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Huang LR, Zheng XF, Feng ZR, Wu HK, Mo AC. Hydraulic sinus floor elevation using advanced platelet-rich fibrin: A randomized clinical trial with two-dimensional radiographic results. J Prosthodont Res 2025; 69:275-284. [PMID: 39384385 DOI: 10.2186/jpr.jpr_d_24_00065] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/11/2024]
Abstract
PURPOSE To assess the radiographic outcomes, complications, and implant survival rates of advanced platelet-rich fibrin versus xenografts in hydraulic sinus floor elevation. METHODS In this randomized trial, 40 patients with 46 implants were divided into two groups: a test group (advanced platelet-rich fibrin alone) and a control group (xenograft alone). The key outcome measures included bone regeneration, implant survival, and complications. RESULTS Both groups achieved 100% implant survival. One case of maxillary sinus infection occurred in the control group after surgery. There was no significant difference in bone regeneration between the two groups at 6 months post-surgery and 12 months post-load (P > 0.05). The residual bone height and sinus width at the apex of the implant were significant negative predictors of bone regeneration (P < 0.05), whereas the presence of adjacent teeth was a significant positive predictor (P < 0.05). CONCLUSIONS Both advanced platelet-rich fibrin and xenografts effectively enhanced bone growth at sinus floor elevation, achieving high implant survival rates over one year. Advanced platelet-rich fibrin alone may be a viable xenograft alternative, necessitating further long-term studies to confirm its efficacy. The study was registered in the Chinese Clinical Trial Registry (http://www.chictr.org.cn/) with the registration number ChiCTR2100042060. This clinical trial was not registered before participant recruitment or randomization.
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Affiliation(s)
- Li-Rong Huang
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Implant Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Geriatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China
| | - Xiao-Fei Zheng
- Stomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
| | - Ze-Ru Feng
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Implant Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China
| | - Hong-Kun Wu
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Geriatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China
| | - An-Chun Mo
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Implant Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China
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Egas LS, Matsumoto MA, Hadad H, Tonini KR, de Weert DAB, Perri de Carvalho PS, Ponzoni D. Comparative study of the effect of different temperatures on bovine bone used for bone repair of critical calvaria defects in rats. J Biomed Mater Res B Appl Biomater 2023; 111:1024-1034. [PMID: 36517955 DOI: 10.1002/jbm.b.35211] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/04/2022] [Revised: 11/16/2022] [Accepted: 11/23/2022] [Indexed: 12/16/2022]
Abstract
To evaluate the osteoconductive potential of inorganic biomaterials of bovine origin submitted to different temperatures in the bone repair of critical defects in rat calvaria. Forty-eight rats were divided into four groups according to the material used to fill the defect: control group (GC), the defect was filled only with blood clot (n = 12); GBO, defect filled with Bio-Oss®, deproteinzed at 300°C (n = 12); GOX, defect filled with Inorganic GenOx®, deproteinzed from 850 to 1200°C (n = 12) and G700, defect filled with Inorganic GenOx 700, deproteinzed at 700°C (n = 12). In each animal's calvaria, a trephine bur with 5 mm internal diameter was used to produce a 6 mm-diameter central defect. Gen Derm® resorbable bovine membrane was superimposed over all defects. Subsequently, animals were euthanized at 30 and 60 days after surgery. The pieces were sent for histological and histometric analysis to evaluate the following variables: bone neoformation, presence of biomaterial, mononuclear and polymorphonuclear leukocytes, presence of other tissues (granulation and medullary) and maturation of collagen fibers. The most representative group for bone neoformation was GC. At 30 days, there was a higher mean of mature bone tissue (75.8). At 60 days, there was no statistical difference between the GC (64.9), GBO (32.9), GOX (45.3), and G700 (26.6) groups. GBO presented the highest amount of biomaterial after 30 days (115.9) and 60 days (118.5). All bovine biomaterials were biocompatible and osteoconductive. GOX promoted the best bone repair of the studied materials.
