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For: Awodele O, Oreagba IA, Odoma S, da Silva JAT, Osunkalu VO. Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). J Ethnopharmacol 2012;139:330-336. [PMID: 22138517 DOI: 10.1016/j.jep.2011.10.008] [Citation(s) in RCA: 92] [Impact Index Per Article: 7.1] [Reference Citation Analysis] [What about the content of this article? (0)] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/22/2011] [Revised: 08/03/2011] [Accepted: 10/03/2011] [Indexed: 05/31/2023]

For:	Awodele O, Oreagba IA, Odoma S, da Silva JAT, Osunkalu VO. Toxicological evaluation of the aqueous leaf extract of Moringa oleifera Lam. (Moringaceae). J Ethnopharmacol 2012;139:330-336. [PMID: 22138517 DOI: 10.1016/j.jep.2011.10.008] [Citation(s) in RCA: 92] [Impact Index Per Article: 7.1] [Reference Citation Analysis] [What about the content of this article? (0)] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/22/2011] [Revised: 08/03/2011] [Accepted: 10/03/2011] [Indexed: 05/31/2023]

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Cited by Other Article(s)

Anyanwu GO, Anzaku D, Bulus YJ, Girgi JN, Donwell CC, Ihuma JO, Onyeneke EC, Bermano G, Steenkamp V. An Ethnobotanical Survey and Pharmacological and Toxicity Review of Medicinal Plants Used in the Management of Obesity in the North Central Zone of Nigeria. J Obes 2025;2025:5568216. [PMID: 40026359 PMCID: PMC11870763 DOI: 10.1155/jobe/5568216] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/01/2023] [Revised: 12/17/2024] [Accepted: 12/31/2024] [Indexed: 03/05/2025] Open

Abstract

Introduction: Obesity is increasing worldwide. Due to the unavailability of affordable obesity drugs in most parts of Nigeria, many overweight and obese people rely on medicinal plants to manage obesity. Thus, the aim of this study is to document medicinal plants traditionally used in the treatment and management of obesity in the North Central Zone of Nigeria, determine the plants to which pharmacological assessment of their use in obesity management has not been reported, and assess their toxicity based on the literature. Methods: Semistructured questionnaires and interviews were used to assess sociodemographic information of the 700 herb sellers/practitioners (100 for each state) who consented to participate in the study. Information gathered on plants that are traditionally used in the management of obesity included administration/dosage, method of preparation, plant part used, method of growth, and plant type. The field study was conducted over a one-year period, from March 2018 to March 2019. Reports of pharmacological activity pertaining to obesity as well as toxicity of the plants were obtained from the literature via scientific databases (Scopus, Web of Science, PubMed, Google Scholar, SciFinder, AJOL, PubChem, and other web sources) after the field survey. Results: A total of 39 families and 70 plant species were used to treat or manage obesity. The majority of plant species used resulted in the family Leguminosae. The relative frequency of citation (RFC) and percentage values for the five most frequently used plants were as follows: Citrus aurantifolia (0.0500; 3.56%), Citrus limon (0.0457; 3.26%), Garcinia kola (0.0429; 3.05%), Zingiber officinale (0.0429; 3.05%), and Allium sativum (0.0414; 2.95%). The majority of the medications were prepared as decoctions (50.5%), and cultivated plants (62.86%) were in the majority of plants used. Results showed that 23 plants have no pharmacological report for antiobesity activities while among the five frequently used plants, only Garcinia kola was reported toxic in preclinical models. Conclusions: This paper provides a valuable compilation of the plants used in obesity treatment in the study area by indigenous healers, highlights plants with no reported pharmacological activity pertaining to obesity, and indicates the toxicity profile of used plants. However, further studies on the mechanism of action are warranted, especially where no reports were obtained.

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He M, Zhang Y, Zhai Y, Li Y, Yang G, Yu S, Xiao H, Song Y. Trilobatin regulates glucose metabolism by ameliorating oxidative stress and insulin resistance in vivo and in vitro. J Pharm Pharmacol 2025;77:236-248. [PMID: 38642915 DOI: 10.1093/jpp/rgae035] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/22/2023] [Accepted: 03/15/2024] [Indexed: 04/22/2024]

Abstract

OBJECTIVES

Trilobatin, a glycosylated dihydrochalcone, has been reported to have anti-diabetic properties. However, the underlying mechanism remains unexplained.

METHODS

In this investigation, the regulation of trilobatin on glucose metabolism of insulin resistance (IR)-HepG2 cells and streptozocin (STZ)-induced mice and its mechanism were evaluated.

KEY FINDINGS

Different doses of trilobatin (5, 10 and 20 μM) increased glucose consumption, glycogen content, hexokinase (HK), and pyruvate kinase (PK) activity in IR-HepG2 cells. Among them, the HK and PK activity in IR-HepG2 cells treated with 20 μM trilobatin were 1.84 and 2.05 times than those of the IR-group. The overeating, body and tissue weight, insulin levels, liver damage, and lipid accumulation of STZ-induced mice were improved after feeding with different doses of trilobatin (10, 50, and 100 mg/kg/d) for 4 weeks. Compared with STZ-induced mice, fasting blood glucose decreased by 61.11% and fasting insulin (FINS) increased by 48.6% after feeding trilobatin (100 mg/kg/d). Meanwhile, data from quantitative real-time polymerase chain reaction (qRT-PCR) revealed trilobatin ameliorated glycogen synthesis via the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) signaling pathway in IR-HepG2 cells and in STZ-induced mice. Furthermore, in vitro and in vivo experiments showed that trilobatin ameliorated oxidative stress by regulating the mRNA expression of nuclear erythroid-2 related factor 2 (Nrf2)/kelch-like ECH associated protein-1 (Keap-1) pathway as well as heme oxygenase-1 (HO-1) and NAD(P)H: quinone oxidoreductase-1 (NQO-1).

CONCLUSIONS

Our research reveals a novel pharmacological activity of trilobatin: regulating glucose metabolism through PI3K/Akt/GSK-3β and Nrf2/Keap-1 signaling pathways, improving insulin resistance and reducing oxidative stress. Trilobatin can be used as a reliable drug resource for the treatment of glucose metabolism disorders.

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Kumar H, Guleria S, Dhalaria R, Nepovimova E, Bhardwaj N, Jha P, Dhanjal DS, Verma N, Malik T. Valorization of Moringa oleifera Lam.: Healthy green biomass for circular bioeconomy. Food Chem X 2025;26:102358. [PMID: 40129732 PMCID: PMC11931315 DOI: 10.1016/j.fochx.2025.102358] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/14/2024] [Revised: 03/06/2025] [Accepted: 03/07/2025] [Indexed: 03/26/2025] Open

Mohammed DM, Yang X, El-Messery TM, Jiang X, Zahran HA, Gebremeskal YH, Farouk A. Bioactive Moringa oleifera and Nigella sativa oils microcapsules alleviate high-fat-diet induced hepatic oxidative damage and inflammation in rats. FOOD BIOSCI 2025;64:105873. [DOI: 10.1016/j.fbio.2025.105873] [Citation(s) in RCA: 3] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/03/2025]

Shah K, Jalgaonkar M, Vyas A, Doshi G, Kulkarni YA, Singh AD, Oza MJ. A herbal approach to diabetic cardiomyopathy: moringa, ginger, and garlic unveiled. Arch Physiol Biochem 2025:1-10. [PMID: 39888842 DOI: 10.1080/13813455.2025.2459871] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/16/2024] [Revised: 12/18/2024] [Accepted: 01/23/2025] [Indexed: 02/02/2025]

Thibault TH, Yannick E, Abassi D, Cédric B, Mohamed H, François C. Unveiling the potential and specificity of the Mahoran ethnopharmacopoeia: A field survey. JOURNAL OF ETHNOPHARMACOLOGY 2025;340:119255. [PMID: 39694429 DOI: 10.1016/j.jep.2024.119255] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/29/2024] [Revised: 11/14/2024] [Accepted: 12/15/2024] [Indexed: 12/20/2024]

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

A significant portion of Mahoran people relies on traditional medicine to address their healthcare needs. However, very few studies have been carried out on this subject, and few data are available on the practices, plants used, and ailments most commonly treated by their traditional medicine.

