Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution

Figure 3 Hepatocyte functionality before and after freezing. Activity of the major cytochrome P450 enzymes; CYP1A2, CYP2C9, CYP3A7, CYP3A4, CYP2C19, CYP2D6 for the fresh hepatocytes (FRESH) compared to hepatocytes cryopreserved using either the STEM-CELLBANKER protocol (CB) or the standard dimethylsulfoxide in the University-of-Wisconsin solution protocol. The standard deviation exceeded the mean in many cases, illustrating the high within-groups variability. Data is presented as luminescence (LCU) per minute per DNA in nanograms. CYPs: Cytochrome P450 enzymes.