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Affiliation(s)
- Lais Sara Egas
- Department of Diagnosis and Surgery, São Paulo State University (UNESP), Araçatuba School of Dentistry, Brazil., São Paulo, Brazil
| | - Mariza Akemi Matsumoto
- Department of Basic Sciences, State University (UNESP) School of Dentistry, Araçatuba, Brazil
| | - Henrique Hadad
- Department of Diagnosis and Surgery, São Paulo State University (UNESP), Araçatuba School of Dentistry, Brazil., São Paulo, Brazil
| | - Karen Rawen Tonini
- Department of Diagnosis and Surgery, São Paulo State University (UNESP), Araçatuba School of Dentistry, Brazil., São Paulo, Brazil
| | - Daniela Atili Brandini de Weert
- Department of Diagnosis and Surgery, São Paulo State University (UNESP), Araçatuba School of Dentistry, Brazil., São Paulo, Brazil
| | | | - Daniela Ponzoni
- Department of Diagnosis and Surgery, São Paulo State University (UNESP), Araçatuba School of Dentistry, Brazil., São Paulo, Brazil
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Sivakumar I, Arunachalam S, Mahmoud Buzayan M, Sharan J. Does the use of platelet-rich plasma in sinus augmentation improve the survival of dental implants? A systematic review and meta-analysis. J Oral Biol Craniofac Res 2023; 13:57-66. [PMID: 36465890 PMCID: PMC9712696 DOI: 10.1016/j.jobcr.2022.11.002] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/04/2022] [Revised: 11/19/2022] [Accepted: 11/21/2022] [Indexed: 11/25/2022] Open
Abstract
Background Platelet-rich plasma is considered an effective modality to promote bone regeneration, improve hard and soft tissue healing in surgical procedures including sinus augmentation. However, the survival of dental implants in sinus augmented sites with platelet-rich plasma has shown equivocal results in recent studies. Purpose In this systematic review, data on dental implants' survival in sinus augmentation sites with platelet-rich plasma were examined. Materials and methods Randomized controlled trials on the topic with a minimum mean follow-up of 6 months with no language restriction were considered. Other study designs on the topic were excluded. Accordingly, relevant articles were searched in Clinicaltrials.gov, Cochrane databases, PubMed/Medline, and Scopus up to April 2021. Using the Cochrane risk of bias assessment tool, the listed studies' risk of bias was evaluated. From the included studies, the pertinent information was taken and pooled for qualitative and quantitative analysis using R software 4.1.1. Results Six randomized controlled trials involving 188 patients who underwent sinus augmentation with and without platelet-rich plasma, and 781 implants were included for qualitative and quantitative analysis. Four hundred and eleven implants were placed in the intervention group (with platelet-rich plasma) and 370 implants were placed in the control group (without platelet-rich plasma). The pooled estimate (OR 0.84, 95% CI 0.37 to 1.91; I2 = 0%) indicated that there was no statistically significant difference observed between the groups. The test for subgroup differences showed no statistically significant differences between the subgroups (p = 0.45) with no heterogeneity (I2 = 0%). Conclusion The bias associated with selective reporting of outcome data was considered as some concern for bias. This systematic review revealed that the effect of platelet-rich plasma is uncertain on the survival of dental implants.