AIM OF THE STUDY

Within this context, the aim of this study was to identify the diseases most commonly treated by traditional Mahoran medicine, as well as the plants most commonly used against these various ailments.

MATERIALS AND METHODS

From January to April 2023, a semi-structured survey was carried out in Mayotte island. A total of 103 participants were interviewed including 65 non specialists, 21 knowledgeable, and 17 specialists. A thorough literature review was performed on the most cited plant species to evaluate the benefit-risk of each remedy.

RESULTS

Participants mentioned using 474 remedies (prepared mostly with herbal ingredients) to treat 65 diseases. These diseases belong to various health categories of which the most represented ones were digestive system, respiratory system, genital system, general, and muscular system. The two most common ailments cited by participants were stomachache (41/103) and cough (36/103). A total of 154 plant species were identified, with Coleus amboinicus, Citrus aurantiifolia, Moringa oleifera, and Ocimum gratissimum being the main plants reported. Massage therapy was the second most important traditional practices reported after the use of herbal remedies.

CONCLUSION

Our survey confirms the importance of traditional medical pratices in Mayotte island. The following plants: Aerva lanata, Cardiospermum halicacabum, Coleus madagascariensis Paullinia pinnata, and Woodfordia fruticosa stand out from the others in terms of their use and number of citations, and it would be interesting to study their pharmacological and toxicological properties. Traditional medicine in Mayotte also possesses specificities, notably with the use of particular ingredients such as salt, coral stone, or even white clay. Furthermore, throughout the study, we noticed that chronic diseases such as diabetes or hypertension were extensively treated. This could be linked to the fact that the prevalence of these diseases is quite high on the island.

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Ali L, Ahmad N, Uddin MN, Abdel-Maksoud MA, Fazal H, Fatima S, El-Tayeb MA, Kiani BH, Khan W, Rahat MA, Ali M, Khan Y, Rauf K, Khan S, Ullah S, Ahmad T, Salam A, Ahmad S. Immobilization of Silver Nanoparticles with Defensive Gum of Moringa oleifera for Antibacterial Efficacy Against Resistant Bacterial Species from Human Infections. Pharmaceuticals (Basel) 2024;17:1546. [PMID: 39598455 PMCID: PMC11597128 DOI: 10.3390/ph17111546] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/25/2024] [Revised: 10/29/2024] [Accepted: 11/11/2024] [Indexed: 11/29/2024] Open

Affiliation(s)

Liaqat Ali Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Nisar Ahmad Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Muhammad Nazir Uddin Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Mostafa A. Abdel-Maksoud Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;
Hina Fazal Pakistan Council for Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar 25120, Pakistan
Sabiha Fatima Department of Clinical Laboratory Science, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 12371, Saudi Arabia; (S.F.); (M.A.E.-T.)
Mohamed A. El-Tayeb Department of Clinical Laboratory Science, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 12371, Saudi Arabia; (S.F.); (M.A.E.-T.)
Bushra Hafeez Kiani Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609, USA;
Wajid Khan Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Murad Ali Rahat Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Mohammad Ali Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Yaqub Khan Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Kamran Rauf Department of Horticulture, The University of Agriculture Peshawar, Peshawar 25120, Pakistan;
Salman Khan Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Sami Ullah Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Tanveer Ahmad Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Afshan Salam Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)
Sajjad Ahmad Centre for Biotechnology and Microbiology, University of Swat, Swat 19120, Pakistan; (L.A.); (M.N.U.); (W.K.); (M.A.R.); (M.A.); (Y.K.); (S.K.); (S.U.); (T.A.); (A.S.); (S.A.)

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Alves RRDV, de Oliveira AM, dos Prazeres GB, da Silva AR, Costa FF, Barros BRDS, Souza TGDS, Coelho LCBB, de Melo CML, Ferreira MRA, Soares LAL, Chagas CA, Macedo MLR, Napoleão TH, Fernandes MP, Paiva PMG. Evaluation of Cytotoxicity and Acute Oral Toxicity of Saline Extract and Protein-Rich Fraction from Moringa oleifera Lam. Leaves. Pharmaceuticals (Basel) 2024;17:1045. [PMID: 39204150 PMCID: PMC11357182 DOI: 10.3390/ph17081045] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/07/2024] [Revised: 06/25/2024] [Accepted: 07/03/2024] [Indexed: 09/03/2024] Open

Affiliation(s)

Robson Raion de Vasconcelos Alves Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (R.R.d.V.A.); (A.M.d.O.); (G.B.d.P.); (A.R.d.S.); (L.C.B.B.C.); (T.H.N.)
Alisson Macário de Oliveira Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (R.R.d.V.A.); (A.M.d.O.); (G.B.d.P.); (A.R.d.S.); (L.C.B.B.C.); (T.H.N.)
Gabryella Borges dos Prazeres Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (R.R.d.V.A.); (A.M.d.O.); (G.B.d.P.); (A.R.d.S.); (L.C.B.B.C.); (T.H.N.)
Abdênego Rodrigues da Silva Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (R.R.d.V.A.); (A.M.d.O.); (G.B.d.P.); (A.R.d.S.); (L.C.B.B.C.); (T.H.N.)
Franciele Florencio Costa Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (F.F.C.); (M.R.A.F.); (L.A.L.S.)
Bárbara Rafaela da Silva Barros Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (B.R.d.S.B.); (C.M.L.d.M.)
Talita Giselly dos Santos Souza Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, PE, Brazil; (T.G.d.S.S.); (C.A.C.); (M.P.F.)
Luana Cassandra Breintenbach Barroso Coelho Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (R.R.d.V.A.); (A.M.d.O.); (G.B.d.P.); (A.R.d.S.); (L.C.B.B.C.); (T.H.N.)
Cristiane Moutinho Lagos de Melo Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (B.R.d.S.B.); (C.M.L.d.M.)
Magda Rhayanny Assunção Ferreira Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (F.F.C.); (M.R.A.F.); (L.A.L.S.)
Luiz Alberto Lira Soares Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (F.F.C.); (M.R.A.F.); (L.A.L.S.)
Cristiano Aparecido Chagas Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, PE, Brazil; (T.G.d.S.S.); (C.A.C.); (M.P.F.)
Maria Lígia Rodrigues Macedo Departamento de Tecnologia de Alimentos e da Saúde, Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal do Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil;
Thiago Henrique Napoleão Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (R.R.d.V.A.); (A.M.d.O.); (G.B.d.P.); (A.R.d.S.); (L.C.B.B.C.); (T.H.N.)
Mariana Pinheiro Fernandes Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, PE, Brazil; (T.G.d.S.S.); (C.A.C.); (M.P.F.)
Patrícia Maria Guedes Paiva Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil; (R.R.d.V.A.); (A.M.d.O.); (G.B.d.P.); (A.R.d.S.); (L.C.B.B.C.); (T.H.N.)

