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Nunes PF, da Cruz Coelho E, da Silva JR, da Silva Costa CC, Sampaio RMA, Frade PCR, Ponteira NA, da Cruz SS, Seabra AD, Carneiro DM, Burbano RMR, Martins LC. Hepatitis C and Human Pegivirus Coinfection in Patients with Chronic Hepatitis C from the Brazilian Amazon Region: Prevalence, Genotypes and Clinical Data. Viruses 2023; 15:1892. [PMID: 37766298 PMCID: PMC10536978 DOI: 10.3390/v15091892] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/20/2023] [Revised: 08/08/2023] [Accepted: 08/20/2023] [Indexed: 09/29/2023] Open
Abstract
Coinfection of HPgV-1 with hepatitis C virus (HCV) is common due to shared modes of transmission, with a prevalence of HPgV-1 viremia of approximately 20% among individuals with chronic HCV infection. The aim of the present study was to estimate the prevalence of HPgV-1 RNA and circulating genotypes in patients with hepatitis C from a health service located in the city of Belém, in the state of Pará, Northern Brazil. A total of 147 samples were included in the study from February to December 2019. Among the participants, 72.1% (106/147) were monoinfected with HCV, with detectable HCV viral RNA, and 27.9% (41/147) were coinfected with HCV/HPgV-1. The most frequently found genotypes were HPgV-1 genotypes 1 and 2 (36.6% and 63.4%), respectively. While for HCV there was a predominance of genotypes 1 and 3 (58.5% and 41.5%). No significant differences were found when comparing any risk, sociodemographic, or clinical factors between groups. Also, there was no statistically significant difference when relating the viral genotypes of both agents. This study indicated that the prevalence of infection by HPgV-1 is high in HCV carriers in Belém, Pará, and probably does not change the clinical course of HCV infection, however, further studies are still needed.
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Affiliation(s)
- Patrícia Ferreira Nunes
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
| | - Evelen da Cruz Coelho
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
| | - Joseane Rodrigues da Silva
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
| | - Camila Carla da Silva Costa
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
| | - Regiane Miranda Arnund Sampaio
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
| | - Paula Cristina Rodrigues Frade
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
| | - Nagib Abdon Ponteira
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
| | - Samara Silveira da Cruz
- Laboratório de Biologia Molecular, Hospital Ophir Loyola, Belém 66063-240, PA, Brazil; (S.S.d.C.); (A.D.S.); (D.M.C.); (R.M.R.B.)
| | - Aline Damasceno Seabra
- Laboratório de Biologia Molecular, Hospital Ophir Loyola, Belém 66063-240, PA, Brazil; (S.S.d.C.); (A.D.S.); (D.M.C.); (R.M.R.B.)
| | - Debora Monteiro Carneiro
- Laboratório de Biologia Molecular, Hospital Ophir Loyola, Belém 66063-240, PA, Brazil; (S.S.d.C.); (A.D.S.); (D.M.C.); (R.M.R.B.)
| | - Rommel Mario Rodriguez Burbano
- Laboratório de Biologia Molecular, Hospital Ophir Loyola, Belém 66063-240, PA, Brazil; (S.S.d.C.); (A.D.S.); (D.M.C.); (R.M.R.B.)
| | - Luisa Caricio Martins
- Núcleo de Medicina Tropical, Laboratório de Patologia Clínica das Doenças Tropicais, Universidade Federal do Pará, Belém 66055-240, PA, Brazil; (E.d.C.C.); (J.R.d.S.); (C.C.d.S.C.); (R.M.A.S.); (P.C.R.F.); (N.A.P.); (L.C.M.)
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Niama RF, Mayengue PI, Nsoukoula BCM, Koukouikila-Kossounda F, Badzi CN, Mandiangou AF, Louzolo I, Fila-Fila GPU, Dossou-Yovo LR, Angounda MB. Genetic variability of human pegivirus type 1 (HPgV-1) among Congolese co-infected with hepatitis C virus in Brazzaville, Congo. IJID REGIONS 2023; 7:191-192. [PMID: 37123381 PMCID: PMC10131043 DOI: 10.1016/j.ijregi.2023.03.005] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 01/09/2023] [Revised: 03/13/2023] [Accepted: 03/13/2023] [Indexed: 05/02/2023]
Abstract
The aim of this study was to determine the rate of human pegivirus type 1 (HPgV-1) and hepatitis C virus (HCV) co-infection, and the genotype distribution of HPgV-1 among patients with chronic hepatitis C and blood donors in Brazzaville. Two groups of patients in Brazzaville were recruited: blood donors (n = 35) and individuals with chronic hepatitis C (n = 73). The overall positivity rate of HPgV-1 was 4.63%: 2.86% in blood donors and 5.48% in chronic hepatitis C patients. Phylogenetic analysis showed that all samples were genotype 1. However, studies with a larger sample size are needed to estimate the true burden of HPgV-1 infection in the country and to confirm the distribution of genotypes in the general population.
