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Li S, Xiaowen Y, Yang Y, Liu L, Sun Y, Liu Y, Yin L, Chen Z. Osteogenic and anti-inflammatory effect of the multifunctional bionic hydrogel scaffold loaded with aspirin and nano-hydroxyapatite. Front Bioeng Biotechnol 2023; 11:1105248. [PMID: 36761294 PMCID: PMC9902883 DOI: 10.3389/fbioe.2023.1105248] [Citation(s) in RCA: 8] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/22/2022] [Accepted: 01/13/2023] [Indexed: 01/26/2023] Open
Abstract
Although tissue engineering offered new approaches to repair bone defects, it remains a great challenge to create a bone-friendly microenvironment and rebuild bone tissue rapidly by a scaffold with a bionic structure. In this study, a multifunctional structurally optimized hydrogel scaffold was designed by integrating polyvinyl alcohol (PVA), gelatin (Gel), and sodium alginate (SA) with aspirin (ASA) and nano-hydroxyapatite (nHAP). The fabrication procedure is through a dual-crosslinking process. The chemical constitution, crystal structure, microstructure, porosity, mechanical strength, swelling and degradation property, and drug-release behavior of the hydrogel scaffold were analyzed. Multi-hydrogen bonds, electrostatic interactions, and strong "egg-shell" structure contributed to the multi-network microstructure, bone tissue-matched properties, and desirable drug-release function of the hydrogel scaffold. The excellent performance in improving cell viability, promoting cell osteogenic differentiation, and regulating the inflammatory microenvironment of the prepared hydrogel scaffold was verified using mouse pre-osteoblasts (MC3T3-E1) cells. And the synergistic osteogenic and anti-inflammatory functions of aspirin and nano-hydroxyapatite were also verified. This study provided valuable insights into the design, fabrication, and biological potential of multifunctional bone tissue engineering materials with the premise of constructing a bone-friendly microenvironment.
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Affiliation(s)
- Shaoping Li
- Key Laboratory of Stomatology in Hebei Province, Hospital of Stomatology Hebei Medical University, Shijiazhuang, China
| | - Yundeng Xiaowen
- Key Laboratory of Stomatology in Hebei Province, Hospital of Stomatology Hebei Medical University, Shijiazhuang, China
| | - Yuqing Yang
- Key Laboratory of Stomatology in Hebei Province, Hospital of Stomatology Hebei Medical University, Shijiazhuang, China
| | - Libo Liu
- College of Dentistry, Hebei Medical University, Shijiazhuang, China
| | - Yifan Sun
- College of Dentistry, Hebei Medical University, Shijiazhuang, China
| | - Ying Liu
- College of Dentistry, Hebei Medical University, Shijiazhuang, China
| | - Lulu Yin
- College of Dentistry, Hebei Medical University, Shijiazhuang, China
| | - Zhiyu Chen
- Key Laboratory of Stomatology in Hebei Province, Hospital of Stomatology Hebei Medical University, Shijiazhuang, China,*Correspondence: Zhiyu Chen,
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Takalkar S, Girotra C, Kini Y, Padhye M, Tomar G, Acharya S, Gite M. Evaluation of Sexual Dimorphism in Schneider Membrane Thickness Using Cone-Beam Computed Tomography (CBCT) for its Clinical and Forensic Implications. J Maxillofac Oral Surg 2021. [DOI: 10.1007/s12663-021-01531-2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/21/2022] Open
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Buzayan MM, Mahdey HM, Ning CJ. A new approach for better anterior esthetic using platelet-rich fibrin as sole graft material combined with ovate design dental bridge. J Indian Prosthodont Soc 2020; 20:219-223. [PMID: 32655228 PMCID: PMC7335022 DOI: 10.4103/jips.jips_264_19] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/20/2019] [Revised: 09/11/2019] [Accepted: 02/24/2020] [Indexed: 12/19/2022] Open
Abstract
Alveolar ridge deficiency is considered a major esthetic limitation, especially in the maxillary anterior region. Several approaches have been developed to enhance and increase the soft-tissue volume. Among those approaches are connective tissue grafts, platelet-rich fibrin (PRF) membrane and implying the guided bone regeneration concept. The PRF grafting technique was employed in this clinical case to improve and enhance the anterior esthetic without the need for the bone graft and augmentation. This article describes the use of PRF as a sole grafting material for both socket and soft-tissue augmentation for a 23-year-old male, who had an accident 8 years ago while he was playing a basketball, which caused his upper front teeth to be intruded and discolored. The upper left central tooth suffered a major external root resorption; hence, it was extracted. PRF was prepared and packed in and extruded out of the socket. This was combined with ovate design provisional bridge. Ten days, 1 month, 3 months, and 6 months postoperative review showed a significantly well-progressed healing. According to the encouraging result obtained in this clinical case in regard to tissue healing and esthetic, the PRF can be a potential sole graft material for small anterior deficient areas. This may reduce the need of bone augmentation and graft in such selected cases.
