Beneficial effects of losartan or telmisartan on the local hepatic renin-angiotensin system to counter obesity in an experimental model

Figure 1 Body mass (A) and systolic blood pressure (B) pre-treatment (10^th week) and end of treatment (15^th week). Data are the mean ± SD, n = 5 for each group. Differences between the groups were tested with one-way ANOVA and Holm–Sidak post hoc test (P < 0.05) compared to the C group (a), HF group (c) and HFL group (e). C: Control group; HF: High-fat diet; HFL: HF diet treated with losartan; HFT: HF diet treated with telmisartan.

Figure 2 Angiotensin type-1 receptor (A) and PLIN 2 (B) assessed by immunofluorescence (green) in the liver. Scale bars correspond to 50 µm, same magnification for all immunoreactions (40 x). C: Control group; HF: High-fat diet; HFL: HF diet treated with losartan; HFT: HF diet treated with telmisartan.

Figure 3 Local gene expression of components of the classical ACE1/AT1r axis in the liver: Renin (A), ACE1 (B), AT1r (C) and AT2r (B). B-Actin was used as an endogenous control to normalize the expression of the selected genes. Data are the means ± SD, n = 5 for each group. Differences between the groups were tested with one-way ANOVA and post hoc test of Holm–Sidak (P < 0.05) when compared with the C group (a) and HF group (c). ACE1: Angiotensin-converting enzyme 1; AT1r: Angiotensin type-1 receptor; AT2r: Angiotensin type-2 receptor; C: Control group; HF: High-fat diet; HFL: HF diet treated with losartan; HFT: HF diet treated with telmisartan.

Figure 4 Local gene expression of the ACE2/MASr axis in the liver: ACE2 (A) and rMAS (B). B-Actin was used as an endogenous control to normalize the expression of the selected genes. Data are the means ± SD, n = 5 for each group. Differences between the groups were tested with one-way ANOVA and post hoc test of Holm–Sidak (P < 0.05) compared to the C group (a), HF group (c) and HFL group (e). ACE2: Angiotensin-converting enzyme 2; rMAS: MAS receptor; C: Control group; HF: High-fat diet; HFL: HF diet treated with losartan; HFT: HF diet treated with telmisartan.