Copyright ©The Author(s) 2020.
World J Stem Cells. Aug 26, 2020; 12(8): 814-840
Published online Aug 26, 2020. doi: 10.4252/wjsc.v12.i8.814
Table 2 Summary of exosome isolation methods
UltracentrifugationPhysical methodA golden standard; low cost; a wide range of volumesLow yield; low purity; time-consuming
Membrane filtrationPhysical method using filtersSimple; fast; high yield; keeps exosomes intactLow purity; deformation of exosomes
PrecipitationPhysical/chemical methodHigh yield; easy; high recoveriesLow purity; contaminants
Size exclusion chromatographyUse columns packed with pore beadsHigh yield; reduces exosome aggregation; keeps exosomes intactA small number of bands; time-consuming
Immunoaffinity capture technologyMagnetic beads bound to specific antibodiesHigh yield; high purity; specialtyTime-consuming; high cost