Basic Study
Copyright ©The Author(s) 2019.
World J Stem Cells. Jun 26, 2019; 11(6): 347-374
Published online Jun 26, 2019. doi: 10.4252/wjsc.v11.i6.347
Table 1 Antibodies used for immunofluorescence and for flow cytometry analysis
AntigenCompanyDilutionIncubation time
Immunofluorescence staining
CD73Thermo FisherWeston, Florida, USA1:1001 h
CD90AbcamCambridge, UK1:1001 h
CD105DakoPalo Alto, California, USA1:101 h
CD341:10030 min
CD451:10030 min
CD561:8030 min
CD146Santa CruzDallas, Tx, USA1:251 h
PDGFRα1:251 h
PW1Atlas AntibodiesBromma, Sweden1:1001 h
Alexa Fluor1:70040 min
488 nm
Donkey anti-mouse
Alexa FluorThermo FisherWeston, Florida, USA1:70040 min
488 nm
Donkey anti-rabbit
Alexa Fluor1:70040 min
594 nm
Donkey anti-mouse
Alexa Fluor1:70040 min
594 nm
Donkey anti-rabbit
Flow Cytometry
CD73 (IgG) APCBD PharmingenSan Jose, California, USA1:2530 min
CD90 (IgG) FITC1:2530 min
CD105 (IgG) PE1:2530 min
CD34 (IgG) FITC1:2530 min
CD45 (IgG) APC1:2530 min
CD56 (IgG) PE1:2530 min
CD146 (IgG) PE1:2530 min
Isotype controls:1:2530 min
IgG APC
IgG PE
IgG FITC
Table 2 Summary of biological properties of MSCs isolated from different tissues
Summary
PhenotypeMarkerBM-MSCAT-MSCSM-MSCSK-MSC
P1P5P10P1P5P10P1P5P10P1P5P10
CD73++++++++++++++++++++++++++++++++++++
CD90++++++++++++++++++++++++++++++++++
CD105+++++++++++++++++++++++++++
CD146++++++++++++/-+++/-++++
PDGFRα+++++/-+++++/-+++++++
PW1-+/-+/-+++/-+/-++++/-+/-+++/-+/-
DifferentiationTissueBM-MSCAT-MSCSM-MSCSK-MSC
P1P5P10P1P5P10P1P5P10P1P5P10
Osteo-+++++++++++++++++++++++++
Adipo-+++++++++++++---++++/-
Chondro-++++++++++++++++++++++++
mRNA ecpressionGeneBM-MSCAT-MSCSM-MSCSK-MSC
P1P5P10P1P5P10P1P5P10P1P5P10
Sox2+++++++++++++++++
Oct4++++++++++++/-++++
p53+++++++++++++++++++++
c-myc++++++++++++++++++++++++++
Cytokines and chemokinesCytokineBM-MSCAT-MSCSM-MSCSK-MSC
P1P5P10P1P5P10P1P5P10P1P5P10
MCP-1++++++/-+++++++++
IL-8++++++++/-+++--+++++
VEGF+++++++-++/-+++/--+/--
IL-5+/-+/-+/-+/-+/-+/-+/-+/-+/----
IFN-γ+/-++/-+/-+/-++/-++/-+/-+/-+/-
IL-6+/-+++++++++++/-++-+/--
MIP-1β++++++-+/-++/-+/-+/-