Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background

Figure 1 General strategy for producing gene targeted mice by different Embryonic Stem (ES), chimera methods. 1: Gene targeting of ES cells, followed by selection of the ES cell clones containing the desired mutations; 2A: The ES cells are injected into diploid blastocysts. F0 chimera mice are only partially derived from the modified ES cells and are bred to obtain F1 generation mice that are uniformly heterozygous for the mutation of interest; 2B: The ES cells are injected into tetraploid blastocysts. The F0 generation is fully derived from the gene targeted ES cells.