Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution

doi:10.4254/wjh.v4.i5.176

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May 27, 2012 (publication date) through Apr 23, 2024

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. May 27, 2012; 4(5): 176-183
Published online May 27, 2012. doi: 10.4254/wjh.v4.i5.176

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Figure 1 Hepatocyte viability before and after freezing. Viability in the three groups: fresh hepatocytes (FRESH) and hepatocytes frozen using either the STEM-CELLBANKER protocol (CB) or the standard dimethylsulfoxide in the University-of-Wisconsin solution (DMSO-UW) protocol using both the trypan blue exclusion and the Live/Dead Assay (LDA) methods. There was a significant difference in viability between CB and DMSO using the two-way analysis of variance test (P < 0.05). TB: Trypan blue.

Citation: Saliem M, Holm F, Tengzelius RB, Jorns C, Nilsson LM, Ericzon BG, Ellis E, Hovatta O. Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution. World J Hepatol 2012; 4(5): 176-183
URL: https://www.wjgnet.com/1948-5182/full/v4/i5/176.htm
DOI: https://dx.doi.org/10.4254/wjh.v4.i5.176