Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model

Figure 1 Culture system enabling seeding of corneal-stroma derived stem cells on amniotic membrane. A: Schematic showing dimensions and arrangement of polytetrafluoroethylene (PTFE) O-ring inside 30 mm tissue culture insert; B: The PTFE ring is placed on the epithelial side of hydrated amniotic membrane (AM); C: The AM is firmly wrapped over the O-ring edges leaving a taut membrane surface for cell seeding; D: The O-ring containing the AM is placed within the tissue culture insert; E: The tissue culture insert is placed within a 6-well plate and culture medium applied.