Copyright ©2010 Baishideng.
World J Hepatol. Apr 27, 2010; 2(4): 151-161
Published online Apr 27, 2010. doi: 10.4254/wjh.v2.i4.151
Figure 2
Figure 2 Measurement of fucosylation-related tumor markers in gastrointestinal cancer. A: The sera of patients with liver diseases were electrophoresed on an LCA agarose gel, followed by reaction with anti-AFP antibody. Since LCA specifically binds to fucosylated oligosaccharides on AFP, fucosylated AFP runs slowly on an LCA agarose gel; B: Since IgG has a fucosylated oligosaccharide in its Fc portion, a Fab fragment of anti-human haptoglobin IgG was coated on the bottom of a 96-well ELISA plate. After the sera of patients had been loaded into individual wells, the reaction with biotinylated AAL was performed to detect specifically fucosylated haptoglobin. Peroxidase-conjugated avidin and 3,3’,5,5’ tetramethylbenzisine were used for development.