Proliferation and tenogenic differentiation of bone marrow mesenchymal stem cells in a porous collagen sponge scaffold

doi:10.4252/wjsc.v13.i1.115

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Jan 26, 2021; 13(1): 115-127
Published online Jan 26, 2021. doi: 10.4252/wjsc.v13.i1.115

Table 1 Primer sequences utilized for real-time polymerase chain reaction

Gene	5’-3’	Primer sequence	Product size (bp)	Reference sequence
Col1a1	Forward Reverse	GGAGAGAGTGCCAACTCCAG; GTGCTTTGGAAAATGGTGCT	182	NM_053304.1
Col3a1	Forward Reverse	TCCCAGAACATTACATACCACT; GCTATTTCCTTCAGCCTTGA	126	NM_032085.1
Tnc	Forward Reverse	AGATGCTACTCCAGACGGTTTC; CACGGCTTATTCCATAGAGTTCA	200	NM_053861.1
Scx	Forward Reverse	AACACGGCCTTCACTGCGCTG; CAGTAGCACGTTGCCCAGGTG	123	NM_001130508.1
Tnmd	Forward Reverse	GTGGTCCCACAAGTGAAGGT; GTCTTCCTCGCTTGCTTGTC	60	NM_022290.1
PPARγ	Forward Reverse	CAGGCTTGCTGAACGTGAAG; ACGTGCTCTGTGACAATCTGC	157	NM_001145366.1
Runx2	Forward Reverse	CAGACCAGCAGCACTCCATA; CAGCGTCAACACCATCATTC	178	NM_001278483.1
GAPDH	Forward Reverse	AAGTTCAACGGCACAGTCAAGG; CGCCAGTAGACTCCACGACATA	139	NM_017008.4

Citation: Zhang BY, Xu P, Luo Q, Song GB. Proliferation and tenogenic differentiation of bone marrow mesenchymal stem cells in a porous collagen sponge scaffold. World J Stem Cells 2021; 13(1): 115-127
URL: https://www.wjgnet.com/1948-0210/full/v13/i1/115.htm
DOI: https://dx.doi.org/10.4252/wjsc.v13.i1.115