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1
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Su Q, Pan H, Hong P, You Y, Wu Y, Zou J, Sun J, Rao G, Liao J, Tang Z, Hu L. Protective effect of curcumin against endoplasmic reticulum stress and lipid metabolism disorders in AFB1-intoxicated duck liver. Mycotoxin Res 2025; 41:359-372. [PMID: 40085329 DOI: 10.1007/s12550-025-00586-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/03/2024] [Revised: 02/27/2025] [Accepted: 03/05/2025] [Indexed: 03/16/2025]
Abstract
Aflatoxin B1 (AFB1) is a stable and highly toxic toxin that causes multi-organ toxicity with sustained ingestion, most typically in the duck liver. Previous research has shown that AFB1 can bring about endoplasmic reticulum stress (ERS) in animals, and ERS is strongly associated with lipid metabolism. However, the relationship between AFB1-induced duck liver toxicity and ERS and lipid metabolism is currently unclear. Great attention has been paid to the prevention and treatment of AFB1 because of its great harm. Curcumin, a natural polyphenol, is notable for its powerful anti-inflammatory and antioxidant properties. Studies have shown curcumin to be protective against afb1-induced avian multi-organ toxicity. However, the effects of curcumin on the liver of ducks exposed to AFB1 are largely unknown. In the present study, we aimed to investigate whether AFB1 exposure induces ERS and lipid metabolism disorders in duck liver, while exploring the positive role of curcumin in it. One-day-old ducks (n = 80) were randomly divided in four groups: control group, AFB1 group (0.1 mg / kg.bw AFB1), Cur group (400 mg/kg curcumin), and AFB1 + Cur group (0.1 mg/kg.bw AFB1 + 400 mg/kg curcumin), and blood and liver were collected for the study after 21 days of continuous administration. Our research has found that AFB1 exposure significantly increases the levels of liver function indicators ALP, AST, and ALT in ducks' serum (P < 0.05). Duck liver undergoes fatty degeneration under the influence of AFB1. Under the effect of curcumin, AFB1-induced structural damage in duck liver was somewhat controlled. Further experimental results showed that AFB1 treatment significantly increased the expression of glucose-regulated protein 78 (P < 0.001), and activated the endoplasmic reticulum stress pathway. Meanwhile, AFB1 inhibited the LKB1-AMPK signaling pathway and disrupted lipid metabolic homeostasis. And curcumin treatment effectively reversed these changes. Overall, our results suggest that curcumin attenuates AFB1-induced hepatotoxicity in ducks by inhibiting ERS and lipid metabolism disorders.
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Affiliation(s)
- Qian Su
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Hang Pan
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Panjing Hong
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Yanli You
- College of Life Science, Yantai University, Yantai City, 264005, Shandong Province, China
| | - Yuhan Wu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Junbo Zou
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Jingping Sun
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Gan Rao
- Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou, China
| | - Jianzhao Liao
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Zhaoxin Tang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
| | - Lianmei Hu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
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2
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Badawy MEI, Taha MAI, Abdel-Razik RK, Abo-El-Saad MM. Preparation, characterization, and pesticide adsorption capacity of chitosan-magnetic graphene oxide nanoparticles with toxicological studies. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2025; 32:5159-5185. [PMID: 39907958 DOI: 10.1007/s11356-025-35975-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/10/2024] [Accepted: 01/16/2025] [Indexed: 02/06/2025]
Abstract
This study investigated magnetic graphene oxide nanoparticles (MGO-NPs) and functionalized with chitosan (CS-MGO-NPs) for removing florasulam, metalaxyl, and thiamethoxam pesticides from water. A comprehensive characterization employing Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), zeta potential measurements, XRD analysis, and surface area/porosity determinations confirmed the successful synthesis of the composites with the desired properties. Factorial experimental design was applied to identify the most significant factors of pesticide concentration, adsorbent amount, temperature, pH, agitation time, and ionic strength on the efficiency of removal of tested pesticides from water samples. CS-MGO-NPs exhibited superior removal efficiencies for all three pesticides compared to MGO-NPs. They achieved high removal rates for florasulam (average 92.94%) and metalaxyl (average 88.95%), while demonstrated moderate effectiveness against thiamethoxam (average 64.04%). Different kinetic and isotherm models described how well the nanoparticles adsorbed each pesticide. According to these models, the pseudo-first-order kinetic model interpreted well the adsorption of florasulam, and thiamethoxam onto CS-GO-NPs. While the pseudo-second-order kinetic model interpreted well the adsorption of metalaxyl. The Freundlich isotherm model gave the best fit with florasulam onto CS-GO-NPs. While the Langmuir isotherm model gave the best fit with metalaxyl and thiamethoxam. Finally, the toxicological studies of CS-MGO-NPs in rats were performed, and it was found negative effects at high doses, suggesting caution is needed for practical applications. Overall, this study shows promise for CS-MGO-NPs in water purification, but safety needs further investigation.