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Affiliation(s)
- Muaiyed Mahmoud Buzayan
- Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia
| | - Haydar Majeed Mahdey
- Department of Oral Surgery, Faculty of Dentistry, SEGi University, Petaling Jaya, Malaysia
| | - Chong Jun Ning
- Department of Prosthodontic Dentistry, Faculty of Dentistry, SEGi University, Petaling Jaya, Malaysia
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Guo T, Gulati K, Shen Z, Han P, Fan Z. Therapeutic outcomes of non-grafted and platelet concentrations-grafted transcrestal maxillary sinus elevation (TSFE): a systematic review and meta-analysis. Sci Rep 2020; 10:5935. [PMID: 32245996 PMCID: PMC7125188 DOI: 10.1038/s41598-020-62407-y] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/14/2019] [Accepted: 03/11/2020] [Indexed: 12/28/2022] Open
Abstract
To evaluate and compare the stability, quantity and quality of bone augmentation at maxillary sinus elevation sites by non-grafted transcrestal sinus floor elevation (TSFE) and platelet concentration grafted transcrestal sinus floor elevation (PC-TSFE). A complete literature search was performed up to April 2019. Clinical controlled trials, retrospective cohort studies, and prospective cohort studies were selected based on inclusion criteria. The clinical outcomes were implant survival rate (ISR), marginal/crestal bone loss (MBL/CBL) and endo-sinus bone gain (ESBG). Meta‐analysis was conducted on these 1-year based values. Furthermore, another meta-analysis on 1-year ISR value was conducted among studies with different residual bone heights (RBH) within the non-grafted TSFE group. A total of 18 studies were included: 13 in TSFE group and 5 in PC-TSFE group. No significant differences were displayed between the 1-year ISR of TSFE (97%, 95%CI = 0.96–0.99) and PC-TSFE group (99%, 95%CI = 0.97–1.00). Among the various studies with different RBH within TSFE group, no significant differences in 1-year ISR were displayed. The 1-year MBL/CBL value of PC-TSFE group (0.73 mm, 95%CI = 0.43–1.13 mm) did not show significant difference as compared to TSFE group (0.60 mm, 95%CI = 0.10–1.10 mm). Furthermore, no significant enhancement was observed on 1-year ESBG value on PC-TSFE group (3.51 mm, 95%CI = 2.31–4.71 mm) in comparison with the TSFE group (2.87 mm, 95%CI = 2.18m–3.55 mm). Grafting platelet concentrations around dental implants at TSFE sites did not significantly enhance the adjacent bone regeneration. Moreover, TSFE was shown to be a reliable therapeutic option for implant sites that need simultaneous maxillary sinus augmentation, even under limited RBH.
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Affiliation(s)
- Tianqi Guo
- The University of Queensland, School of Dentistry, Herston, QLD 4006, Australia
| | - Karan Gulati
- The University of Queensland, School of Dentistry, Herston, QLD 4006, Australia
| | - Ziyun Shen
- Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, PR China.,Department of Oral Implant, School and Hospital of Stomatology, Tongji University, Shanghai, 200072, PR China
| | - Pingping Han
- The University of Queensland, School of Dentistry, Herston, QLD 4006, Australia
| | - Zhen Fan
- Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, PR China. .,Department of Oral Implant, School and Hospital of Stomatology, Tongji University, Shanghai, 200072, PR China.