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Bagri P, Kumar V, Batra K. Assessment of cytoprotective and genoprotective effects of Moringa oleifera and Tinospora cordifolia extracts in vitro. Toxicol Res (Camb) 2024;13:tfae133. [PMID: 39184218 PMCID: PMC11339162 DOI: 10.1093/toxres/tfae133] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/16/2023] [Revised: 08/01/2024] [Accepted: 08/12/2024] [Indexed: 08/27/2024] Open

Palomino-Pacheco M, Rojas-Armas JP, Ortiz-Sánchez JM, Arroyo-Acevedo JL, Justil-Guerrero HJ, Martínez-Heredia JT. Assessment of oral toxicity of Moringa oleifera Lam aqueous extract and its effect on gout induced in a murine model. Vet World 2024;17:1449-1458. [PMID: 39185060 PMCID: PMC11344109 DOI: 10.14202/vetworld.2024.1449-1458] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/05/2024] [Accepted: 06/07/2024] [Indexed: 08/27/2024] Open

Camilleri E, Blundell R. A comprehensive review of the phytochemicals, health benefits, pharmacological safety and medicinal prospects of Moringaoleifera. Heliyon 2024;10:e27807. [PMID: 38496871 PMCID: PMC10944276 DOI: 10.1016/j.heliyon.2024.e27807] [Citation(s) in RCA: 7] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/01/2023] [Revised: 02/23/2024] [Accepted: 03/06/2024] [Indexed: 03/19/2024] Open

Klimek-Szczykutowicz M, Gaweł-Bęben K, Rutka A, Blicharska E, Tatarczak-Michalewska M, Kulik-Siarek K, Kukula-Koch W, Malinowska MA, Szopa A. Moringa oleifera (drumstick tree)-nutraceutical, cosmetological and medicinal importance: a review. Front Pharmacol 2024;15:1288382. [PMID: 38370483 PMCID: PMC10869624 DOI: 10.3389/fphar.2024.1288382] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2023] [Accepted: 01/05/2024] [Indexed: 02/20/2024] Open

Adarthaiya S, Sehgal A. Moringa oleifera Lam. as a potential plant for alleviation of the metabolic syndrome-A narrative review based on in vivo and clinical studies. Phytother Res 2024;38:755-775. [PMID: 38015048 DOI: 10.1002/ptr.8079] [Citation(s) in RCA: 3] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/27/2023] [Revised: 11/06/2023] [Accepted: 11/10/2023] [Indexed: 11/29/2023]

Saleem A, Khalid H, Akhtar MF, Zeb A. Appraisal of anti-arthritic potential of Coronopus didymus (L.) Sm. aqueous extract and its safety study in Wistar rats. Inflammopharmacology 2023;31:3167-3182. [PMID: 37962695 DOI: 10.1007/s10787-023-01374-y] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/26/2023] [Accepted: 10/09/2023] [Indexed: 11/15/2023]

Alam MN, Singh L, Khan NA, Asiri YI, Hassan MZ, Afzal O, Altamimi ASA, Hussain MS. Ameliorative Effect of Ethanolic Extract of Moringa oleifera Leaves in Combination with Curcumin against PTZ-Induced Kindled Epilepsy in Rats: In Vivo and In Silico. Pharmaceuticals (Basel) 2023;16:1223. [PMID: 37765031 PMCID: PMC10534968 DOI: 10.3390/ph16091223] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/28/2023] [Revised: 07/30/2023] [Accepted: 08/09/2023] [Indexed: 09/29/2023] Open

Abstract

The ameliorative effect of ethanolic extract of M. oleifera (MOEE) leaves in combination with curcumin against seizures, cognitive impairment, and oxidative stress in the molecular docking of PTZ-induced kindled rats was performed to predict the potential phytochemical effects of MOEE and curcumin against epilepsy. The effect of pretreatment with leaves of M. oleifera ethanolic extracts (MOEE) (250 mg/kg and 500 mg/kg, orally), curcumin (200 mg/kg and 300 mg/kg, orally), valproic acid used as a standard (100 mg/kg), and the combined effect of MOEE (250 mg/kg) and curcumin (200 mg/kg) at a low dose on Pentylenetetrazole was used for (PTZ)-induced kindling For the development of kindling, individual Wistar rats (male) were injected with pentyletetrazole (40 mg/kg, i.p.) on every alternate day. Molecular docking was performed by the Auto Dock 4.2 tool to merge the ligand orientations in the binding cavity. From the RCSB website, the crystal structure of human glutathione reductase (PDB ID: 3DK9) was obtained. Curcumin and M. oleifera ethanolic extracts (MOEE) showed dose-dependent effects. The combined effects of MOEE and curcumin leaves significantly improved the seizure score and decreased the number of myoclonic jerks compared with a standard dose of valproic acid. PTZ kindling induced significant oxidative stress and cognitive impairment, which was reversed by pretreatment with MOEE and curcumin. Glutathione reductase (GR) is an enzyme that plays a key role in the cellular control of reactive oxygen species (ROS). Therefore, activating GR can uplift antioxidant properties, which leads to the inhibition of ROS-induced cell death in the brain. The combination of the ethanolic extract of M. oleifera (MOEE) leaves and curcumin has shown better results than any other combination for antiepileptic effects by virtue of antioxidant effects. As per the docking study, chlorogenic acid and quercetin treated with acombination of curcumin have much more potential.

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Falode JA, Ajayi OI, Isinkaye TV, Adeoye AO, Ajiboye BO, Brai BIC, ADEOYE, Basiru Olaitan, AJIBOYE, BRAI BIC. Justicia carnea extracts ameliorated hepatocellular damage in streptozotocin-induced type 1 diabetic male rats via decrease in oxidative stress, inflammation and increasing other risk markers. Biomarkers 2023;28:177-189. [PMID: 36511112 DOI: 10.1080/1354750x.2022.2157487] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]

Potential of moringa (Moringa oleifera) leaf powder for functional food ingredients: A review. CZECH JOURNAL OF FOOD SCIENCES 2023. [DOI: 10.17221/221/2022-cjfs] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/11/2023]

Moringa oleifera leaf protein: extraction, characteristics and applications. J Food Compost Anal 2023. [DOI: 10.1016/j.jfca.2023.105234] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/24/2023]

Pareek A, Pant M, Gupta MM, Kashania P, Ratan Y, Jain V, Pareek A, Chuturgoon AA. Moringa oleifera: An Updated Comprehensive Review of Its Pharmacological Activities, Ethnomedicinal, Phytopharmaceutical Formulation, Clinical, Phytochemical, and Toxicological Aspects. Int J Mol Sci 2023;24:ijms24032098. [PMID: 36768420 PMCID: PMC9916933 DOI: 10.3390/ijms24032098] [Citation(s) in RCA: 57] [Impact Index Per Article: 28.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/29/2022] [Revised: 01/09/2023] [Accepted: 01/13/2023] [Indexed: 01/21/2023] Open

