Potential of transposon-mediated cellular reprogramming towards cell-based therapies

doi:10.4252/wjsc.v12.i7.527

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Jul 26, 2020 (publication date) through Apr 26, 2024

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Jul 26, 2020; 12(7): 527-544
Published online Jul 26, 2020. doi: 10.4252/wjsc.v12.i7.527

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Figure 1 Schematic representation of induced pluripotent stem cell derivation, differentiation and genetic modification. iPS: Induced pluripotent stem; CRISPR: Clustered regularly interspaced short palindromic repeats; TALEN: Transcription activator-like endonucleases; ZFN: Zinc finger nucleases.

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Figure 2 Mechanism of action of transposon-transposase mediated transposition. ITRs: Inverted terminal repeats; IR: Inverted repeats; DR: Direct repeats.

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Figure 3 Timeline of transposon-mediated cellular reprogramming of porcine somatic cells to induced pluripotent stem cells (A), change in the morphology of somatic cells in the culture after transposition (B, unpublished own data), timeline of virus mediated cellular reprogramming of somatic cells to induced pluripotent stem cells (C). iPS: Induced pluripotent stem; MEF: Mouse embryonic fibroblast.

Citation: Kumar D, Anand T, Talluri TR, Kues WA. Potential of transposon-mediated cellular reprogramming towards cell-based therapies. World J Stem Cells 2020; 12(7): 527-544
URL: https://www.wjgnet.com/1948-0210/full/v12/i7/527.htm
DOI: https://dx.doi.org/10.4252/wjsc.v12.i7.527