Epigenetic states and expression of imprinted genes in human embryonic stem cells

Figure 1 Allele-specific expression of seven imprinted genes. The heterozygous alleles of single nucleotide polymorphism (SNPs) at seven genes, IPW, PEG10, NESP55, KCNQ1, ATP10A, TCEB3C and IGF2 genes, were found by sequencing genomic DNAs (gDNA) of hESC lines T1, T2, T3 and T4 (Table 2). The cDNA sequences of undifferentiated cells and teratomas from hESC lines T1, T2 and/or T3, as well as embryoid bodies of hESC line T4, were determined using either forward (F) or reverse (R) primers given in Table 1. It may be noted that the peak height of heterozygous alleles (nucleotides) was lower than that of homozygous allele (nucleotide) in addition to two different colors instead of single color. When the non-informative homozygotes at SNPs were detected in gDNA, no cDNA sequencing was carried out.