Basic Study
Copyright ©The Author(s) 2016.
World J Hepatol. Oct 18, 2016; 8(29): 1212-1221
Published online Oct 18, 2016. doi: 10.4254/wjh.v8.i29.1212
Table 1 Demographics and characteristics of participants
n%
Gender (n = 101)
Male3938.6
Female6261.4
Age (yr) (n = 95)
19-293840.0
30-391920.0
40-491717.9
50-591111.6
60-6966.3
70-7922.1
80-8922.1
Education (n = 99)
Illiterate33.0
Primary School Education33.0
Secondary School Graduate99.1
High School Education1010.1
University Undergraduate Level5050.5
Others2424.2
Occupation (n = 99)
Student1515.2
Employed7474.7
Unemployed77.1
Retired33
Table 2 The observed and estimated killer immunoglobulin receptor gene frequencies in the study participants
Inhibitory KIR
Non-inhibitory KIR
Pseudogene
2DL12DL22DL32DL42DL53DL13DL23DL32DS12DS22DS32DS42DS53DS12DP13DP*001/002/0043DP1*003
OF995684100429610010040.655.446.59534.741.69917100
KLF0.90.340.610.240.8110.230.330.270.780.190.240.911
Table 3 Killer immunoglobulin receptor gene frequencies among study participants vaccinated against hepatitis B virus (protected and non-protected by hepatitis B vaccine) n (%)
KIR genesAnti-HBsAg < 10 mIU/mL (n = 22)Anti-HBsAg ≥ 10 mIU/mL (n = 52)Test1OR (95%CI)P value
2DL214 (63.60)27 (51.90)0.8580.62 (0.22-1.72)0.446
2DL318 (81.80)45 (86.40)FET1.43 (0.37-5.48)0.723
2DL5A8 (36.40)13 (25.00)0.9820.58 (0.20-1.70)0.4
2DL5B5 (22.70)19 (36.50)1.3461.97 (0.62-6.16)0.289
2DS111 (50.00)19 (36.50)1.1620.58 (0.21-1.58)0.31
2DS214 (63.60)26 (50.00)1.1570.57 (0.21-1.59)0.318
2DS39 (40.90)23 (44.20)0.0691.15 (0.42-3.15)0.804
2DS4*001/0028 (36.40)20 (38.40)0.0291.09 (0.39-3.072)1.00
2DS4*003/00719 (86.40)41 (78.85)FET0.59 (0.15-2.36)0.534
2DS510 (45.50)17 (32.60)1.0870.58 (0.21-1.62)0.428
3DS111 (50.00)21 (40.40)0.5820.68 (0.25-1.85)0.608
3DP*001/002/0046 (27.30)9 (17.30)FET0.56 (0.17-1.82)0.355
Table 4 Killer immunoglobulin receptor gene frequencies among study participants vaccinated against hepatitis B virus as compared to non-vaccinated subjects n (%)
KIR genesVaccinated (n = 74)Non-vaccinated (n = 25)Test1OR (95%CI)P value
2DL241 (55.40)15 (60.00)0.1610.83 (0.33-2.082)0.436
2DL363 (85.10)20 (80.00)0.3641.43 (0.44-4.62)0.374
2DL5A21 (28.40)4 (16.00)1.5172.08 (0.64-6.79)0.168
2DL5B24 (32.40)5 (20.00)1.3951.92 (0.64-5.73)0.178
2DS130 (40.50)10 (40.00)0.0021.02 (0.41-2.58)0.577
2DS240 (54.10)15 (60.00)0.2680.78 (0.31-1.97)0.39
2DS332 (43.20)14 (56.00)1.2230.60 (0.24-1.49)0.191
2DS4*001/00228 (37.80)11 (44.00)0.2970.77 (0.31-1.94)0.376
2DS4*003/00760 (81.10)22 (88.00)FET0.58 (0.15-2.23)0.324
2DS527 (36.50)7 (28.00)0.5971.47 (0.55-3.99)0.302
3DS132 (43.20)9 (36.00)0.2461.35 (0.53-3.46)0.401
3DP*001/002/00415 (20.30)2 (8.00)FET2.92 (1.62-13.80)0.134
Table 5 The relationship between AA, AB and BB genotypes and the expression of killer immunoglobulin receptor genes among the study participants n (%)
KIR genesAA (n = 44)AB (n = 41)BB (n = 16)Test1P value
2DL20 (0.00)41 (100.00)16 (100.00)1010.00a
2DL344 (100.00)41 (100.00)0 (0.00)1010.00a
2DL5A10 (22.70)10 (22.70)5 (31.25)FET0.782
2DL5B1 (2.27)20 (24.39)8 (50.00)FET0.00a
2DS116 (36.30)18 (43.90)7 (43.75)0.5790.749
2DS20 (0.00)40 (97.50)16 (100.00)97.0510.00a
2DS33 (6.80)30 (73.17)14 (87.50)50.380.00a
2DS4*001/00216 (36.40)19 (37.50)6 (37.50)0.9520.621
2DS4*003/00738 (86.40)31 (75.60)14 (87.50)FET0.392
2DS514 (31.80)16 (39.00)5 (31.30)0.5840.747
3DS118 (40.90)18 (43.90)6 (40.00)0.1070.948
3DP*001/002/0042 (4.50)10 (24.40)5 (31.30)FET0.0006a
Table 6 Killer immunoglobulin receptor gene expression and levels of anti-HBs among the vaccinated study participants n (%)
KIR genesGroup I1 (n = 36)Group II2 (n = 16)Group III3 (n = 15)Group IV4 (n = 7)Test5P value
2DL217 (47.20)10 (62.50)11 (73.30)3 (42.90)FET0.362
2DL331 (86.10)14 (87.50)12 (80.00)6 (85.70)FET0.824
2DL5A9 (25.00)4 (25.00)4 (26.70)4 (57.10)FET0.987
2DL5B9 (25.00)10 (62.50)4 (26.70)1 (14.30)FET0.0236
2DS111 (30.60)8 (50.00)7 (46.70)4 (57.10)FET0.530
2DS216 (44.40)10 (62.50)11 (73.30)3 (42.90)FET0.270
2DS312 (33.30)11 (68.80)8 (53.30)1 (14.30)5.010.060
2DS4*001/00215 (41.70)5 (31.30)6 (40.00)2 (28.60)0.5680.920
2DS4*003/00728 (77.80)13 (81.30)13 (86.70)6 (85.70)FET0.860
2DS511 (30.60)6 (37.50)6 (40.00)3 (42.90)0.8460.850
3DS114 (38.90)7 (43.80)7 (46.70)6 (85.70)FET0.960
2DL436 (100.00)16 (100.00)15 (100.00)4 (57.10)0.2580.970
3DP*001/002/0048 (22.20)1 (6.30)4 (26.70)1 (14.30)FET0.420