Abstract

Moringa oleifera, also known as the "tree of life" or "miracle tree," is classified as an important herbal plant due to its immense medicinal and non-medicinal benefits. Traditionally, the plant is used to cure wounds, pain, ulcers, liver disease, heart disease, cancer, and inflammation. This review aims to compile an analysis of worldwide research, pharmacological activities, phytochemical, toxicological, and ethnomedicinal updates of Moringa oleifera and also provide insight into its commercial and phytopharmaceutical applications with a motive to help further research. The scientific information on this plant was obtained from various sites and search engines such as Scopus, Pub Med, Science Direct, BMC, Google Scholar, and other scientific databases. Articles available in the English language have only been referred for review. The pharmacological studies confirm the hepatoprotective, cardioprotective, and anti-inflammatory potential of the extracts from the various plant parts. It was found that bioactive constituents are present in every part of the plant. So far, more than one hundred compounds from different parts of Moringa oleifera have been characterized, including alkaloids, flavonoids, anthraquinones, vitamins, glycosides, and terpenes. In addition, novel isolates such as muramoside A&B and niazimin A&B have been identified in the plant and have potent antioxidant, anticancer, antihypertensive, hepatoprotective, and nutritional effects. The traditional and nontraditional use of Moringa, its pharmacological effects and their phytopharmaceutical formulations, clinical studies, toxicity profile, and various other uses are recognized in the present review. However, several traditional uses have yet to be scientifically explored. Therefore, further studies are proposed to explore the mechanistic approach of the plant to identify and isolate active or synergistic compounds behind its therapeutic potential.

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Azlan UK, Khairul Annuar NA, Mediani A, Aizat WM, Damanhuri HA, Tong X, Yanagisawa D, Tooyama I, Wan Ngah WZ, Jantan I, Hamezah HS. An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms of Moringa oleifera. Front Pharmacol 2023;13:1035220. [PMID: 36686668 PMCID: PMC9849397 DOI: 10.3389/fphar.2022.1035220] [Citation(s) in RCA: 12] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/02/2022] [Accepted: 12/20/2022] [Indexed: 01/06/2023] Open

Abstract

Neurodegenerative diseases (NDs) are sporadic maladies that affect patients' lives with progressive neurological disabilities and reduced quality of life. Neuroinflammation and oxidative reaction are among the pivotal factors for neurodegenerative conditions, contributing to the progression of NDs, such as Parkinson's disease (PD), Alzheimer's disease (AD), multiple sclerosis (MS) and Huntington's disease (HD). Management of NDs is still less than optimum due to its wide range of causative factors and influences, such as lifestyle, genetic variants, and environmental aspects. The neuroprotective and anti-neuroinflammatory activities of Moringa oleifera have been documented in numerous studies due to its richness of phytochemicals with antioxidant and anti-inflammatory properties. This review highlights up-to-date research findings on the anti-neuroinflammatory and neuroprotective effects of M. oleifera, including mechanisms against NDs. The information was gathered from databases, which include Scopus, Science Direct, Ovid-MEDLINE, Springer, and Elsevier. Neuroprotective effects of M. oleifera were mainly assessed by using the crude extracts in vitro and in vivo experiments. Isolated compounds from M. oleifera such as moringin, astragalin, and isoquercitrin, and identified compounds of M. oleifera such as phenolic acids and flavonoids (chlorogenic acid, gallic acid, ferulic acid, caffeic acid, kaempferol, quercetin, myricetin, (-)-epicatechin, and isoquercitrin) have been reported to have neuropharmacological activities. Therefore, these compounds may potentially contribute to the neuroprotective and anti-neuroinflammatory effects. More in-depth studies using in vivo animal models of neurological-related disorders and extensive preclinical investigations, such as pharmacokinetics, toxicity, and bioavailability studies are necessary before clinical trials can be carried out to develop M. oleifera constituents into neuroprotective agents.

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Malik A, Khan A, Mahmood Q, Nawaz Marth MM, Riaz M, Tabassum T, Rasool G, Rehman MFU, Batool AI, Kanwal F, Cai R. In Vivo and In Silico Assessment of the Cardioprotective Effect of Thymus linearis Extract against Ischemic Myocardial Injury. ACS OMEGA 2022;7:43635-43646. [PMID: 36506215 PMCID: PMC9730472 DOI: 10.1021/acsomega.2c04544] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 07/19/2022] [Accepted: 10/25/2022] [Indexed: 06/17/2023]

Singh J, Gautam DNS, Sourav S, Sharma R. Role of Moringa oleifera Lam. in cancer: Phytochemistry and pharmacological insights. FOOD FRONTIERS 2022. [DOI: 10.1002/fft2.181] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022] Open

Bancessi A, Teodósio R, Duarte E, Baldé A, Catarino L, Nazareth T. Moringa as a household water purification method - community perception and pilot study in Guinea-Bissau. BMC Public Health 2022;22:1953. [PMID: 36271392 PMCID: PMC9587536 DOI: 10.1186/s12889-022-14344-w] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/21/2022] [Accepted: 10/13/2022] [Indexed: 11/21/2022] Open

Abstract

Background

Public perceptions of water-related issues are still under-researched topics. The current paper intends to explore a local community’s perceptions regarding household water purification (HWP) strategies, namely before and after trying a new method: moringa seeds powder (moringa-teabag).

Methods

In September 2020, six focus group discussions (N = 65) assessing perceptions about the usefulness of Moringa oleifera Lam (Moringaceae) as a HWP method (before moringa-based HWP trials), and questionnaires (N = 104) evaluating successes and identifying difficulties (after one week of moringa-based HWP trials). Participants were all women aged over 18 years, living in Ondame, Biombo region, Guinea-Bissau. Data were analyzed using qualitative and quantitative approaches.

Results

The focus group discussions revealed that people are aware of the fact that water can transmit diseases. Although certain persons showed concern about shallow well water safety, people generally underestimate the risk, as they trust tubewell water. Not everyone had an understanding of what water contamination is, or the concept of medical importance. Some respondents declared they use traditional methods such as boiling and bleach to treat water before drinking. However, those who reported no kind of treatment indicated reasons such as lack of time, cost, and bleach’s taste and smell. In the questionnaire, more than half of the participants (68%) reported treating water before consumption. Nevertheless, these results are not consistent with our field notes. Participants demonstrated a strong belief in the capacity of moringa-teabags to purify water and even consider them better or much better (81%) than other methods. Participants asked for more information on moringa-teabag for household water purification.

Conclusion

More information on water treatment and water safety would help to raise public awareness about waterborne diseases. These findings could be used to promote greater adherence to moringa-based HWP as an alternative to household water treatment.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12889-022-14344-w.