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Koga T, Nakatani Y, Ohba S, Hara M, Sumita Y, Nagai K, Asahina I. Clinical Safety Assessment of Autologous Freeze-Drying Platelet-Rich Plasma for Bone Regeneration in Maxillary Sinus Floor Augmentation: A Pilot Study. J Clin Med 2021; 10:jcm10081678. [PMID: 33919726 PMCID: PMC8070716 DOI: 10.3390/jcm10081678] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/26/2021] [Revised: 04/02/2021] [Accepted: 04/09/2021] [Indexed: 12/29/2022] Open
Abstract
The purpose of this clinical study is to evaluate the safety and preliminary efficacy of autologous freeze-drying platelet-rich plasma (FD-PRP) on bone regeneration in maxillary sinus floor augmentation as a preliminary pilot study. Five patients that required sinus floor augmentation to facilitate the placement of dental implants participated in this clinical study. The PRP was prepared from the autologous peripheral blood and was lyophilized and stored at −20 °C for 4 weeks before surgery. At surgery, triple-concentrated FD-PRP (x3FD-PRP) mixed with synthetic bone grafting materials was rehydrated following the transplantation into the sinus floor. The primary outcome was a safety verification of x3FD-PRP, evaluated in terms of the clinical course and consecutive blood tests. The secondary outcome was clinical efficacy focused on bone regeneration in sinus floor augmentation evaluated by radiographic examination and implant stability. There were no adverse events, such as systemic complications, excessive inflammatory reactions, severe infection, or local site healing complications, besides those on the usual course associated with surgery. Vertical augmented height was maintained, and the initial stability of implants was achieved post-operatively in 6 months. The results obtained in this study suggest that x3FD-PRP can be used safely for bone engineering in clinical practice. Further studies are required to draw a conclusion concerning the efficacy of x3FD-PRP since this was a pilot study with a single arm and a small sample size.
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Affiliation(s)
- Takamitsu Koga
- Department of Regenerative Oral Surgery, Institute of Biomedical Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan; (T.K.); (Y.N.); (S.O.); (M.H.)
- Department of Dentistry and Oral Surgery, Imaki-ire General Hospital, Kagoshima 892-8502, Japan
| | - Yuya Nakatani
- Department of Regenerative Oral Surgery, Institute of Biomedical Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan; (T.K.); (Y.N.); (S.O.); (M.H.)
| | - Seigo Ohba
- Department of Regenerative Oral Surgery, Institute of Biomedical Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan; (T.K.); (Y.N.); (S.O.); (M.H.)
| | - Masahito Hara
- Department of Regenerative Oral Surgery, Institute of Biomedical Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan; (T.K.); (Y.N.); (S.O.); (M.H.)
| | - Yoshinori Sumita
- Basic and Translational Research Center for Hard Tissue Disease, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan;
| | - Kazuhiro Nagai
- Transfusion and Cell Therapy Unit, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan;
| | - Izumi Asahina
- Department of Regenerative Oral Surgery, Institute of Biomedical Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan; (T.K.); (Y.N.); (S.O.); (M.H.)
- Correspondence: ; Tel.: +81-95-819-7704; Fax: +81-95-819-7705
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Yang SH, Yu WQ, Zhang JJ, Zhou Z, Meng FR, Wang JY, Shi RN, Zhou YM, Zhao JH. Minimally invasive maxillary sinus augmentation with simultaneous implantation on an elderly patient: A case report. World J Clin Cases 2020; 8:6408-6417. [PMID: 33392324 PMCID: PMC7760422 DOI: 10.12998/wjcc.v8.i24.6408] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/24/2020] [Revised: 10/04/2020] [Accepted: 10/13/2020] [Indexed: 02/05/2023] Open
Abstract
BACKGROUND In this case study, a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly patient. We analyzed the cone-beam computed tomography (CBCT) findings to evaluate bone regeneration.
CASE SUMMARY A 65-year-old female with no contraindications for dental implants and loss of maxillary bilateral molars is described. Examination by CBCT showed the available vertical bone height in the bilateral posterior maxilla was 0.5-6.8 mm in the left and 2.8-6.5 mm in the right. The patient underwent a transalveolar approach using platelet-rich fibrin and bone substitute with simulataneous placement of an implant 10 mm in length. Six months post-surgery, the implant showed excellent osseointegration with the bone graft. Thereafter, full-ceramic crowns were fitted. Follow-up at 2 years demonstrated satisfactory prognosis.
CONCLUSION Platelet-rich fibrin and bone substitute can be used to augment the maxillary sinus with a vertical bone height less than 4 mm.
