Density gradient centrifugation vs adherence for rat intestinal mesenchymal stem cell isolation

Table 1 Comparison of surface marker expression in passage 4 cells between the two groups (%, mean ± SEM, n = 3)

Marker	Group A	Group B	P values
CD90+	70.63% ± 1.15%	93.70% ± 0.20%	< 0.001
CD73+	81.93% ± 0.06%	91.67% ± 0.21%	< 0.001
CD44+	60.50% ± 5.85%	90.7% ± 0.36%	< 0.01
CD34+	3.24% ± 0.28%	0.52% ± 0.35%	< 0.01
CD45+	3.54% ± 3.84%	4.59% ± 3.40%	> 0.05
CD11b/c+	1.02% ± 1.05%	1.44% ± 1.17%	> 0.05

Table 2 CCK-8 assay results of passage 4 cells from the two groups (OD450, mean ± SEM, n = 5)

Culture time	Group A	Group B	P values
Day 0	0.105 ± 0.006	0.102 ± 0.005	> 0.05
Day 1	0.152 ± 0.008	0.148 ± 0.007	> 0.05
Day 2	0.213 ± 0.012	0.221 ± 0.010	> 0.05
Day 3	0.345 ± 0.018	0.412 ± 0.015	< 0.05
Day 4	0.521 ± 0.025	0.678 ± 0.022	< 0.01
Day 5	0.734 ± 0.032	0.998 ± 0.028	< 0.01
Day 6	0.892 ± 0.035	1.356 ± 0.031	< 0.001