HBV vaccine efficacy and detection and genotyping of vaccineé asymptomatic breakthrough HBV infection in Egypt

Table 1 Percent of positive cases, for HBs antibodies (IgG) by ELISA in the three studied population

	Group IChildren (2- < 4 years)N=200	Group IIChildren (4-13 years)N =400	Group IIIAdults (20-47 years)N =100	TotalNoN =700
Anti-HBs IgG	n (%)	n (%)	n (%)	n (%)
High +ve samples (10-99.9 mIU/mL)	130 (65)	82 (20.5)	45 (45)	257(36.7)
Low +ve samples (<10 mIU/mL)	56 (28)	238 (59.5)	34 (34)	328(46.9)
Negative samples (Non-responders)	14 (7 )	80 (20 )	21 (21 )	115 (16.4)
Total positive samples	186 (93)	320 (80)	79 (79)	585 (83.6)

Table 2 Frequency of HBs antigen by ELISA and AxSYM among r-HBsAg vaccine responders and non responders

	Anti-HBs IgG antibody level	Samples tested	HBs Ag
			(ELISA)	(AxSYM)
		n	Positive n (%)	Positive n ( %)
Group I Children (2-<4 years)	Low	27	0	2 (7.4)
	Negative	8	0	3 (37.5)
	High	20	0	0
Group II Children (4-13 years)	Low	178	4 (2.25)	17 (9.55)
	Negative	53	1 (1.88)	11 (20.8)
	High	14	0	1a (7.14)
Group III Adults (20-47 years)	Low	32	2 (6.25)	2 (6.25)
	Negative	21	2 (9.52)	5 (23.8)
	High	7	0	0
Total		360	9 (2.5)	41b (11.39)

^aExamination of 41 high anti-HBs IgG antibodies serum samples demonstrated that all markers tested were negative, except for one sample that was positive for HBsAg by AxSYM;

^bThe number of positive samples for HBs antigen were 41 samples by AxSYM in comparison with 9 samples.

Table 3 Frequency of HBV-DNA by nested Multiplex PCR, HBc and HBe anti bodies among r-HBsAg vaccine responders and non responders

Age group	anti-HBs IgG level	Number of serum samplesTested	HBV-DNA by nestedMultiplex PCR n (%)	HBc antibodyIgG n (%)	HBe IgG antibodyn (%)
Group I Children (2-<4 years)	Low	27	0	0	0
	Negative	8	0	0	0
	High	20	0	0	0
Group II Children (4-13 years)	Low	178	4 (2.5)	2 (1.2)	0
	Negative	53	14 (26.4)	1 (1.9)	1 (1.9)
	High	14	0	0	0
Group III Adults (20-47 years)	Low	32	2a (6.25)	1 (3.1)	1 (3.1)
	Negative	21	2a (9.52)	1 (4.8)	1 (4.8)
	High	7	0	0	0
Total		360	22 (6.11)	5 (1.38)	3 (0.83)

^aThese four +ve sera are also +ve for HBs antigen &HBcAb detected by ELISA.