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Solakoglu Ö, Götz W, Kiessling MC, Alt C, Schmitz C, Alt EU. Improved guided bone regeneration by combined application of unmodified, fresh autologous adipose derived regenerative cells and plasma rich in growth factors: A first-in-human case report and literature review. World J Stem Cells 2019; 11:124-146. [PMID: 30842809 PMCID: PMC6397807 DOI: 10.4252/wjsc.v11.i2.124] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 10/09/2018] [Revised: 12/07/2018] [Accepted: 01/10/2019] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND Novel strategies are needed for improving guided bone regeneration (GBR) in oral surgery prior to implant placement, particularly in maxillary sinus augmentation (GBR-MSA) and in lateral alveolar ridge augmentation (LRA). This study tested the hypothesis that the combination of freshly isolated, unmodified autologous adipose-derived regenerative cells (UA-ADRCs), fraction 2 of plasma rich in growth factors (PRGF-2) and an osteoinductive scaffold (OIS) (UA-ADRC/PRGF-2/OIS) is superior to the combination of PRGF-2 and the same OIS alone (PRGF-2/OIS) in GBR-MSA/LRA. CASE SUMMARY A 79-year-old patient was treated with a bilateral external sinus lift procedure as well as a bilateral lateral alveolar ridge augmentation. GBR-MSA/LRA was performed with UA-ADRC/PRGF-2/OIS on the right side, and with PRGF-2/OIS on the left side. Biopsies were collected at 6 wk and 34 wk after GBR-MSA/LRA. At the latter time point implants were placed. Radiographs (32 mo follow-up time) demonstrated excellent bone healing. No radiological or histological signs of inflammation were observed. Detailed histologic, histomorphometric, and immunohistochemical analysis of the biopsies evidenced that UA-ADRC/PRGF-2/OIS resulted in better and faster bone regeneration than PRGF-2/OIS. CONCLUSION GBR-MSA with UA-ADRCs, PRGF-2, and an OIS shows effectiveness without adverse effects.
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Affiliation(s)
- Önder Solakoglu
- External Visiting Lecturer, Dental Department of the University Medical Center Hamburg-Eppendorf, Hamburg 20246, Germany
- Clinic for Periodontology and Implantology, Hamburg 22453, Germany.
| | - Werner Götz
- Department of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn 53111, Germany
| | - Maren C Kiessling
- Institute of Anatomy, Faculty of Medicine, LMU Munich, Munich 80336, Germany
| | | | - Christoph Schmitz
- Institute of Anatomy, Faculty of Medicine, LMU Munich, Munich 80336, Germany
| | - Eckhard U Alt
- InGeneron GmbH, Munich 80331, Germany
- InGeneron, Inc., Houston, TX 77054, United States
- Isar Klinikum Munich, 80331 Munich, Germany
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Elakkiya S, Ramesh AS, Prabhu K. Systematic analysis on the efficacy of bone enhancement methods used for success in dental implants. J Indian Prosthodont Soc 2017; 17:219-225. [PMID: 28936034 PMCID: PMC5601489 DOI: 10.4103/jips.jips_19_17] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/21/2017] [Accepted: 04/10/2017] [Indexed: 01/27/2023] Open
Abstract
Bone grafting is beneficial in enhancing bones that are lost due to trauma or natural or pathologic process. Autogenous bone, allogenic bone, xenogeneic bone, bone substitutes, and alloplasts can also be used for this purpose. Bone quantity should be adequate for the placement of implants, which necessitate the use of bone grafts before implant placement. OBJECTIVE This review analyses the different bone graft materials that are used for grafting around implants and evaluate if these grafts yield successful implant osseointegration over a period of time. MATERIALS AND METHODS The MEDLINE-PubMed database was searched from September 2016 to 10 years previously. Several journals were hand searched and from cross-references. The primary outcome measure that was analyzed was the survival rate of dental implants in the grafted sites at 6 months-1 year, and the secondary outcomes were success rates of dental implants over a period of 3-5 years' follow-up. RESULTS The search yielded 213 articles. Ultimately, 31 studies meeting the eligibility criteria were selected. The analysis shows that autologous bone grafts can be preferred over allografts and xenografts for grafting implant sites, which showed less complication and high success rate. CONCLUSION Based on the available data in the current existing studies with a follow-up period of at least 3-5 years, it can be summarized that the autologous bone grafts can be preferred over allografts and xenografts for grafting implant sites since they are stable for at least 3-5 years.
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Affiliation(s)
- S Elakkiya
- Department of Prosthodontics, Adhiparasakthi Dental College, Melmaruvathur, Tamil Nadu, India
| | - A S Ramesh
- Department of Prosthodontics, Adhiparasakthi Dental College, Melmaruvathur, Tamil Nadu, India
| | - K Prabhu
- Department of Prosthodontics, Adhiparasakthi Dental College, Melmaruvathur, Tamil Nadu, India
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