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Affiliation(s)
- Roch Fabien Niama
- Laboratoire National de Santé Publique, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
- Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo
- Corresponding author: Fabien Roch Niama, Laboratoire National de Santé Publique (LNSP), Unité de Biologie Moléculaire, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, République du Congo.
| | - Pembe Issamou Mayengue
- Laboratoire National de Santé Publique, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
- Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo
| | | | - Félix Koukouikila-Kossounda
- Laboratoire National de Santé Publique, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
- Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo
| | - Cynthia Nkoua Badzi
- Laboratoire National de Santé Publique, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
| | - Aldi Fred Mandiangou
- Laboratoire National de Santé Publique, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
- Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo
| | - Igor Louzolo
- Laboratoire National de Santé Publique, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
| | | | - Louis Régis Dossou-Yovo
- Laboratoire National de Santé Publique, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
- Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo
| | - Monic Brunel Angounda
- Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo
- Centre National de Transfusion Sanguine, 120 avenue du Général Charles de Gaulle, BP 120, Brazzaville, Congo
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Stapleton JT. Human Pegivirus Type 1: A Common Human Virus That Is Beneficial in Immune-Mediated Disease? Front Immunol 2022; 13:887760. [PMID: 35707535 PMCID: PMC9190258 DOI: 10.3389/fimmu.2022.887760] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/02/2022] [Accepted: 05/03/2022] [Indexed: 12/25/2022] Open
Abstract
Two groups identified a novel human flavivirus in the mid-1990s. One group named the virus hepatitis G virus (HGV) and the other named it GB Virus type C (GBV-C). Sequence analyses found these two isolates to be the same virus, and subsequent studies found that the virus does not cause hepatitis despite sharing genome organization with hepatitis C virus. Although HGV/GBV-C infection is common and may cause persistent infection in humans, the virus does not appear to directly cause any other known disease state. Thus, the virus was renamed “human pegivirus 1” (HPgV-1) for “persistent G” virus. HPgV-1 is found primarily in lymphocytes and not hepatocytes, and several studies found HPgV-1 infection associated with prolonged survival in people living with HIV. Co-infection of human lymphocytes with HPgV-1 and HIV inhibits HIV replication. Although three viral proteins directly inhibit HIV replication in vitro, the major effects of HPgV-1 leading to reduced HIV-related mortality appear to result from a global reduction in immune activation. HPgV-1 specifically interferes with T cell receptor signaling (TCR) by reducing proximal activation of the lymphocyte specific Src kinase LCK. Although TCR signaling is reduced, T cell activation is not abolished and with sufficient stimulus, T cell functions are enabled. Consequently, HPgV-1 is not associated with immune suppression. The HPgV-1 immunomodulatory effects are associated with beneficial outcomes in other diseases including Ebola virus infection and possibly graft-versus-host-disease following stem cell transplantation. Better understanding of HPgV-1 immune escape and mechanisms of inflammation may identify novel therapies for immune-based diseases.