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Affiliation(s)
- Shi-Hui Yang
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
| | - Wan-Qi Yu
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
| | - Jing-Jie Zhang
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
| | - Zhe Zhou
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
| | - Fan-Rong Meng
- Department of Stomatology, Aviation General Hospital, Beijing 100000, China
| | - Jun-Yan Wang
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
| | - Rui-Ning Shi
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
| | - Yan-Min Zhou
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
| | - Jing-Hui Zhao
- Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, Jilin Province, China
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Camacho-Alonso F, Martínez-Ortiz C, Plazas-Buendía L, Mercado-Díaz AM, Vilaplana-Vivo C, Navarro JA, Buendía AJ, Merino JJ, Martínez-Beneyto Y. Bone union formation in the rat mandibular symphysis using hydroxyapatite with or without simvastatin: effects on healthy, diabetic, and osteoporotic rats. Clin Oral Investig 2020; 24:1479-1491. [PMID: 31925587 DOI: 10.1007/s00784-019-03180-9] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/03/2019] [Accepted: 12/19/2019] [Indexed: 12/12/2022]
Abstract
OBJECTIVE The objective is to compare new bone formation in critical defects in healthy, diabetic, and osteoporotic rats filled with hydroxyapatite (HA) alone and HA combined with simvastatin (SV). MATERIALS AND METHODS A total of 48 adult female Sprague-Dawley rats were randomized into three groups (n = 16 per group): Group, 1 healthy; Group 2, diabetics; and Group 3, osteoporotics. Streptozotocin was used to induce type 1 diabetes in Group 2, while bilateral ovariectomy was used to induce osteoporosis in Group 3. The central portion of the rat mandibular symphysis was used as a physiological critical bone defect. In each group, eight defects were filled with HA alone and eight with HA combined with SV. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2). RESULTS In all groups (healthy, diabetics, and osteoporotics), the defects filled with HA + SV presented greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with bone defects treated with HA alone. CONCLUSIONS Combined application of HA and SV improves bone regeneration in mandibular critical bone defects compared with application of HA alone in healthy, diabetic, and osteoporotic rats. CLINICAL RELEVANCE This study might help to patients with osteoporosis or uncontrolled diabetes type 1, but future studies should be done.
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Affiliation(s)
- F Camacho-Alonso
- Department of Oral Surgery, University of Murcia, Murcia, Spain.
| | | | | | | | | | - J A Navarro
- Department of Histology and Pathological Anatomy, University of Murcia, Murcia, Spain
| | - A J Buendía
- Department of Histology and Pathological Anatomy, University of Murcia, Murcia, Spain
| | - J J Merino
- Department of Biochemistry and Molecular Biology, Complutense University of Madrid, Madrid, Spain
| | - Y Martínez-Beneyto
- Department of Preventive and Community Dentistry, University of Murcia, Murcia, Spain
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PRP and BMAC for Musculoskeletal Conditions via Biomaterial Carriers. Int J Mol Sci 2019; 20:ijms20215328. [PMID: 31717698 PMCID: PMC6862231 DOI: 10.3390/ijms20215328] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/16/2019] [Revised: 10/21/2019] [Accepted: 10/21/2019] [Indexed: 02/06/2023] Open
Abstract
Platelet-rich plasma (PRP) and bone marrow aspirate concentrate (BMAC) are orthobiologic therapies considered as an alternative to the current therapies for muscle, bone and cartilage. Different formulations of biomaterials have been used as carriers for PRP and BMAC in order to increase regenerative processes. The most common biomaterials utilized in conjunction with PRP and BMAC clinical trials are organic scaffolds and natural or synthetic polymers. This review will cover the combinatorial strategies of biomaterial carriers with PRP and BMAC for musculoskeletal conditions (MsCs) repair and regeneration in clinical trials. The main objective is to review the therapeutic use of PRP and BMAC as a treatment option for muscle, bone and cartilage injuries.
