Identification of circulating CD90+ CD73+ cells in cirrhosis of liver

Figure 1 Flow cytometry of CD90⁺ CD73⁺ CD45^- cells in peripheral blood cultures. CD90⁺, CD73⁺ CD45^- cells in peripheral blood of control and cirrhosis cultures detected by flow cytometry. After 14 d cultured cells were stained with anti CD90, anti CD73 antibodies and analyzed on FACS Aria II. A: depicts increased number of CD90⁺, CD73⁺ cells in cultures of cirrhosis patients (7.09%) (P < 0.001) compared with controls (0.48%); B: Quantitative representation of the cells in cultures.^bP < 0.001.

Figure 2 Morphological features of cells in cultures. Morphological features of cells in culture from cirrhosis patients. At day 4 spindle shaped cells were observed in cirrhosis but not in control.

Figure 3 Immunocytochemistry of cells in cultures. The cells from control and cirrhosis cultures were immuno-stained with anti CD90 (APC) antibody, immunofluorescent images were captured at 400 × magnification. The cells showed the presence of CD90 (stained in red) and nuclei stained blue.

Figure 4 Colony forming efficiency of cultured cells. A: Cells from control and cirrhosis patients were stained with crystal violet to locate colonies. Colony forming efficiency (CFE) was observed in cirrhosis patients; B: Quantitative representation of colony forming efficiency of cultured cells. There was 12 fold increase in CFE of cirrhosis when compared to control cultures.