Conditions to improve expansion of human mesenchymal stem cells based on rat samples

Table 1 The number of rat mesenchymal stem cells cultured in Dulbecco's modified Eagle's medium low glucose containing different concentration of fetal calf serum

Time (wk)	Cell number per T25 culture plate
	5% FCS	10% FCS	15% FCS
2	6 × 105± 1%	6 × 105± 1%	6 × 105± 1%
3	8 × 105± 1%	1 × 106± 1%	2 × 106± 1%
4	1 × 106± 1%	3 × 106± 1%	4 × 106± 1%
5	1.5 × 106± 1%	6 × 106± 1%	8 × 106± 1%
6	1.8 × 106± 1%	10 × 106± 1%	12 × 106± 1%
7	1.7 × 106± 1%	13 × 106± 1%	15 × 106± 1%

FSC: Fetal calf serum.