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A Comprehensive Review with Updated Future Perspectives on the Ethnomedicinal and Pharmacological Aspects of Moringa oleifera. Molecules 2022;27:molecules27185765. [PMID: 36144493 PMCID: PMC9504211 DOI: 10.3390/molecules27185765] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/16/2022] [Revised: 08/30/2022] [Accepted: 09/02/2022] [Indexed: 11/17/2022] Open

Mthiyane FT, Dludla PV, Ziqubu K, Mthembu SXH, Muvhulawa N, Hlengwa N, Nkambule BB, Mazibuko-Mbeje SE. A Review on the Antidiabetic Properties of Moringa oleifera Extracts: Focusing on Oxidative Stress and Inflammation as Main Therapeutic Targets. Front Pharmacol 2022;13:940572. [PMID: 35899107 PMCID: PMC9310029 DOI: 10.3389/fphar.2022.940572] [Citation(s) in RCA: 18] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/10/2022] [Accepted: 06/21/2022] [Indexed: 12/02/2022] Open

Akindele AJ, Sowemimo A, Agunbiade FO, Sofidiya MO, Awodele O, Ade-Ademilua O, Orabueze I, Ishola IO, Ayolabi CI, Salu OB, Akinleye MO, Oreagba IA. Bioprospecting for Anti-COVID-19 Interventions From African Medicinal Plants: A Review. Nat Prod Commun 2022. [DOI: 10.1177/1934578x221096968] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022] Open

Affiliation(s)

Abidemi J. Akindele African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria
Abimbola Sowemimo African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria
Foluso O. Agunbiade African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Chemistry, Faculty of Science, University of Lagos, Lagos, Nigeria
Margaret O. Sofidiya African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria
Olufunsho Awodele African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria
Omobolanle Ade-Ademilua African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Botany, Faculty of Science, University of Lagos, Lagos, Nigeria
Ifeoma Orabueze African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria
Ismail O. Ishola African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria
Christianah I. Ayolabi African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Microbiology, Faculty of Science, University of Lagos, Lagos, Nigeria
Olumuyiwa B. Salu African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Medical Microbiology & Parasitology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria
Moshood O. Akinleye African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria
Ibrahim A. Oreagba African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS), University of Lagos (UNILAG), Lagos, Nigeria Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria

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Kashyap P, Kumar S, Riar CS, Jindal N, Baniwal P, Guiné RPF, Correia PMR, Mehra R, Kumar H. Recent Advances in Drumstick (Moringa oleifera) Leaves Bioactive Compounds: Composition, Health Benefits, Bioaccessibility, and Dietary Applications. Antioxidants (Basel) 2022;11:antiox11020402. [PMID: 35204283 PMCID: PMC8869219 DOI: 10.3390/antiox11020402] [Citation(s) in RCA: 58] [Impact Index Per Article: 19.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/16/2022] [Revised: 02/12/2022] [Accepted: 02/13/2022] [Indexed: 01/05/2023] Open

Alia F, Putri M, Anggraeni N, Syamsunarno MRAA. The Potency of Moringa oleifera Lam. as Protective Agent in Cardiac Damage and Vascular Dysfunction. Front Pharmacol 2022;12:724439. [PMID: 35140601 PMCID: PMC8818947 DOI: 10.3389/fphar.2021.724439] [Citation(s) in RCA: 20] [Impact Index Per Article: 6.7] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/13/2021] [Accepted: 12/14/2021] [Indexed: 12/25/2022] Open

Abdulhafiz F, Farhan Hanif Reduan M, Hamzah Z, Abdul Kari Z, Dawood MA, Mohammed A. Acute Oral Toxicity Assessment and Anti-hyperuricemic Activity of Alocasia longiloba Extracts on Sprague-Dawley Rats. Saudi J Biol Sci 2022;29:3184-3193. [PMID: 35844413 PMCID: PMC9280170 DOI: 10.1016/j.sjbs.2022.01.050] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/24/2021] [Revised: 12/11/2021] [Accepted: 01/22/2022] [Indexed: 12/19/2022] Open

Gupta P, Sonewane K, Chouhan S, Rajan M, Chauhan N, Rout O, Kumar A, Baghel G. Pharmacological, ethnomedicinal, and evidence-based comparative review of Moringa oleifera Lam. (Shigru) and its potential role in the management of malnutrition in tribal regions of India, especially Chhattisgarh. WORLD JOURNAL OF TRADITIONAL CHINESE MEDICINE 2022. [DOI: 10.4103/wjtcm.wjtcm_69_21] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022] Open

Santos ADS, Pimentel AL, Oliveira JVLD, Silva MTD, Silva FGC, Borges ALTF, Moura MAFBD, Silva SASD, Nascimento TGD. Phytochemical and pharmacological reports of the hypoglycemic activity of the Moringa oleifera extracts. RODRIGUÉSIA 2022. [DOI: 10.1590/2175-7860202273090] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]

Ghimire S, Subedi L, Acharya N, Gaire BP. Moringa oleifera: A Tree of Life as a Promising Medicinal Plant for Neurodegenerative Diseases. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2021;69:14358-14371. [PMID: 34843254 DOI: 10.1021/acs.jafc.1c04581] [Citation(s) in RCA: 19] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/13/2023]

Siddiqui F, Farooq AD, Kabir N, Fatima N, Abidi L, Faizi S. Toxicological assessment of Opuntia dillenii (Ker Gawl.) Haw. cladode methanol extract, fractions and its alpha pyrones: Opuntiol and opuntioside. JOURNAL OF ETHNOPHARMACOLOGY 2021;280:114409. [PMID: 34265378 DOI: 10.1016/j.jep.2021.114409] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/15/2021] [Revised: 07/08/2021] [Accepted: 07/09/2021] [Indexed: 06/13/2023]

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The edible plant Opuntia dillenii (Ker Gawl.) Haw. commonly known as Nagphana, belongs to the Cactaceae family. It is traditionally used to treat various ailments including inflammation, gastric ulcers, diabetes, hepatitis, asthma, whooping cough and intestinal spasm.

AIM OF THE STUDY

Despite its traditional use in various countries, detailed toxicological studies of O. dillenii cladode are few. Thus in the current study, toxicity of O. dillenii cladode derived methanol extract, fractions and its α-pyrones: opuntiol and opuntioside have been addressed.

METHODS

The test agents were assessed using both in vitro and in vivo toxicity assays. MTT on human embryonic kidney cell line (HEK-293), tryphan blue exclusion in rat neutrophils, Cytokinesis-B block micronucleus (CBMN) in human lymphocytes and genomic DNA fragmentation using agarose gel electrophoresis were performed. In acute toxicity test, mice orally received extract (5 g/kg) for 7 days followed by measurements of relative organ weight, biochemical (blood profile, liver and kidney function test) and histological studies (liver and kidney) were carried out. Rat bone marrow micronucleus genotoxicity assay was also conducted.

RESULTS

O. dillenii derived test agents were non-cytotoxic and had no effect on the integrity of DNA. Methanol extract (5 g/kg) orally administered in mice did not cause any significant change in relative organ weights, biochemical parameters and liver and kidney histology as compared to vehicle control. In parallel, extract did not stimulate micronuclei formation in rat bone marrow polychromatic erythrocytes.

CONCLUSION

These results led to conclude that edible O. dillenii extract is non-toxic via the oral route and appears to be non-cyto-, hepato-, nephro- or genotoxic, thereby supporting its safe traditional use against various ailments. Therefore, opuntiol and opuntioside may serve as lead compounds in designing new drug(s) derived from edible plants.