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Affiliation(s)
- Jack T. Stapleton
- Medicine Service, Iowa City Veterans Administration Healthcare, Iowa City, IA, United States
- Departments of Internal Medicine, Microbiology & Immunology, University of Iowa, Iowa City, IA, United States
- *Correspondence: Jack T. Stapleton,
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Cebriá-Mendoza M, Bracho MA, Arbona C, Larrea L, Díaz W, Sanjuán R, Cuevas JM. Exploring the Diversity of the Human Blood Virome. Viruses 2021; 13:v13112322. [PMID: 34835128 PMCID: PMC8621239 DOI: 10.3390/v13112322] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/22/2021] [Revised: 11/15/2021] [Accepted: 11/18/2021] [Indexed: 01/01/2023] Open
Abstract
Metagenomics is greatly improving our ability to discover new viruses, as well as their possible associations with disease. However, metagenomics has also changed our understanding of viruses in general. The vast expansion of currently known viral diversity has revealed a large fraction of non-pathogenic viruses, and offers a new perspective in which viruses function as important components of many ecosystems. In this vein, studies of the human blood virome are often motivated by the search for new viral diseases, especially those associated with blood transfusions. However, these studies have revealed the common presence of apparently non-pathogenic viruses in blood, particularly human anelloviruses and, to a lower extent, human pegiviruses (HPgV). To shed light on the diversity of the human blood virome, we subjected pooled plasma samples from 587 healthy donors in Spain to a viral enrichment protocol, followed by massive parallel sequencing. This showed that anelloviruses were clearly the major component of the blood virome and showed remarkable diversity. In total, we assembled 332 complete or near-complete anellovirus genomes, 50 of which could be considered new species. HPgV was much less frequent, but we, nevertheless, recovered 17 different isolates that we subsequently used for characterizing the diversity of this virus. In-depth investigation of the human blood virome should help to elucidate the ecology of these viruses, and to unveil potentially associated diseases.
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Affiliation(s)
- María Cebriá-Mendoza
- Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 València, Spain; (M.C.-M.); (W.D.); (R.S.)
| | - María A. Bracho
- Joint Research Unit “Infection and Public Health”, FISABIO-Universitat de València I2SysBio, 46020 València, Spain;
- CIBER in Epidemiology and Public Health (CIBERESP), 46020 València, Spain
| | - Cristina Arbona
- Centro de Transfusión de la Comunidad Valenciana, 46020 València, Spain; (C.A.); (L.L.)
| | - Luís Larrea
- Centro de Transfusión de la Comunidad Valenciana, 46020 València, Spain; (C.A.); (L.L.)
| | - Wladimiro Díaz
- Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 València, Spain; (M.C.-M.); (W.D.); (R.S.)
- Department of Informatics, Universitat de València, 46020 València, Spain
| | - Rafael Sanjuán
- Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 València, Spain; (M.C.-M.); (W.D.); (R.S.)
- Department of Genetics, Universitat de València, 46020 València, Spain
| | - José M. Cuevas
- Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, 46980 València, Spain; (M.C.-M.); (W.D.); (R.S.)
- Department of Genetics, Universitat de València, 46020 València, Spain
- Correspondence:
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Zimmerman J, Blackard JT. Human pegivirus type 1 infection in Asia-A review of the literature. Rev Med Virol 2021; 32:e2257. [PMID: 34038600 DOI: 10.1002/rmv.2257] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/10/2021] [Revised: 05/13/2021] [Accepted: 05/17/2021] [Indexed: 12/16/2022]
Abstract
The human pegivirus type 1 (HPgV-1)-as known as hepatitis G virus and GB virus C-is a common single-stranded RNA flavivirus. Because few studies have demonstrated an association between HPgV-1 infection and disease, screening for HPgV-1 is not performed routinely. Nonetheless, a beneficial impact of HPgV-1 infection on HIV disease progression has been reported in multiple studies. Given the burden of HIV in Asia and the complex interactions between viral co-infections and the host, we provide a comprehensive overview of the existing data from Asia on HPgV-1 infection, including the prevalence and circulating genotypes in all Asian countries with data reported. This review highlights the research conducted thus far and emphasizes the need for additional studies on HPgV-1 across the Asian continent.
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Affiliation(s)
- Joseph Zimmerman
- Division of Digestive Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
| | - Jason T Blackard
- Division of Digestive Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
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Human pegivirus-1 (HPgV-1, GBV-C) RNA prevalence and genotype diversity among volunteer blood donors from an intra-hospital hemotherapy service in Southern Brazil. Transfus Apher Sci 2019; 58:174-178. [DOI: 10.1016/j.transci.2019.01.002] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/22/2018] [Accepted: 01/22/2019] [Indexed: 01/06/2023]
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Assih M, Ouattara AK, Diarra B, Yonli AT, Compaore TR, Obiri-Yeboah D, Djigma FW, Karou S, Simpore J. Genetic diversity of hepatitis viruses in West-African countries from 1996 to 2018. World J Hepatol 2018; 10:807-821. [PMID: 30533182 PMCID: PMC6280160 DOI: 10.4254/wjh.v10.i11.807] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/02/2018] [Revised: 09/10/2018] [Accepted: 10/23/2018] [Indexed: 02/06/2023] Open
Abstract
The severity of hepatic pathology and the response to treatment depend on the hepatitis virus genotype in the infected host. The objective of this review was to determine the distribution of hepatitis virus genotypes in West African countries. A systematic review of the literature in PubMed, Google Scholar and Science Direct was performed to identify 52 relevant articles reporting hepatitis A, B, C, D, E and G viruses genotypes. Hepatitis B virus (HBV) genotype E with a prevalence of 90.6% (95%CI: 0.891-0.920) found in this review, is characterized by low genetic diversity. Hepatitis C virus (HCV) genotypes 1 and 2 represented 96.4% of HCV infections in West African countries, while hepatitis delta virus, hepatitis A virus, hepatitis G virus genotypes 1 and HEV genotype 3 were reported in some studies in Ghana and Nigeria. HBV genotype E is characterized by high prevalence, low genetic diversity and wide geographical distribution. Further studies on the clinical implications of HBV genotype E and HCV genotypes 1 and 2 are needed for the development of an effective treatment against this viral hepatitis in West African countries. Surveillance of the distribution of different genotypes is also needed to reduce recombination rates and prevent the emergence of more virulent viral strains.