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Donos N, Dereka X, Calciolari E. The use of bioactive factors to enhance bone regeneration: A narrative review. J Clin Periodontol 2019; 46 Suppl 21:124-161. [DOI: 10.1111/jcpe.13048] [Citation(s) in RCA: 20] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/31/2018] [Revised: 11/08/2018] [Accepted: 12/20/2018] [Indexed: 12/17/2022]
Affiliation(s)
- Nikos Donos
- Centre for Oral Immunobiology & Regenerative Medicine & Centre for Oral Clinical Research (COCR); Institute of Dentistry, Barts & The London School of Medicine & Dentistry; Queen Mary University of London (QMUL); London UK
| | - Xanthippi Dereka
- Centre for Oral Immunobiology & Regenerative Medicine & Centre for Oral Clinical Research (COCR); Institute of Dentistry, Barts & The London School of Medicine & Dentistry; Queen Mary University of London (QMUL); London UK
- Department of Periodontology; School of Dentistry; National and Kapodistrian University of Athens; Athens Greece
| | - Elena Calciolari
- Centre for Oral Immunobiology & Regenerative Medicine & Centre for Oral Clinical Research (COCR); Institute of Dentistry, Barts & The London School of Medicine & Dentistry; Queen Mary University of London (QMUL); London UK
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Solakoglu Ö, Götz W, Kiessling MC, Alt C, Schmitz C, Alt EU. Improved guided bone regeneration by combined application of unmodified, fresh autologous adipose derived regenerative cells and plasma rich in growth factors: A first-in-human case report and literature review. World J Stem Cells 2019; 11:124-146. [PMID: 30842809 PMCID: PMC6397807 DOI: 10.4252/wjsc.v11.i2.124] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 10/09/2018] [Revised: 12/07/2018] [Accepted: 01/10/2019] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND Novel strategies are needed for improving guided bone regeneration (GBR) in oral surgery prior to implant placement, particularly in maxillary sinus augmentation (GBR-MSA) and in lateral alveolar ridge augmentation (LRA). This study tested the hypothesis that the combination of freshly isolated, unmodified autologous adipose-derived regenerative cells (UA-ADRCs), fraction 2 of plasma rich in growth factors (PRGF-2) and an osteoinductive scaffold (OIS) (UA-ADRC/PRGF-2/OIS) is superior to the combination of PRGF-2 and the same OIS alone (PRGF-2/OIS) in GBR-MSA/LRA. CASE SUMMARY A 79-year-old patient was treated with a bilateral external sinus lift procedure as well as a bilateral lateral alveolar ridge augmentation. GBR-MSA/LRA was performed with UA-ADRC/PRGF-2/OIS on the right side, and with PRGF-2/OIS on the left side. Biopsies were collected at 6 wk and 34 wk after GBR-MSA/LRA. At the latter time point implants were placed. Radiographs (32 mo follow-up time) demonstrated excellent bone healing. No radiological or histological signs of inflammation were observed. Detailed histologic, histomorphometric, and immunohistochemical analysis of the biopsies evidenced that UA-ADRC/PRGF-2/OIS resulted in better and faster bone regeneration than PRGF-2/OIS. CONCLUSION GBR-MSA with UA-ADRCs, PRGF-2, and an OIS shows effectiveness without adverse effects.
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Affiliation(s)
- Önder Solakoglu
- External Visiting Lecturer, Dental Department of the University Medical Center Hamburg-Eppendorf, Hamburg 20246, Germany
- Clinic for Periodontology and Implantology, Hamburg 22453, Germany.