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Affiliation(s)
- Mohamed E I Badawy
- Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, 21545-El-Shatby, Alexandria, Egypt.
| | - Mostafa A I Taha
- Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, 21545-El-Shatby, Alexandria, Egypt
| | - Reda K Abdel-Razik
- Mammalian Toxicology Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 21616-El-Sabahia, Alexandria, Egypt
| | - Mahmoud M Abo-El-Saad
- Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, 21545-El-Shatby, Alexandria, Egypt
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3
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Makhado BP, Oladipo AO, Gumbi NN, De Kock LA, Andraos C, Gulumian M, Nxumalo EN. Unravelling the toxicity of carbon nanomaterials - From cellular interactions to mechanistic understanding. Toxicol In Vitro 2024; 100:105898. [PMID: 39029601 DOI: 10.1016/j.tiv.2024.105898] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/05/2024] [Revised: 07/03/2024] [Accepted: 07/13/2024] [Indexed: 07/21/2024]
Abstract
The application of carbon nanomaterials in diverse fields has substantially increased their demand for commercial usage. Within the earliest decade, the development of functional materials has further increased the significance of this element. Despite the advancements recorded, the potential harmful impacts of embracing carbon nanomaterials for biological applications must be balanced against their advantages. Interestingly, many studies have neglected the intriguing and dynamic cellular interaction of carbon nanomaterials and the mechanistic understanding of their property-driven behaviour, even though common toxicity profiles have been reported. Reiterating the toxicity issue, several researchers conclude that these materials have minimal toxicity and may be safe for contact with biological systems at certain dosages. Here, we aim to provide a report on the significance of some of the properties that influence their toxicity. After that, a description of the implication of nanotoxicology in humans and living systems, revealing piece by piece their exposure routes and possible risks, will be provided. Then, an extensive discussion of the mechanistic puzzle modulating the interface between various human cellular systems and carbon nanomaterials such as carbon nanotubes, carbon dots, graphene, fullerenes, and nanodiamonds will follow. Finally, this review also sheds light on the organization that handles the risk associated with nanomaterials.
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Affiliation(s)
- Bveledzani P Makhado
- Institute for Nanotechnology and Water Sustainability, College of Science, Engineering, and Technology, University of South Africa, Roodepoort 1710, South Africa
| | - Adewale O Oladipo
- Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Roodepoort 1710, South Africa
| | - Nozipho N Gumbi
- Institute for Nanotechnology and Water Sustainability, College of Science, Engineering, and Technology, University of South Africa, Roodepoort 1710, South Africa
| | - Lueta A De Kock
- Institute for Nanotechnology and Water Sustainability, College of Science, Engineering, and Technology, University of South Africa, Roodepoort 1710, South Africa
| | - Charlene Andraos
- Water Research Group, Unit for Environmental Sciences and Management, North-West University Potchefstroom, South Africa; National Institute for Occupational Health (NIOH), National Health Laboratory Service (NHLS), Johannesburg, South Africa; School of Public Health, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
| | - Mary Gulumian
- Water Research Group, Unit for Environmental Sciences and Management, North-West University Potchefstroom, South Africa
| | - Edward N Nxumalo
- Institute for Nanotechnology and Water Sustainability, College of Science, Engineering, and Technology, University of South Africa, Roodepoort 1710, South Africa.