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Liu R, Liu J, Huang Q, Liu S, Jiang Y. Moringa oleifera: a systematic review of its botany, traditional uses, phytochemistry, pharmacology and toxicity. J Pharm Pharmacol 2021;74:296-320. [PMID: 34718669 DOI: 10.1093/jpp/rgab131] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/29/2021] [Accepted: 08/17/2021] [Indexed: 01/22/2023]

Aliyu A, Shaari MR, Ahmad Sayuti NS, Reduan FH, Sithambaram S, Mohamed Mustapha N, Shaari K, Hamzah HB. Moringa oleifera hydorethanolic leaf extract induced acute and sub-acute hepato-nephrotoxicity in female ICR-mice. Sci Prog 2021;104:368504211004272. [PMID: 34886737 PMCID: PMC10450773 DOI: 10.1177/00368504211004272] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]

Abstract

Moringa oleifera (M. oleifera) Lam belongs to the family Moringaceae. It is an important multipurpose tree that is largely distributed globally and has been used almost in every aspect of traditional medicine for the treatment of various illnesses including cancers, diabetes mellitus, asthma, arthritis, etc. This study investigated the effects of oral acute and sub-acute administration of M. oleifera hydroethanolic leaf extract (MOHE) in ICR-mice. Its major phenolic compounds were also determined. Ten (10) female, 8-week old mice were grouped into control and treatment groups for acute toxicity study. A dose of 2000 mg/kg MOHE was given once to the treatment group via oral gavage. However, for the sub-acute toxicity study, 25 mice were grouped into groups A (control), B (125 mg/kg), C (250 mg/kg), D (500 mg/kg) and E (1000 mg/kg). MOHE was given via oral gavage to groups B, C, D and E daily for 28 days. Group A received only distilled water. The mice were sacrificed at the end of the experiments and samples were collected for evaluation. The results of the chemical profiling of MOHE revealed the presence of glucomoringin, niaziminine, quercetin and kaempferol as the major compounds. The treated mice in the acute toxicity study were slightly anaemic and showed evidence of stress leukogram. Moreover, a slight increase in creatinine, significant increases in AST and CK, hepatic degeneration and necrosis, none-obstructive sinusoidal dilatation, renal tubular necrosis, interstitial nephritis and renal interstitial oedema were observed. It is concluded that the LD50 of MOHE is higher than 2000 mg/kg. However, oral administration of MOHE causes acute mild anaemia and moderate hepato-nephrotoxicity in ICR-mice. Its major phenolic compounds are glucomoringin, niaziminine, quercetin and kaempferol.

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NMR-Based Metabolomic Analyses to Identify the Effect of Harvesting Frequencies on the Leaf Metabolite Profile of a Moringa oleifera Cultivar Grown in an Open Hydroponic System. Molecules 2021;26:molecules26082298. [PMID: 33921119 PMCID: PMC8071497 DOI: 10.3390/molecules26082298] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/29/2020] [Revised: 02/26/2021] [Accepted: 03/03/2021] [Indexed: 11/16/2022] Open

Abstract

Moringa oleifera Lam. is one of the world’s most useful medicinal plants. Different parts of the M. oleifera tree contain a rich profile of important minerals, proteins, vitamins, and various important bioactive compounds. However, there are differences in the phytochemical composition of the medicinal plant’s raw materials due to seasonal variation, cultivation practices, and post-harvest processing. The main objective of this study was therefore to determine the effect of harvesting frequencies on selected bioactive compounds of a M. oleifera cultivar (PKM1) grown in a hydroponic system under a shade net structure. Three harvesting frequency treatments were applied in the study, with the plants harvested at every 30 days (high frequency), 60 days (intermediate frequency), and 90 days (low frequency) respectively. ¹H-NMR was used for data acquisition, and multivariate data analysis by means of principal component analysis (PCA), partial least square discriminatory analysis (PLS-DA), and orthogonal partial least square discriminatory analysis (OPLS-DA) were applied to determine the changes in the leaf metabolite profile, and also to identify the spectral features contributing to the separation of samples. Targeted metabolite analysis was used to match the NMR peaks of the compounds with the NMR chemical shifts of the contribution plot. The contribution plot showed that the increase in concentration of some compounds in aliphatic, sugar and aromatic regions contributed to the separation of the samples. The results revealed that intermediate and low harvesting frequencies resulted in a change in the leaf metabolite profile. Compounds such as chlorogenic acid, ferulic acid, vanillic acid, wogonin, esculetin, niazirin, and gamma-aminobutyric acid (GABA) showed an increase under intermediate and low harvesting frequencies. These results provide insight into the effect of harvesting frequencies on the metabolite profile and associated medicinal activity of M. oleifera.

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Peter EL, Nagendrappa PB, Hilonga S, Tuyiringire N, Ashuro E, Kaligirwa A, Sesaazi CD. Pharmacological reflection of plants traditionally used to manage diabetes mellitus in Tanzania. JOURNAL OF ETHNOPHARMACOLOGY 2021;269:113715. [PMID: 33358853 DOI: 10.1016/j.jep.2020.113715] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/19/2020] [Revised: 12/01/2020] [Accepted: 12/18/2020] [Indexed: 06/12/2023]

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The increasing national prevalence of diabetes mellitus (DM) and its complications have overstretched the health care system in Tanzania and influenced patients to use herbal medicines as alternative therapeutic strategies. Therefore, an urgent need exists to validate the safety and efficacy of plants used locally.

AIM OF THE STUDY

To identify plants used for the management of DM in Tanzania and analyses their pharmacological, phytochemistry, and safety evidence with a special focus on the mechanism of action.

METHODS

Researchers searched Medline, web of science, and Scopus for published articles. Also, specialized herbarium documents of Muhimbili Institute of traditional medicine were reviewed. Articles were assessed for relevance, quality, and taxonomical accuracy before being critically reviewed.

RESULTS

We identified 62 plant species used locally for DM management. Moringa oleifera Lam. and Cymbopogon citratus (D.C) stapf were the most mentioned. Fifty-four phytochemicals from 13 species had DM activities. These were mainly; polyphenolics, phytosterols, and triterpenoids. Extracts, fractions, and pure compounds from 18 species had in vitro antidiabetic activities of which 14 had α-glucosidase and α-amylase inhibition effects. The most studied -Momordica charantia L. increased; glucose uptake and adiponectin release in 3T3-L1 adipocytes, insulin secretion, insulin receptor substrate-1 (IRS-1), GLUT-4 translocation, and GLP-1 secretion; and inhibited protein tyrosine phosphatase 1 B (PTP1B). Preclinical studies reported 30 species that lower plasma glucose with molecular targets in the liver, skeletal muscles, adipose tissues, pancreases, and stomach. While three species; Aspilia mossambiscensis (Oliv.) Willd, Caesalpinia bonduc (L.) Roxb, and Phyllanthus amarus Schumach. & Thonn. had mild toxicity in animals, 33 had no report of their efficacy in DM management or toxicity.

CONCLUSION

Local communities in Tanzania use herbal medicine for the management of DM. However, only a fraction of such species has scientific evidence. A. mossambiscensis, C. bonduc., and P. amarus had mild toxicity in animals. Together, our findings call for future researches to focus on in vitro, in vivo, and phytochemical investigation of plant species for which their use in DM among the local communities in Tanzania have not been validated.