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Affiliation(s)
- Maléki Assih
- Biochemistry-Microbiology, CERBA/LABIOGENE, Ouagadougou 02006, Burkina Faso
- Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Ouagadougou 00226, Burkina Faso
| | - Abdoul Karim Ouattara
- Biochemistry-Microbiology, CERBA/LABIOGENE, Ouagadougou 02006, Burkina Faso
- Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Ouagadougou 00226, Burkina Faso.
| | - Birama Diarra
- Biochemistry-Microbiology, CERBA/LABIOGENE, Ouagadougou 02006, Burkina Faso
- Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Ouagadougou 00226, Burkina Faso
| | - Albert Theophane Yonli
- Biochemistry-Microbiology, CERBA/LABIOGENE, Ouagadougou 02006, Burkina Faso
- Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Ouagadougou 00226, Burkina Faso
| | - Tegwindé Rebeca Compaore
- Biochemistry-Microbiology, CERBA/LABIOGENE, Ouagadougou 02006, Burkina Faso
- Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Ouagadougou 00226, Burkina Faso
| | - Dorcas Obiri-Yeboah
- Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Cape Coast 00233, Ghana
| | - Florencia Wendkuuni Djigma
- Biochemistry-Microbiology, CERBA/LABIOGENE, Ouagadougou 02006, Burkina Faso
- Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Ouagadougou 00226, Burkina Faso
| | - Simplice Karou
- Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA-UL), Universite de Lome, Lome 00229, Togo
| | - Jacques Simpore
- Biochemistry-Microbiology, CERBA/LABIOGENE, Ouagadougou 02006, Burkina Faso
- Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof Joseph KI-ZERBO, Ouagadougou 00226, Burkina Faso
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Jordier F, Deligny ML, Barré R, Robert C, Galicher V, Uch R, Fournier PE, Raoult D, Biagini P. Human pegivirus isolates characterized by deep sequencing from hepatitis C virus-RNA and human immunodeficiency virus-RNA-positive blood donations, France. J Med Virol 2018; 91:38-44. [PMID: 30133782 DOI: 10.1002/jmv.25290] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/28/2018] [Accepted: 08/14/2018] [Indexed: 01/18/2023]
Abstract
Human pegivirus (HPgV, formerly GBV-C) is a member of the genus Pegivirus, family Flaviviridae. Despite its identification more than 20 years ago, both natural history and distribution of this viral group in human hosts remain under exploration. Analysis of HPgV genomes characterized up to now points out the scarcity of French pegivirus sequences in databases. To bring new data regarding HPgV genomic diversity, we investigated 16 French isolates obtained from hepatitis C virus-RNA and human immunodeficiency virus-RNA-positive blood donations following deep sequencing and coupled molecular protocols. Initial phylogenetic analysis of 5'-untranslated region (5'-UTR)/E2 partial sequences permitted to assign HPgV isolates to genotypes 2 (n = 15) and 1 (n = 1), with up to 16% genetic diversity observed for both regions considered. Seven nearly full-length representative genomes were characterized subsequently, with complete polyprotein coding sequences exhibiting up to 13% genetic diversity; closest nucleotide (nt) divergence with available HPgV sequences was in the range 7% to 11%. A 36 nts deletion located on the NS4B coding region (N-terminal part, 12 amino acids) of the genotype 1 HPgV genome characterized was identified, along with single nucleotide deletions in two genotype 2, 5'-UTR sequences.