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4
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Das SK, Sen K, Ghosh B, Ghosh N, Sinha K, Sil PC. Molecular mechanism of nanomaterials induced liver injury: A review. World J Hepatol 2024; 16:566-600. [PMID: 38689743 PMCID: PMC11056894 DOI: 10.4254/wjh.v16.i4.566] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/28/2023] [Revised: 02/05/2024] [Accepted: 03/19/2024] [Indexed: 04/24/2024] Open
Abstract
The unique physicochemical properties inherent to nanoscale materials have unveiled numerous potential applications, spanning beyond the pharmaceutical and medical sectors into various consumer industries like food and cosmetics. Consequently, humans encounter nanomaterials through diverse exposure routes, giving rise to potential health considerations. Noteworthy among these materials are silica and specific metallic nanoparticles, extensively utilized in consumer products, which have garnered substantial attention due to their propensity to accumulate and induce adverse effects in the liver. This review paper aims to provide an exhaustive examination of the molecular mechanisms underpinning nanomaterial-induced hepatotoxicity, drawing insights from both in vitro and in vivo studies. Primarily, the most frequently observed manifestations of toxicity following the exposure of cells or animal models to various nanomaterials involve the initiation of oxidative stress and inflammation. Additionally, we delve into the existing in vitro models employed for evaluating the hepatotoxic effects of nanomaterials, emphasizing the persistent endeavors to advance and bolster the reliability of these models for nanotoxicology research.
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Affiliation(s)
- Sanjib Kumar Das
- Department of Zoology, Jhargram Raj College, Jhargram 721507, India
| | - Koushik Sen
- Department of Zoology, Jhargram Raj College, Jhargram 721507, India
| | - Biswatosh Ghosh
- Department of Zoology, Bidhannagar College, Kolkata 700064, India
| | - Nabanita Ghosh
- Department of Zoology, Maulana Azad College, Kolkata 700013, India
| | - Krishnendu Sinha
- Department of Zoology, Jhargram Raj College, Jhargram 721507, India.
| | - Parames C Sil
- Department of Molecular Medicine, Bose Institute, Calcutta 700054, India
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5
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Lin H, Buerki-Thurnherr T, Kaur J, Wick P, Pelin M, Tubaro A, Carniel FC, Tretiach M, Flahaut E, Iglesias D, Vázquez E, Cellot G, Ballerini L, Castagnola V, Benfenati F, Armirotti A, Sallustrau A, Taran F, Keck M, Bussy C, Vranic S, Kostarelos K, Connolly M, Navas JM, Mouchet F, Gauthier L, Baker J, Suarez-Merino B, Kanerva T, Prato M, Fadeel B, Bianco A. Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective. ACS NANO 2024; 18:6038-6094. [PMID: 38350010 PMCID: PMC10906101 DOI: 10.1021/acsnano.3c09699] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/06/2023] [Revised: 01/18/2024] [Accepted: 01/22/2024] [Indexed: 02/15/2024]
Abstract
Two-dimensional (2D) materials have attracted tremendous interest ever since the isolation of atomically thin sheets of graphene in 2004 due to the specific and versatile properties of these materials. However, the increasing production and use of 2D materials necessitate a thorough evaluation of the potential impact on human health and the environment. Furthermore, harmonized test protocols are needed with which to assess the safety of 2D materials. The Graphene Flagship project (2013-2023), funded by the European Commission, addressed the identification of the possible hazard of graphene-based materials as well as emerging 2D materials including transition metal dichalcogenides, hexagonal boron nitride, and others. Additionally, so-called green chemistry approaches were explored to achieve the goal of a safe and sustainable production and use of this fascinating family of nanomaterials. The present review provides a compact survey of the findings and the lessons learned in the Graphene Flagship.