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Marvibaigi M, Hosseini SM, Amini N. Launaea acanthodes (Boiss) O. Kuntze mediates hepatic glucose metabolism and ameliorates impaired pancreatic function in streptozotocin-induced diabetic rats. JOURNAL OF ETHNOPHARMACOLOGY 2021;268:113577. [PMID: 33171271 DOI: 10.1016/j.jep.2020.113577] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/16/2020] [Revised: 10/26/2020] [Accepted: 11/06/2020] [Indexed: 06/11/2023]

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Launaea acanthodes (Boiss.) O. Kuntze is native to semiarid regions of central Iran, traditionally used in the treatment of numerous disorders including diabetes.

AIM OF THE STUDY

The current study aimed to explore hypoglycemic activity of Launaea acanthodes extract in streptozotocin-induced diabetic rats. Furthermore, gene expression study was carried out to examine expression levels of key glucose metabolism-related genes.

METHODS

For in vitro study, Folin-Ciocalteus, DPPH and aluminum chloride colorimetric assays were used to determine the total phenolic content, antioxidant capacity and total flavonoid content of extracts, respectively. For in vivo study, streptozotocin-induced diabetic Wistar rats were orally administered with metformin (50 mg/kg) and various doses of extracts (100, 200 and 400 mg/kg body weight) for 28 days. Fasting blood glucose, body weight, food and water intake were assessed during the course of treatment. At the end of the intervention, oral glucose tolerance test (OGTT), lipid profile and glycated hemoglobin (HbA1c) were evaluated. Furthermore, functional liver enzymes, oxidative stress markers and histopathology of pancreas were examined. Lastly, quantitative real time polymerase chain reaction (qRT-PCR) was applied to explore the mRNA levels of genes relevant to glucose metabolism in the pancreas and liver tissues of diabetic rats.

RESULTS

Based on the in vitro results, the hydroalcoholic extract revealed potential radical scavenging activity and contained highest amount of phenolic and flavonoid. The in vivo results demonstrated that the extract lowered fasting blood glucose level, increased the body weight, restored the alterations in the levels of water and food intake, attenuated HbA1c, improved lipid profile and ameliorated the OGTT in diabetic rats. The extract administration alleviated the histopathological changes in the pancreas, suppressed malondialdehyde (MDA) level and further restored attenuated levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in diabetic rats. Analysis of real time PCR data showed that extract administration reversed the expression levels of hepatic glucokinase (GK), phosphenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase). Meanwhile, the extract upregulated the expression level of glucose transporter-2 (GLUT-2) and pancreatic-duodenal homeobox (PDX-1) in diabetic rats.

CONCLUSION

Collectively, the results demonstrate that Launaea acanthodes hydroalcoholic extract exerts hypoglycemic effect possibly via regulating key enzymes of glucose metabolism and ameliorating pancreatic dysfunction through its antioxidant properties.

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Bailey-Shaw Y, Rodney S, Golding C, Foster S, Randle M, Watson C. The Potential Applications of Moringa oleifera Leaves in Functional Herbal Beverages: A Review of its Nutritional, Medicinal, and Toxicity Profiles. CURRENT NUTRITION & FOOD SCIENCE 2021. [DOI: 10.2174/1573401316999200802182952] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]

Wang F, Bao Y, Zhang C, Zhan L, Khan W, Siddiqua S, Ahmad S, Capanoglu E, Skalicka-Woźniak K, Zou L, Simal-Gandara J, Cao H, Weng Z, Shen X, Xiao J. Bioactive components and anti-diabetic properties of Moringa oleifera Lam. Crit Rev Food Sci Nutr 2021;62:3873-3897. [PMID: 33401950 DOI: 10.1080/10408398.2020.1870099] [Citation(s) in RCA: 25] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]

Affiliation(s)

Fang Wang College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China
Yifan Bao College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China
Chen Zhang College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China
Libin Zhan School of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, China
Washim Khan School of Pharmaceutical Education and Research, Bioactive Natural Product Laboratory, New Delhi, India
Sahifa Siddiqua School of Pharmaceutical Education and Research, Bioactive Natural Product Laboratory, New Delhi, India
Sayeed Ahmad School of Pharmaceutical Education and Research, Bioactive Natural Product Laboratory, New Delhi, India
Esra Capanoglu Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak, Istanbul, Turkey
Krystyna Skalicka-Woźniak Independent Laboratory of Natural Products Chemistry, Medical University of Lublin, Lublin, Poland
Liang Zou Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu, China
Jesus Simal-Gandara Nutrition and Bromatology Group, Department of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense, Spain
Hui Cao College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, China
Zebin Weng School of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, China
Xinchun Shen College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China
Jianbo Xiao Nutrition and Bromatology Group, Department of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo-Ourense Campus, Ourense, Spain College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, China International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang, China

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Abdellah RK, El-Beih NM, Morsy FA, Abdel-Wahhab KG, El-Hussieny EA. Moringa (Moringa oleifera) leaves aqueous extract enhances fibronectin type III domain-containing protein 5 gene expression and serum irisin liberation in an obesity model. EGYPTIAN PHARMACEUTICAL JOURNAL 2021;20:92-103. [DOI: 10.4103/epj.epj_50_20] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/03/2025]

Abstract Background Obesity, a risk agent for many chronic diseases, leads to increased mortality and poses one of the major public health problems. Objective This study aimed to investigate the thermogenic and antiobese efficiency of Moringa aqueous extract (MAE) on obese-modeled rats. Materials and methods Adult male rats (150–170 g) were randomly divided into four groups, with 10 animals each, as follows: (a) healthy rats served as control, (b) healthy rats administrated with MAE (400 mg/kg/day), (c) obese-modeled rats, and (d) obese-modeled rats treated with MAE. Results After 30 consecutive days of treatment, the obtained results declared that MAE possessed antiobesity, thermogenic, antilipidemic, and antiinflammatory potential. MAE succeeded significantly in reduction of the BMI and serum leptin level coupled with up-regulation of fibronectin type III domain-containing protein 5 gene mRNA expression and serum irisin level. It clearly increased serum paraoxonase-1 activity and improved lipid profile values. Moreover, it markedly reduced serum tumor necrosis factor α and increased antioxidant activity, which was achieved from the marked improvement in malondialdehyde, nitric oxide, catalase, superoxide dismutase, and glutathione values in cardio-hepatic tissues. These findings were confirmed by the regeneration of the hepatic histopathological structure. Conclusion MAE, as a food supplement, could play a beneficial role in management of obesity and restoring its complications. This could be exhibited through multiple pathways, mainly via upregulation of fibronectin type III domain-containing protein 5 gene expression and production of the soluble myokine ‘irisin,’ which is responsible for browning of white adipose tissue as well as increment of total body energy expenditure. Collapse

Moichela FT, Adefolaju GA, Henkel RR, Opuwari CS. Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro. Andrologia 2020;53:e13903. [PMID: 33225471 DOI: 10.1111/and.13903] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/07/2020] [Revised: 10/19/2020] [Accepted: 10/23/2020] [Indexed: 12/16/2022] Open