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Affiliation(s)
- François Jordier
- Biologie des Groupes Sanguins, Etablissement Français du Sang Provence Alpes Côte d'Azur Corse, Aix Marseille University, CNRS, EFS, ADES, Marseille, France
| | - Marie-Laurence Deligny
- Biologie des Groupes Sanguins, Etablissement Français du Sang Provence Alpes Côte d'Azur Corse, Aix Marseille University, CNRS, EFS, ADES, Marseille, France
| | - Romain Barré
- Biologie des Groupes Sanguins, Etablissement Français du Sang Provence Alpes Côte d'Azur Corse, Aix Marseille University, CNRS, EFS, ADES, Marseille, France
| | - Catherine Robert
- UMR MEPHI, IRD, Aix Marseille University, AP-HM, IHU Méditerranée-Infection, Marseille, France
| | - Vital Galicher
- Biologie des Groupes Sanguins, Etablissement Français du Sang Provence Alpes Côte d'Azur Corse, Aix Marseille University, CNRS, EFS, ADES, Marseille, France
| | - Rathviro Uch
- Biologie des Groupes Sanguins, Etablissement Français du Sang Provence Alpes Côte d'Azur Corse, Aix Marseille University, CNRS, EFS, ADES, Marseille, France
| | - Pierre-Edouard Fournier
- UMR VITROME, IRD, Aix Marseille University, SSA, AP-HM, IHU Méditerranée-Infection, Marseille, France
| | - Didier Raoult
- UMR MEPHI, IRD, Aix Marseille University, AP-HM, IHU Méditerranée-Infection, Marseille, France
| | - Philippe Biagini
- Biologie des Groupes Sanguins, Etablissement Français du Sang Provence Alpes Côte d'Azur Corse, Aix Marseille University, CNRS, EFS, ADES, Marseille, France
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9
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Singh S, Blackard JT. Human pegivirus (HPgV) infection in sub-Saharan Africa-A call for a renewed research agenda. Rev Med Virol 2017; 27. [PMID: 29148108 DOI: 10.1002/rmv.1951] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/12/2017] [Revised: 08/26/2017] [Accepted: 08/28/2017] [Indexed: 12/14/2022]
Abstract
The human pegivirus (HPgV)-formerly GB virus C-has a beneficial impact on HIV disease progression that has been described in multiple studies. Given the high prevalence of HIV in sub-Saharan Africa and the continuing need to suppress HIV replication, this review provides a comprehensive overview of the existing data on HPgV infection in sub-Saharan Africa, with a particular focus on studies of prevalence and the circulating HPgV genotypes. This review also highlights the need for additional studies of HPgV conducted on the African continent and proposes a research agenda for evaluation of HPgV.
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Affiliation(s)
- Shivank Singh
- Division of Digestive Diseases, University of Cincinnati College of Medicine, Cincinnati, OH, USA
| | - Jason T Blackard
- Division of Digestive Diseases, University of Cincinnati College of Medicine, Cincinnati, OH, USA
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10
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Horemheb-Rubio G, Ramos-Cervantes P, Arroyo-Figueroa H, Ávila-Ríos S, García-Morales C, Reyes-Terán G, Escobedo G, Estrada G, García-Iglesias T, Muñoz-Saucedo N, Kershenobich D, Ostrosky-Wegman P, Ruiz-Palacios GM. High HPgV replication is associated with improved surrogate markers of HIV progression. PLoS One 2017; 12:e0184494. [PMID: 28910347 PMCID: PMC5598987 DOI: 10.1371/journal.pone.0184494] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/24/2017] [Accepted: 08/24/2017] [Indexed: 02/06/2023] Open
Abstract
Background Human Pegivirus (HPgV) may have a beneficial effect on HIV disease progression in co-infected patients; however, the virologic characteristics of this infection are not well defined. In this study, we determined HPgV viremia prevalence in Mexico and provide new insights to understand HPgV infection and HPgV/HIV co-infection. Methods We analyzed and quantified 7,890 serum samples for HPgV viremia by One-Step RT-Real-Time PCR, 6,484 from healthy blood donors and 1,406 from HIV-infected patients. Data on HIV progression were obtained from patients’ records. HPgV genotyping was performed in 445 samples by nested PCR of the 5’URT region. Finite Mixture Models were used to identify clustering patterns of HPgV viremia in blood donors and co-infected antiretroviral (ART)-naïve patients. Results HPgV was detected in 2.98% of blood donors and 33% of HIV patients, with a wide range of viral loads. The most prevalent genotypes were 3 (58.6%)and 2 (33.7%). HPgV viral loads from healthy blood donors and HPgV/HIV+ ART-naïve co-infected patients were clustered into two component distributions, low and high, with a cut-off point of 5.07log10 and 5.06log10, respectively. High HPgV viremia was associated with improved surrogate markers of HIV infection, independent of the estimated duration of HIV infection or HIV treatment. Conclusions HPgV prevalence in Mexico was similar to that reported for other countries. The prevalent genotypes could be related to Mexico’s geographic location and ethnicity, since genotype 2 is frequent in the United States and Europe and genotype 3 in Asia and Amerindian populations. HPgV viral load demonstrated two patterns of replication, low and high. The more pronounced beneficial response observed in co-infected patients with high HPgV viremia may explain discrepancies found between other studies. Mechanisms explaining high and low HPgV replication should be explored to determine whether the persistently elevated replication depends on host or viral factors.