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Affiliation(s)
- Hazel Lin
- CNRS,
UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, ISIS, University of Strasbourg, 67000 Strasbourg, France
| | - Tina Buerki-Thurnherr
- Empa,
Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Particles-Biology Interactions, 9014 St. Gallen, Switzerland
| | - Jasreen Kaur
- Nanosafety
& Nanomedicine Laboratory, Institute
of Environmental Medicine, Karolinska Institutet, 177 77 Stockholm, Sweden
| | - Peter Wick
- Empa,
Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Particles-Biology Interactions, 9014 St. Gallen, Switzerland
| | - Marco Pelin
- Department
of Life Sciences, University of Trieste, 34127 Trieste, Italy
| | - Aurelia Tubaro
- Department
of Life Sciences, University of Trieste, 34127 Trieste, Italy
| | | | - Mauro Tretiach
- Department
of Life Sciences, University of Trieste, 34127 Trieste, Italy
| | - Emmanuel Flahaut
- CIRIMAT,
Université de Toulouse, CNRS, INPT,
UPS, 31062 Toulouse CEDEX 9, France
| | - Daniel Iglesias
- Facultad
de Ciencias y Tecnologías Químicas, Universidad de Castilla-La Mancha (UCLM), 13071 Ciudad Real, Spain
- Instituto
Regional de Investigación Científica Aplicada (IRICA), Universidad de Castilla-La Mancha (UCLM), 13071 Ciudad Real, Spain
| | - Ester Vázquez
- Facultad
de Ciencias y Tecnologías Químicas, Universidad de Castilla-La Mancha (UCLM), 13071 Ciudad Real, Spain
- Instituto
Regional de Investigación Científica Aplicada (IRICA), Universidad de Castilla-La Mancha (UCLM), 13071 Ciudad Real, Spain
| | - Giada Cellot
- International
School for Advanced Studies (SISSA), 34136 Trieste, Italy
| | - Laura Ballerini
- International
School for Advanced Studies (SISSA), 34136 Trieste, Italy
| | - Valentina Castagnola
- Center
for
Synaptic Neuroscience and Technology, Istituto
Italiano di Tecnologia, 16132 Genova, Italy
- IRCCS
Ospedale Policlinico San Martino, 16132 Genova, Italy
| | - Fabio Benfenati
- Center
for
Synaptic Neuroscience and Technology, Istituto
Italiano di Tecnologia, 16132 Genova, Italy
- IRCCS
Ospedale Policlinico San Martino, 16132 Genova, Italy
| | - Andrea Armirotti
- Analytical
Chemistry Facility, Istituto Italiano di
Tecnologia, 16163 Genoa, Italy
| | - Antoine Sallustrau
- Département
Médicaments et Technologies pour la Santé (DMTS), Université Paris-Saclay, CEA, INRAE, SIMoS, Gif-sur-Yvette 91191, France
| | - Frédéric Taran
- Département
Médicaments et Technologies pour la Santé (DMTS), Université Paris-Saclay, CEA, INRAE, SIMoS, Gif-sur-Yvette 91191, France
| | - Mathilde Keck
- Département
Médicaments et Technologies pour la Santé (DMTS), Université Paris-Saclay, CEA, INRAE, SIMoS, Gif-sur-Yvette 91191, France
| | - Cyrill Bussy
- Nanomedicine
Lab, Faculty of Biology, Medicine and Health, University of Manchester,
Manchester Academic Health Science Centre, National Graphene Institute, Manchester M13 9PT, United
Kingdom
| | - Sandra Vranic
- Nanomedicine
Lab, Faculty of Biology, Medicine and Health, University of Manchester,
Manchester Academic Health Science Centre, National Graphene Institute, Manchester M13 9PT, United
Kingdom
| | - Kostas Kostarelos
- Nanomedicine
Lab, Faculty of Biology, Medicine and Health, University of Manchester,
Manchester Academic Health Science Centre, National Graphene Institute, Manchester M13 9PT, United
Kingdom
| | - Mona Connolly
- Instituto Nacional de Investigación y Tecnología
Agraria
y Alimentaria (INIA), CSIC, Carretera de la Coruña Km 7,5, E-28040 Madrid, Spain
| | - José Maria Navas
- Instituto Nacional de Investigación y Tecnología
Agraria
y Alimentaria (INIA), CSIC, Carretera de la Coruña Km 7,5, E-28040 Madrid, Spain
| | - Florence Mouchet
- Laboratoire
Ecologie Fonctionnelle et Environnement, Université de Toulouse, CNRS, INPT, UPS, 31000 Toulouse, France
| | - Laury Gauthier
- Laboratoire
Ecologie Fonctionnelle et Environnement, Université de Toulouse, CNRS, INPT, UPS, 31000 Toulouse, France
| | - James Baker
- TEMAS Solutions GmbH, 5212 Hausen, Switzerland
| | | | - Tomi Kanerva
- Finnish Institute of Occupational Health, 00250 Helsinki, Finland
| | - Maurizio Prato
