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Copyright ©2014 Baishideng Publishing Group Inc.
World J Stem Cells. Jul 26, 2014; 6(3): 288-295
Published online Jul 26, 2014. doi: 10.4252/wjsc.v6.i3.288
Table 1 In-vitro osteogenesis potential between human bone marrow mesenchymal stem cells and adipose-derived stem cells
Ref.Osteogenic mediumCulturing conditionAnalytic methodsOutcome of osteogenic ability
Park et al[22]0.1 mmol/L nonessential ammino acids, 50 μg/mL ascorbic acid-2-phosphate, 100 nmol/L dexamethasone and 10 mmol/L β-glycerolphosphateMechanical stimulation by dynamic hydraulic compressionAP, Alizarin red stian, qPCR, immunofluorescenceBMSC > ASC under mechanical stimulation
Vishnubalaji et al[20]10 nmol/L calcitriol, 10 nmol/L β-glycerophosphate, 50 μg/mL L-ascorbic acid, 10 nmol/L dexamethasone2-D static cutureAP, Alizarin red, Von Kossa stain, Calcium concentration, qPCRBMSC > ASC
De Ugarte et al[16]50 μg/mL ascorbic acid-2-phosphate, 100 nmol/L dexamethasone and 10 mmol/L β-glycerolphosphate2-D static cultureAP activity, calcium assayBMSC = ASC
Im et al[17]100 nmol/L dexamethasone, 50 μmol/L ascorbate-2- phosphate, 10 mmol/L dexamethasone β-glycerophosphate2D static cultureAP activity, Von Kossa stainingBMSC > ASC
Shafiee et al[19]10 nmol/L dexamethason, 0.2 mmol/L ascorbic acid-2-phosphate, 10 mmol/L β-glycerophosphate2D cell cultureAP activity, Alizarin red staining, qPCRBMSC > ASC
Liu et al[18]0.1 μmol/L dexamethasone, 50 μmol/L ascorbic acid-2-phosphate and 10 mmol/L β-glycerophosphate2D cell cultureAlizarin red stain and microarrayBMSC > ASC
Zhang et al[21]10 mmol/L β-glycerophosphate, 10-8 mol/L dexamethasone, and 0.2 mmol/L ascorbic acid2D cell culture 3D cell cultureCalcium assay, qPCR, SEMBMSC > ASC
Table 2 Osteogenesis potential between bone marrow mesenchymal stem cells and adipose-derived stem cells in animal model
Ref.Animal modelScaffoldAnalytic methodsOutcome of osteogenic ability
Hayashi et al[27]Subcutaneous implantation in ratHydroxyapatiteMicro-CTNew bone volume BMSC (6.85 ± 1.89 mm3) > ASC (0.05 ± 0.05 mm3)
Niemeyer et al[32]3-cm tibial defect in sheepCollagen spongeRadiographic and histologic analysisBMSC > ASC BMSC = ASC + PRP No original data for bone volume
Kang et al[23]1.5-cm radial bone defect in dogTricalcium phosphateRadiographic, histological and histomorphometric analysisNew bone percentage: BMSC (33.56%) = ASC (33.9%)
Stockmann et al[33]1-cm calvarial bone defect in pigBovine collagen type IMicroradiography, histomorphometric evaluationBMSC = ASC No original data for bone volume
Wen et al[34]5-mm calvarial defect in SD ratCollagen gelX-ray and histologyBMSC = ASC No original data for bone volume
Zhang et al[21]Subcutaneous pocket of nude micePCL/TCPMicro-CTNew bone volume: BMSC (16.6 ± 3.0 mm3) > ASC (9.1 ± 1.1 mm3)