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Affiliation(s)
- Gibran Horemheb-Rubio
- Department of Infectious Diseases, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
| | - Pilar Ramos-Cervantes
- Department of Infectious Diseases, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
| | - Hugo Arroyo-Figueroa
- Department of Infectious Diseases, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
| | - Santiago Ávila-Ríos
- Infectious Diseases Research Center, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico
| | - Claudia García-Morales
- Infectious Diseases Research Center, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico
| | - Gustavo Reyes-Terán
- Infectious Diseases Research Center, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico
| | - Galileo Escobedo
- Liver Pancreas and Intestinal Motility Laboratory, Hospital General de México, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico
| | - Gloria Estrada
- Blood Bank, Hospital General de México, Mexico City, Mexico
| | - Trinidad García-Iglesias
- Immnuology Laboratory, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico
| | - Nayeli Muñoz-Saucedo
- Immnuology Laboratory, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico
| | - David Kershenobich
- Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
| | | | - Guillermo M. Ruiz-Palacios
- Department of Infectious Diseases, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico
- * E-mail:
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Wu H, Padhi A, Xu J, Gong X, Tien P. Evidence for Within-Host Genetic Recombination among the Human Pegiviral Strains in HIV Infected Subjects. PLoS One 2016; 11:e0161880. [PMID: 27560699 PMCID: PMC4999292 DOI: 10.1371/journal.pone.0161880] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/24/2016] [Accepted: 08/12/2016] [Indexed: 12/19/2022] Open
Abstract
The non-pathogenic Human Pegivirus (HPgV, formerly GBV-C/HGV), the most prevalent RNA virus worldwide, is known to be associated with reduced morbidity and mortality in HIV-infected individuals. Although previous studies documented its ubiquity and important role in HIV-infected individuals, little is known about the underlying genetic mechanisms that maintain high genetic diversity of HPgV within the HIV-infected individuals. To assess the within-host genetic diversity of HPgV and forces that maintain such diversity within the co-infected hosts, we performed phylogenetic analyses taking into account 229 HPgV partial E1-E2 clonal sequences representing 15 male and 8 female co-infected HIV patients from Hubei province of central China. Our results revealed the presence of eleven strongly supported clades. While nine clades belonged to genotype 3, two clades belonged to genotype 2. Additionally, four clades that belonged to genotype 3 exhibited inter-clade recombination events. The presence of clonal sequences representing multiple clades within the HIV-infected individual provided the evidence of co-circulation of HPgV strains across the region. Of the 23 patients, six patients (i.e., five males and one female) were detected to have HPgV recombinant sequences. Our results also revealed that while male patients shared the viral strains with other patients, viral strains from the female patients had restricted dispersal. Taken together, the present study revealed that multiple infections with divergent HPgV viral strains may have caused within-host genetic recombination, predominantly in male patients, and therefore, could be the major driver in shaping genetic diversity of HPgV.
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Affiliation(s)
- Haoming Wu
- College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China
| | - Abinash Padhi
- Department of Animal and Avian Sciences, University of Maryland, College Park, 20742, MD, United States of America
| | - Junqiang Xu
- Hubei Provincial Centers for Disease Control and Prevention, Wuhan 430072, Hubei, China
| | - Xiaoyan Gong
- College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, China
- * E-mail: (PT); (XG)
| | - Po Tien
- College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China
- Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
- * E-mail: (PT); (XG)
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