- Center
for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), 20014 Donostia-San
Sebastián, Spain
- Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain
- Department
of Chemical and Pharmaceutical Sciences, University of Trieste, 34127 Trieste, Italy
| | - Bengt Fadeel
- Nanosafety
& Nanomedicine Laboratory, Institute
of Environmental Medicine, Karolinska Institutet, 177 77 Stockholm, Sweden
| | - Alberto Bianco
- CNRS,
UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, ISIS, University of Strasbourg, 67000 Strasbourg, France
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6
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Gendron D, Bubak G. Carbon Nanotubes and Graphene Materials as Xenobiotics in Living Systems: Is There a Consensus on Their Safety? J Xenobiot 2023; 13:740-760. [PMID: 38132708 PMCID: PMC10744618 DOI: 10.3390/jox13040047] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/16/2023] [Revised: 11/27/2023] [Accepted: 11/28/2023] [Indexed: 12/23/2023] Open
Abstract
Carbon nanotubes and graphene are two types of nanomaterials that have unique properties and potential applications in various fields, including biomedicine, energy storage, and gas sensing. However, there is still a debate about the safety of these materials, and there is yet to be a complete consensus on their potential risks to human health and the environment. While some studies have provided recommendations for occupational exposure limits, more research is needed to fully understand the potential risks of these materials to human health and the environment. In this review, we will try to summarize the advantages and disadvantages of using carbon nanotubes and graphene as well as composites containing them in the context of their biocompatibility and toxicity to living systems. In addition, we overview current policy guidelines and technical regulations regarding the safety of carbon-based nanomaterials.
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Affiliation(s)
- David Gendron
- Kemitek, Cégep de Thetford, 835 Rue Mooney, Thetford Mines, QC G6G 0A5, Canada
| | - Grzegorz Bubak
- Institute of Physical Chemistry, Polish Academy of Sciences, 01-224 Warsaw, Poland;
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7
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Yang S, Hu H, Kung H, Zou R, Dai Y, Hu Y, Wang T, Lv T, Yu J, Li F. Organoids: The current status and biomedical applications. MedComm (Beijing) 2023; 4:e274. [PMID: 37215622 PMCID: PMC10192887 DOI: 10.1002/mco2.274] [Citation(s) in RCA: 57] [Impact Index Per Article: 28.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/31/2022] [Revised: 04/22/2023] [Accepted: 04/27/2023] [Indexed: 05/24/2023] Open
Abstract
Organoids are three-dimensional (3D) miniaturized versions of organs or tissues that are derived from cells with stem potential and can self-organize and differentiate into 3D cell masses, recapitulating the morphology and functions of their in vivo counterparts. Organoid culture is an emerging 3D culture technology, and organoids derived from various organs and tissues, such as the brain, lung, heart, liver, and kidney, have been generated. Compared with traditional bidimensional culture, organoid culture systems have the unique advantage of conserving parental gene expression and mutation characteristics, as well as long-term maintenance of the function and biological characteristics of the parental cells in vitro. All these features of organoids open up new opportunities for drug discovery, large-scale drug screening, and precision medicine. Another major application of organoids is disease modeling, and especially various hereditary diseases that are difficult to model in vitro have been modeled with organoids by combining genome editing technologies. Herein, we introduce the development and current advances in the organoid technology field. We focus on the applications of organoids in basic biology and clinical research, and also highlight their limitations and future perspectives. We hope that this review can provide a valuable reference for the developments and applications of organoids.
