Bone marrow mesenchymal stem cells induce M2 microglia polarization through PDGF-AA/MANF signaling

Table 1 Primers used for real-time quantitative reverse transcription polymerase chain reaction analysis

Gene	Forward primer (5'–3')	Reverse primer (5'–3')
MANF	TCCGCTACTGTAAGCAAGGT	CTTCACCTAGGATCTTGGTG
PDGF-AA	GCCATTCCCGCAGTTTG	GGCTGGCACTTGACGCT
XBP1	ATGTTTTTCAAATGTCCTTCCCCAG	TGACAGAGAAAGGGAGGCTGGTAAG
ATF6	TGCAGGTGTATTACGCTTCGC	GCAGGTGATCCCTTCGAAATG
iNOS	ATCCCGAAACGCTACACTT	CGGCTGGACTTCTCACTC
Arg1	CAGTGGCGTTGACCTTGT	TGGTTCTGTTCGGTTTGC
NF-YA	CTGAGACTCCACAGCCATCA	GGATCTTCCCTTCTGCCTCT
NF-YB	CTGGATGGGGCTGACTGTAT	AGGAGGTACGCCAGTCTGTG
NF-YC	ACCCTTGCATGGCACTAAAG	GCTGGATGGTGGTCGTAGAA
miR-30a*	CTTTCAGTCGGATGTTTGC	GTGCGTGTCGTGGAGTCG
GAPDH	GCAAGTTCAACGGCACAG	GCCAGTAGACTCCACGACAT
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT

MANF: Mesencephalic astrocyte–derived neurotrophic factor; PDGF-AA: Platelet-derived growth factor-AA; XBP1: X-box binding protein 1; ATF6: Activating transcription factor 6; iNOS: Inducible nitric oxide synthase; Arg-1: Arginase-1; NF-Y: Nuclear factor-Y; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.