Saleem A, Saleem M, Akhtar MF, Ashraf Baig MMF, Rasul A. HPLC analysis, cytotoxicity, and safety study of Moringa oleifera Lam. (wild type) leaf extract. J Food Biochem 2020;44:e13400. [PMID: 32729119 DOI: 10.1111/jfbc.13400] [Citation(s) in RCA: 23] [Impact Index Per Article: 4.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/04/2020] [Revised: 06/10/2020] [Accepted: 07/03/2020] [Indexed: 01/01/2023]

Adharini WI, Nilamsari RV, Lestari ND, Widodo N, Rifa'i M. Immunomodulatory Effects of Formulation of Channa micropeltes and Moringa oleifera through Anti-Inflammatory Cytokines Regulation in Type 1 Diabetic Mice. PHARMACEUTICAL SCIENCES 2020. [DOI: 10.34172/ps.2020.43] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/09/2022] Open

Ajayi AM, Alabi AO, Adebesin A, Ben-Azu B, Umukoro S. Aqueous extract of dry powder blend of seeds and leaves of Picralima nitida (Stapf) T. & H. Durand reduce pain and inflammation in animal models. J Basic Clin Physiol Pharmacol 2020;32:97-107. [PMID: 32598312 DOI: 10.1515/jbcpp-2019-0212] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/09/2019] [Accepted: 04/09/2020] [Indexed: 06/11/2023]

Subacute Oral Administration of Clinacanthus nutans Ethanolic Leaf Extract Induced Liver and Kidney Toxicities in ICR Mice. Molecules 2020;25:molecules25112631. [PMID: 32517000 PMCID: PMC7325574 DOI: 10.3390/molecules25112631] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/27/2020] [Revised: 04/27/2020] [Accepted: 04/27/2020] [Indexed: 11/16/2022] Open

Medicinal plants used by traditional medicine practitioners to boost the immune system in people living with HIV/AIDS in Uganda. Eur J Integr Med 2020. [DOI: 10.1016/j.eujim.2019.101011] [Citation(s) in RCA: 19] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]

Popoola JO, Aworunse OS, Oyesola OL, Akinnola OO, Obembe OO. A systematic review of pharmacological activities and safety of Moringa oleifera. JOURNAL OF HERBMED PHARMACOLOGY 2020. [DOI: 10.34172/jhp.2020.24] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/29/2022] Open

Alabi AO, Ajayi AM, Omorogbe O, Umukoro S. Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava. CLINICAL PHYTOSCIENCE 2019. [DOI: 10.1186/s40816-019-0130-2] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open

Abstract Abstract Background To investigate the antinociceptive and anti-inflammatory activities of aqueous extract of a blended mixture of dried leaves of Ocimum gratissimum and Psidium guajava, a traditional analgesic drug polyherbal (TADP) used as a remedy for pain-related conditions. Methods Antinociceptive activity of TADP (100, 200 and 400 mg/kg) was evaluated in the hot plate test and acetic acid-induced nociception in mice while the anti-inflammatory was evaluated in carrageenan-induced paw oedema in rats. Levels of nitrite, myeloperoxidase, glutathione and malondialdehyde were assayed in carrageenan-induced paw tissue. Results TADP (200 and 400 mg/kg) significantly prolong the latency time in the hot-plate test. TADP (100–400 mg/kg) produced a dose-dependent significant inhibition of the acetic-acid induced abdominal constriction. The antinociceptive activity of TADP in the presence of naloxone and atropine was not reversed whereas yohimbine and glibenclamide significantly reversed it. TADP (100, 200 and 400 mg/kg) significantly reduced the swelling in the carrageenan-induced oedema model and also produced a reduction in the nitrite and myeloperoxidase level. TADP (400 mg/kg) significantly reduced malondialdehyde concentration and increase glutathione level in the carrageenan-induced rat paw. TADP significantly decrease the number of cellular infiltrates in the histopathological assessment. Conclusion These results indicate that polyherbal product containing blended leaves of Ocimum gratissimum and Psidium guajava possess antinociceptive and anti-inflammatory properties, hence represents a promising alternative remedy in inflammation-induced pain. Collapse

Fantoukh OI, Albadry MA, Parveen A, Hawwal MF, Majrashi T, Ali Z, Khan SI, Chittiboyina AG, Khan IA. Isolation, synthesis, and drug interaction potential of secondary metabolites derived from the leaves of miracle tree (Moringa oleifera) against CYP3A4 and CYP2D6 isozymes. PHYTOMEDICINE : INTERNATIONAL JOURNAL OF PHYTOTHERAPY AND PHYTOPHARMACOLOGY 2019;60:153010. [PMID: 31301970 DOI: 10.1016/j.phymed.2019.153010] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/24/2019] [Revised: 06/21/2019] [Accepted: 07/01/2019] [Indexed: 06/10/2023]

Abstract

BACKGROUND

Moringa oleifera Lam. is known as a drumstick tree that is widely cultivated in various subtropical and tropical provinces. Previous studies indicated that both aqueous and methanolic extracts of M. oleifera leaves have potent inhibitory effects on two major drug metabolizing Cytochrome P450 enzymes, namely, CYP3A4 and CYP2D6.

PURPOSE

The current study was aimed to isolate the secondary metabolites from M. oleifera and investigate their cytotoxicity and inhibitory effects on CYP3A4 and CYP2D6 to assess their herb-drug interaction (HDI) potential.

METHODS

Chemical structure elucidation was achieved by interpreting the spectroscopic data (UV, IR, 1D, and 2D NMR experiments), confirming by HR-ESI-MS, and comparing with the previously reported data in the literature. All the isolates were evaluated for their cytotoxicity against a panel of cell lines (SK-MEL, KB, BT-549, SK-OV-3, VERO, LLC-PK1, and HepG2) and inhibition of two principal CYP isozymes (CYP3A4 and CYP2D6).

RESULTS

Phytochemical investigation of M. oleifera leaves resulted in the isolation and characterization of one new compound, namely omoringone (1), along with twelve known secondary metabolites (2-13) belonging to several chemical classes including flavonoids, terpenoids, lignans, and phenylalkanoids. A plausible biosynthetic pathway for compound 1 was provided. Because of the low isolation yield and limited supply, omoringone (1) and niazirin (12) were successively synthesized. No cytotoxicity was observed on any of the tested cell lines up to 50 µM. The extract exhibited an inhibitory effect on CYP3A4 isoform (IC₅₀ = 52.5 ± 2.5 µg/ml). Among the isolates, 1-4 and 7-9 inhibited CYP3A4 with the IC₅₀ values ranging from 41.5 to 100 µM with no remarkable effect on CYP2D6 isozyme.

CONCLUSION

This work aided in ascertaining components of M. oleifera contributing to CYP3A4 inhibition exhibited by the extract using an in vitro assay. Nonetheless, further studies are warranted to determine the bioavailability of the phytochemicals and extrapolate these findings in more physiologically relevant conditions to further establish the clinical relevance of in vitro observations.

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Affiliation(s)

Omer I Fantoukh National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 4545, Saudi Arabia.
Mohamed A Albadry National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA
Abidah Parveen National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Department of Pharmaceutical Sciences, Abbottabad University of Science & Technology, Havelian, KPK, Pakistan
Mohammed F Hawwal National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 4545, Saudi Arabia
Taghreed Majrashi National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA
Zulfiqar Ali National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA
Shabana I Khan National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA
Amar G Chittiboyina National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA
Ikhlas A Khan National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS 38677, USA; Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA.

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