Original Article
Copyright ©2011 Baishideng Publishing Group Co.
World J Stem Cells. Jul 26, 2011; 3(7): 63-69
Published online Jul 26, 2011. doi: 10.4252/wjsc.v3.i7.63
Figure 1
Figure 1 Flow cytometry of CD90+ CD73+ CD45- cells in peripheral blood cultures. CD90+, CD73+ CD45- cells in peripheral blood of control and cirrhosis cultures detected by flow cytometry. After 14 d cultured cells were stained with anti CD90, anti CD73 antibodies and analyzed on FACS Aria II. A: depicts increased number of CD90+, CD73+ cells in cultures of cirrhosis patients (7.09%) (P < 0.001) compared with controls (0.48%); B: Quantitative representation of the cells in cultures.bP < 0.001.
Figure 2
Figure 2 Morphological features of cells in cultures. Morphological features of cells in culture from cirrhosis patients. At day 4 spindle shaped cells were observed in cirrhosis but not in control.
Figure 3
Figure 3 Immunocytochemistry of cells in cultures. The cells from control and cirrhosis cultures were immuno-stained with anti CD90 (APC) antibody, immunofluorescent images were captured at 400 × magnification. The cells showed the presence of CD90 (stained in red) and nuclei stained blue.
Figure 4
Figure 4 Colony forming efficiency of cultured cells. A: Cells from control and cirrhosis patients were stained with crystal violet to locate colonies. Colony forming efficiency (CFE) was observed in cirrhosis patients; B: Quantitative representation of colony forming efficiency of cultured cells. There was 12 fold increase in CFE of cirrhosis when compared to control cultures.