Effect of dichloromethylene diphosphonate on liver regeneration following thioacetamide-induced necrosis in rats

Table 1 Quantitative analysis of the DNA ploidy in hepatocytes of adult rats following different treatments

Group	Hypodiploid	Diploid	S1 Phase	Tetraploid	S2 Phase	Octoploid
	(< 2N)	(2N)	(2N→4N)	(4N)	(4N→8N)	(8N)
Control	0.98	12.3	2	75.31	2.48	3.97
Control DMDP	0.74	18.86	0.98	71.09	5.6	2.67
TA 24 h	1.61	41.74a	8.57a	39.0ª	7.82ª	0.9
TA-DMDP 24 h	2.01	25.45ac	18.07ac	49.20ac	3.92	0.76
TA 48 h	1.59	52.87a	11.95a	22.86a	10.18a	0.2
TA-DMDP 48 h	2.35	42.77a	14.92a	28.0a	10.25ª	1.7
TA 72 h	3.78a	47.61a	7.21a	35.6ª	4.29ª	1.12
TA-DMDP 72 h	2.65ac	45.99a	1.44c	41.71ª	5.98	1.83

The values are expressed as the percentage of DNA in the following: hypodiploid population (< 2N); diploid population, 2N (cells in G0-G1); tetraploid population, 4N (cells in G2 + M); octaploid population, 8N, and cells synthesizing DNA, S1 phase (from G1-G2, 2N→4N) and S2 phase (from 4N→8N). Data are expressed as the means of four experimental observations (four rats) ± SD,

^aP < 0.05 vs the untreated control group;

^cP < 0.05 vs changes due to dichloromethylene diphosphonate (DMDP). TA: Thioacetamide.