| | - Werner Götz
- Department of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn 53111, Germany
| | - Maren C Kiessling
- Institute of Anatomy, Faculty of Medicine, LMU Munich, Munich 80336, Germany
| | | | - Christoph Schmitz
- Institute of Anatomy, Faculty of Medicine, LMU Munich, Munich 80336, Germany
| | - Eckhard U Alt
- InGeneron GmbH, Munich 80331, Germany
- InGeneron, Inc., Houston, TX 77054, United States
- Isar Klinikum Munich, 80331 Munich, Germany
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An in vitro long-term study of cryopreserved umbilical cord blood-derived platelet-rich plasma containing growth factors-PDGF-BB, TGF-β, and VEGF. J Craniomaxillofac Surg 2019; 47:668-675. [PMID: 30738636 DOI: 10.1016/j.jcms.2019.01.020] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/01/2018] [Revised: 11/20/2018] [Accepted: 01/15/2019] [Indexed: 11/22/2022] Open
Abstract
PURPOSE Umbilical cord blood-derived platelet-rich plasma (UCB-PRP) containing growth factors has attracted attention as a biomaterial useful for regenerative medicine. The osteoblastic differentiation of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) can be induced by UCB-PRP. MATERIALS AND METHODS Nine samples of UC and UCB were used to conduct an in vitro study that determined the contents of three growth factors (i.e., platelet-derived growth factor, transforming growth factor β-1, and vascular endothelial growth factor) and that examined, by staining with Alizarin red, their ability to induce the osteoblastic differentiation of UC-MSCs at the baseline, 3 months, and 3 years of cryopreservation. RESULTS The contents of growth factors in cryopreserved UCB-PRP were markedly elevated compared to those found in UCB at baseline. The samples of UCB that were added with cryopreserved UCB-PRP and those with bone morphogenetic protein-2 were stained granularly with Alizarin red, thus indicating the presence of calcium. The samples of UCB that were not added with UCB-PRP were not stained with Alizarin red. The above-mentioned contents and ability were maintained at 3 years of cryopreservation. Cryopreserved UCB-PRP possibly and advantageously induced the osteoblastic differentiation of UC-MSCs. CONCLUSION The potential clinical application of cryopreserved UCB-PRP to regenerative medicine was suggested.
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Long-Term Implant Survival After 100 Maxillary Sinus Augmentations Using Plasma Rich in Growth Factors. IMPLANT DENT 2018; 26:199-208. [PMID: 28207599 DOI: 10.1097/id.0000000000000561] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
PURPOSE To identify determinants of long-term implant survival after sinus augmentation procedure (SAP) using a combination of plasma rich in growth factors (PRGF) and graft material. MATERIALS AND METHODS Patients were included in this retrospective study if they underwent SAP through a lateral wall approach using PRGF in combination with mainly xenograft, and received implants restored for at least 1 year. Sociodemographic, behavioral and implant characteristics (clinical factors, implant details, surgical details, prosthesis details, and complications) were analyzed. RESULTS Sixty-seven patients received 217 implants in 100 augmented sinuses. The mean follow-up was 7.2 years. Overall, 22 implants failed in 15 patients (90% cumulative implant survival). Multivariable models showed greater implant survival with implants placed with ≥5 mm of residual crestal bone height (RBH) compared with those placed with <3 mm of RBH. There was also an improvement in survival comparing implant lengths. In addition, immediate loading of implants significantly increased the risk of implant failure. CONCLUSION The use of PRGFs for maxillary sinus grafting may be an effective and safe treatment option for the rehabilitation of atrophic edentulous posterior maxillae. However, randomized clinical trials are needed to confirm these findings.
