Prognostic role of ring finger and WD repeat domain 3 and immune cell infiltration in hepatocellular carcinoma

Table 1 Detailed statistical results of differential expression of ring finger and WD repeat domain 3 in hepatocellular carcinoma and normal tissues

Gene	Group	n	Minimum	Maximum	Median	IQR	Lower quartile	Upper quartile	Mean	SD	SE
RFWD3	Normal	50	0.504	1.504	0.98	0.251	0.883	1.133	1.013	0.208	0.029
	Tumor	374	0.62	3.5	1.544	0.755	1.245	2	1.647	0.559	0.029
	Normal	50	0.504	1.504	0.98	0.251	0.883	1.133	1.013	0.208	0.029
	Tumor	50	0.707	2.939	1.577	0.716	1.204	1.92	1.578	0.521	0.074

RFWD3: Ring finger and WD repeat domain 3; IQR: Interquartile range; SD: Standard deviation; SE: Standard error.

Table 2 Detailed information on the statistical correlation between ring finger and WD repeat domain 3 expression and immune cell infiltration

Gene	Immune cell type	Pearson’s correlation coefficient	Pearson’s P value	Spearman’s correlation coefficient	Spearman’s P value
RFWD3	Th2 cells	0.499	< 0.001	0.501	< 0.001
	Th cells	0.434	< 0.001	0.436	< 0.001
	Cytotoxic cells	-0.304	< 0.001	-0.314	< 0.001
	DCs	-0.281	< 0.001	-0.304	< 0.001
	pDCs	-0.261	< 0.001	-0.261	< 0.001
	Neutrophils	-0.210	< 0.001	-0.214	< 0.001
	TFH cells	0.226	< 0.001	0.213	< 0.001
	Tcm cells	0.187	< 0.001	0.164	0.002
	Tgd cells	-0.105	0.043	-0.142	0.006
	Tregs	-0.155	0.003	-0.121	0.019
	aDCs	0.141	0.006	0.114	0.027
	NK CD56bright cells	0.128	0.013	0.112	0.031
	Th17 cells	-0.170	< 0.001	-0.110	0.033
	Eosinophils	0.077	0.135	0.106	0.041
	Macrophages	0.096	0.063	0.071	0.171
	Th1 cells	0.090	0.081	0.064	0.214
	iDCs	-0.034	0.507	-0.061	0.241
	Mast cells	-0.053	0.309	-0.060	0.247
	CD8 T cells	-0.047	0.368	-0.058	0.260
	T cells	-0.013	0.796	-0.033	0.522
	Tem cells	0.085	0.100	0.020	0.704
	B cells	0.033	0.525	0.017	0.744
	NK cells	0.035	0.494	-0.012	0.810
	NK CD56dim cells	0.020	0.699	-0.001	0.979

aDCs: Activated dendritic cells; DCs: Dendritic cells; iDCs: Immature dendritic cells; NK: Natural killer; pDCs: Plasmacytoid dendritic cells; RFWD3: Ring finger and WD repeat domain 3; Tcm: T central memory; Tem: T effector memory; TFH: T follicular helper; Tgd: T gamma delta.

Table 3 Univariate Cox regression analysis in hepatocellular carcinoma

Characteristics	Total, n	Univariate analysis
		Hazard ratio (95%CI)	P value
Pathologic stage	349
I	173
II	86	1.417 (0.868-2.312)	0.164
III	85	2.734 (1.792-4.172)	< 0.001
IV	5	5.597 (1.726-18.148)	0.004
Child-Pugh grade	240
A	218
B	21	1.595 (0.757-3.361)	0.219
C	1	2.138 (0.294-15.544)	0.453
Fibrosis Ishak score	214
0	75
1/2	31	0.935 (0.437-2.002)	0.864
3/4	28	0.698 (0.288-1.695)	0.428
5/6	80	0.737 (0.410-1.325)	0.308
Histologic grade	368
G1	55
G2	178	1.162 (0.686-1.969)	0.576
G3	123	1.185 (0.683-2.057)	0.545
G4	12	1.681 (0.621-4.549)	0.307
RFWD3	373	1.557 (1.148-2.110)	0.004

CI: Confidence interval; RFWD3: Ring finger and WD repeat domain 3.