage in rats. MATERIALS AND METHODS Animals were pretreated with Phytocee™ Cool for 10 days and were challenged with CCl4 (1:1 v/v) in olive oil on the 10(th) day. After 24 h of CCl4 administration blood was collected and markers of hepatocellular damage aspartate aminotransferase (AST), alanine aminotransferase (ALT) were evaluated. Rats were sacrificed and oxidative stress in liver was estimated using malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase. RESULTS CCl4 caused a significant increase in serum AST, ALT, hepatic MDA and GSH levels, whereas the SOD and catalase activities were decreased. Phytocee™ Cool pretreatment attenuated the MDA, AST ALT levels and increased the activities of SOD and catalase. CONCLUSION Phytocee™ Cool demonstrated antioxidant potential and hepatoprotective effects and plausibly be used in the amelioration of oxidative stress.
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Affiliation(s)
- Joshua Allan Joseph
- Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India
| | | | | | - Sridhar Mutyala
- Department of Pharmacology and Toxicology, R&D Centre, Natural Remedies, Bangalore, Karnataka, India
| | | | - Amit Agarwal
- Department of Pharmacology and Toxicology, R&D Centre, Natural Remedies, Bangalore, Karnataka, India
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Lied GA, Gilja OH, Hausken T. Har silibinin en plass i behandlingen av leversykdommer? TIDSSKRIFT FOR DEN NORSKE LEGEFORENING 2014; 134:392. [DOI: 10.4045/tidsskr.13.1531] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/02/2022] Open
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