Next-generation sequencing traces human induced pluripotent stem cell lines clonally generated from heterogeneous cancer tissue

Figure 1 Phase contrast micrographs of colon cancer tissue-derived induced pluripotent stem cells. The human iPSC line CC1-1 was expanded with mitomycin C-treated mouse embryonic fibroblasts in gelatin-coated dishes (upper left panel: × 10, upper right panel: × 20) and cultured with feeder-free mTeSR1 medium in BD Matrigel^TM-coated dishes (lower left panel: × 10, lower right panel: × 20). iPSC: Induced pluripotent stem cell; MEF: Mouse embryonic fibroblast.

Figure 2 Pipeline of bioinformatics analysis following next-generation sequencing. The thirteen confirmed SNVs are shown in Tables 6-8. SNVs: Single-nucleotide variants.