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Affiliation(s)
- Siqi Yang
- Division of Biliary Tract SurgeryDepartment of General SurgeryWest China HospitalSichuan UniversityChengduSichuan ProvinceChina
| | - Haijie Hu
- Division of Biliary Tract SurgeryDepartment of General SurgeryWest China HospitalSichuan UniversityChengduSichuan ProvinceChina
| | - Hengchung Kung
- Krieger School of Arts and SciencesJohns Hopkins UniversityBaltimoreMarylandUSA
| | - Ruiqi Zou
- Division of Biliary Tract SurgeryDepartment of General SurgeryWest China HospitalSichuan UniversityChengduSichuan ProvinceChina
| | - Yushi Dai
- Division of Biliary Tract SurgeryDepartment of General SurgeryWest China HospitalSichuan UniversityChengduSichuan ProvinceChina
| | - Yafei Hu
- Division of Biliary Tract SurgeryDepartment of General SurgeryWest China HospitalSichuan UniversityChengduSichuan ProvinceChina
| | - Tiantian Wang
- Key Laboratory of Rehabilitation Medicine in Sichuan ProvinceWest China HospitalSichuan UniversityChengduSichuanChina
| | - Tianrun Lv
- Division of Biliary Tract SurgeryDepartment of General SurgeryWest China HospitalSichuan UniversityChengduSichuan ProvinceChina
| | - Jun Yu
- Departments of MedicineJohns Hopkins University School of MedicineBaltimoreMarylandUSA
- Departments of OncologyJohns Hopkins University School of MedicineBaltimoreMarylandUSA
| | - Fuyu Li
- Division of Biliary Tract SurgeryDepartment of General SurgeryWest China HospitalSichuan UniversityChengduSichuan ProvinceChina
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8
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Rhazouani A, Gamrani H, Ed-Day S, Lafhal K, Boulbaroud S, Gebrati L, Fdil N, AZIZ F. Sub-acute toxicity of graphene oxide (GO) nanoparticles in male mice after intraperitoneal injection: Behavioral study and histopathological evaluation. Food Chem Toxicol 2023; 171:113553. [DOI: 10.1016/j.fct.2022.113553] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/08/2022] [Revised: 12/01/2022] [Accepted: 12/05/2022] [Indexed: 12/14/2022]
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9
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Cheng TM, Chu HY, Huang HM, Li ZL, Chen CY, Shih YJ, Whang-Peng J, Cheng RH, Mo JK, Lin HY, Wang K. Toxicologic Concerns with Current Medical Nanoparticles. Int J Mol Sci 2022; 23:7597. [PMID: 35886945 PMCID: PMC9322368 DOI: 10.3390/ijms23147597] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/17/2022] [Revised: 06/28/2022] [Accepted: 07/05/2022] [Indexed: 02/05/2023] Open
Abstract
Nanotechnology is one of the scientific advances in technology. Nanoparticles (NPs) are small materials ranging from 1 to 100 nm. When the shape of the supplied nanoparticles changes, the physiological response of the cells can be very different. Several characteristics of NPs such as the composition, surface chemistry, surface charge, and shape are also important parameters affecting the toxicity of nanomaterials. This review covered specific topics that address the effects of NPs on nanomedicine. Furthermore, mechanisms of different types of nanomaterial-induced cytotoxicities were described. The distributions of different NPs in organs and their adverse effects were also emphasized. This review provides insight into the scientific community interested in nano(bio)technology, nanomedicine, and nanotoxicology. The content may also be of interest to a broad range of scientists.
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Affiliation(s)
- Tsai-Mu Cheng
- Graduate Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan; (T.-M.C.); (H.-Y.C.)
- Taipei Heart Institute, Taipei Medical University, Taipei 11031, Taiwan
| | - Hsiu-Yi Chu
- Graduate Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan; (T.-M.C.); (H.-Y.C.)
| | - Haw-Ming Huang
- School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan;
| | - Zi-Lin Li
- Graduate Institute of Nanomedicine and Medical Engineering, College of Medical Engineering, Taipei Medical University, Taipei 11031, Taiwan; (Z.-L.L.); (C.-Y.C.); (Y.-J.S.)
- Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan;
| | - Chiang-Ying Chen
- Graduate Institute of Nanomedicine and Medical Engineering, College of Medical Engineering, Taipei Medical University, Taipei 11031, Taiwan; (Z.-L.L.); (C.-Y.C.); (Y.-J.S.)
- Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan;
| | - Ya-Jung Shih
- Graduate Institute of Nanomedicine and Medical Engineering, College of Medical Engineering, Taipei Medical University, Taipei 11031, Taiwan; (Z.-L.L.); (C.-Y.C.); (Y.-J.S.)
- Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan;
| | | | - R. Holland Cheng
- Department of Molecular & Cellular Biology, University of California, Davis, CA 95616, USA;
| | - Ju-Ku Mo
- Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan;
| | - Hung-Yun Lin
- Cancer Center, Wan Fang Hospital, Taipei Medical University, Taipei 11031, Taiwan;
- Department of Molecular & Cellular Biology, University of California, Davis, CA 95616, USA;
- TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 11031, Taiwan
- Traditional Herbal Medicine Research Center of Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan
- Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Albany, NY 12208, USA
| | - Kuan Wang
- Graduate Institute of Nanomedicine and Medical Engineering, College of Medical Engineering, Taipei Medical University, Taipei 11031, Taiwan; (Z.-L.L.); (C.-Y.C.); (Y.-J.S.)
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Biological Responses in the Blood and Organs of Rats to Intraperitoneal Inoculation of Graphene and Graphene Oxide. MATERIALS 2022; 15:ma15082898. [PMID: 35454592 PMCID: PMC9029982 DOI: 10.3390/ma15082898] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/18/2022] [Revised: 04/10/2022] [Accepted: 04/13/2022] [Indexed: 02/05/2023]
Abstract
Background: The discrepancy among the in vivo results found in the literature regarding graphene’s side effects led us to conduct an in vivo study with graphene. Methods: In vivo tests involving intraperitoneal inoculation of graphene and graphene oxide nanosheets in rats were carried out to assess potential changes in the blood and organs after 15 and 30 days. Graphene and graphene oxide nanosheets at a concentration of 4 mg per kilogram were suspended in an aqueous solution of 0.9% NaCl at a 1:1 proportion (graphene or graphene oxide), i.e., 1 mg/mL. Results: Optical microscopy of liver, kidney, spleen, and lung tissues revealed no visible histological changes. However, particle traces were found in the peritoneal cavity. Thirty days after inoculation, blood samples were collected for hematological analysis. The blood analysis showed changes indicating a hepatic inflammatory process. Hematological changes after 30 days consisted of alterations to the red series, including microcytosis or higher mean hemoglobin concentrations. In addition, changes in prothrombin and thromboplastin caused longer coagulation times. Conclusion: This study contributes to further clarifying the possible toxicity of graphene and its potential biomedical applications.
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Safety assessment of graphene oxide and microcystin-LR complex: a toxicological scenario beyond physical mixture. Part Fibre Toxicol 2022; 19:26. [PMID: 35392949 PMCID: PMC8988332 DOI: 10.1186/s12989-022-00466-x] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/06/2021] [Accepted: 03/29/2022] [Indexed: 11/16/2022] Open
Abstract
Background Nanomaterials have been widely used in electrochemistry, sensors, medicine among others applications, causing its inevitable environmental exposure. A raising question is the “carrier” effect due to unique surface properties of nanomaterials, which may collectively impact the bioavailability, toxicokinetic, distribution and biological effects of classic toxicants. Noteworthy, this aspect of information remains largely unexplored. Methods Here, we deliberately selected two entities to mimic this scenario. One is graphene oxide (GO), which is made in ton quantity with huge surface-area that provides hydrophilicity and π–π interaction to certain chemicals of unique structures. The other is Microcystin-LR (MCLR), a representative double-bond rich liver-toxic endotoxin widely distributed in aquatic-system. Firstly, the adsorption of GO and MCLR after meeting under environmental conditions was explored, and then we focused on the toxicological effect and related mechanism of GO-MCLR complex on human skin cutin forming cells (HaCaT cells) and normal liver cells (L02 cells). Results Abiotically, our study demonstrated that GO could effectively adsorb MCLR through hydrogen bonding and π–π interaction, the oxidation degree of GO-MCLR decreased significantly and surface defect level raised. Compared to GO or MCLR, GO-MCLR was found to induce more remarkable apoptosis and ferroptosis in both HaCaT and L02 cells. The underlying mechanism was that GO-MCLR induced stronger intracellular reactive oxygen species (ROS) and mtROS generation, followed by Fe2+ accumulation, mitochondrial dysfunction and cytoskeletal damage. Conclusions These results suggest that the GO-MCLR complex formed by GO adsorption of MCLR may exhibit more toxic effects than the single material, which demonstrates the necessity for assessing nano-toxicant complexity. Our discovery may serve as a new toxicological paradigm in which nanomaterial mediated surface adsorption effects could impact the degree of cytotoxicity and toxicological mechanisms of classic toxins. Graphical Abstract ![]()
Supplementary Information The online version contains supplementary material available at 10.1186/s12989-022-00466-x.
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