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Ceccarelli G, Presta R, Lupi SM, Giarratana N, Bloise N, Benedetti L, Cusella De Angelis MG, Rodriguez Y Baena R. Evaluation of Poly(Lactic-co-glycolic) Acid Alone or in Combination with Hydroxyapatite on Human-Periosteal Cells Bone Differentiation and in Sinus Lift Treatment. Molecules 2017; 22:molecules22122109. [PMID: 29207466 PMCID: PMC6149689 DOI: 10.3390/molecules22122109] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/06/2017] [Revised: 11/27/2017] [Accepted: 11/27/2017] [Indexed: 01/03/2023] Open
Abstract
Most recent advances in tissue engineering in the fields of oral surgery and dentistry have aimed to restore hard and soft tissues. Further improvement of these therapies may involve more biological approaches and the use of dental tissue stem cells in combination with inorganic/organic scaffolds. In this study, we analyzed the osteoconductivity of two different inorganic scaffolds based on poly (lactic-co-glycolic) acid alone (PLGA-Fisiograft) or in combination with hydroxyapatite (PLGA/HA-Alos) in comparison with an organic material based on equine collagen (PARASORB Sombrero) both in vitro and in vivo. We developed a simple in vitro model in which periosteum-derived stem cells were grown in contact with chips of these scaffolds to mimic bone mineralization. The viability of cells and material osteoconductivity were evaluated by osteogenic gene expression and histological analyses at different time points. In addition, the capacity of scaffolds to improve bone healing in sinus lift was examined. Our results demonstrated that the osteoconductivity of PLGA/HA-Alos and the efficacy of scaffolds in promoting bone healing in the sinus lift were increased. Thus, new clinical approaches in sinus lift follow-up should be considered to elucidate the clinical potential of these two PLGA-based materials in dentistry.
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Affiliation(s)
- Gabriele Ceccarelli
- Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, 27100 Pavia, Italy.
- Center for Health Technologies, University of Pavia, 27100 Pavia, Italy.
| | - Rossella Presta
- Department of Clinico-Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, University of Pavia, P.le Golgi 2, 27100 Pavia, Italy.
| | - Saturnino Marco Lupi
- Department of Clinico-Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, University of Pavia, P.le Golgi 2, 27100 Pavia, Italy.
| | - Nefele Giarratana
- Department of Development and Regeneration, Laboratory of Translational Cardiomyology, KU Leuven, B-3000 Leuven, Belgium.
| | - Nora Bloise
- Center for Health Technologies, University of Pavia, 27100 Pavia, Italy.
- Molecular Medicine Department (DMM), Center for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli 3/B, 27100 Pavia, Italy.
- Department of Occupational Medicine, Toxicology and Environmental Risks, Istituti Clinici Scientifici Maugeri S.p.A, IRCCS, Via S. Boezio 28, 27100 Pavia, Italy.
| | - Laura Benedetti
- Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, 27100 Pavia, Italy.
- Center for Health Technologies, University of Pavia, 27100 Pavia, Italy.
| | - Maria Gabriella Cusella De Angelis
- Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, 27100 Pavia, Italy.
- Center for Health Technologies, University of Pavia, 27100 Pavia, Italy.
| | - Ruggero Rodriguez Y Baena
- Department of Clinico-Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, University of Pavia, P.le Golgi 2, 27100 Pavia, Italy.
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Meyer J, Balaphas A, Fontana P, Sadoul K, Morel P, Gonelle‐Gispert C, Bühler L. Platelets in liver regeneration. ISBT SCIENCE SERIES 2017; 12:455-462. [DOI: 10.1111/voxs.12382] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Abstract
Background and ObjectivesLoss of liver tissue leading to impairment of liver function represents a major cause of mortality. Understanding the mechanism of liver regeneration and developing therapies to sustain liver regeneration are of high therapeutic relevance. In this regard, platelets are considered as potential candidates for stimulating liver regeneration.MethodsWe aim to review the most recent evidence regarding the role of platelets in liver regeneration.ResultsPlatelets stimulate liver regeneration in animal models of liver resection. In humans, platelets are independent predictors of postoperative mortality, liver function and volume recovery. One proposed mechanism by which platelets stimulate liver regeneration relies on their direct effect on hepatocytes. Following partial hepatectomy, platelets accumulate in the residual liver and release their granule content. Platelet‐containing molecules, such as HGF, VEGF, IGF‐1 and serotonin, stimulate hepatocyte proliferation. A putative additional mechanism involves the transfer of platelet mRNA to hepatocytes following platelet internalization. Recent studies have suggested that the effect of platelets relies on their interactions with LSEC. Platelets induce the secretion of IL‐6 from LSEC, a strong initiator of hepatocyte proliferation. Additionally, platelets convey molecules that may impact LSEC function and, by extension, liver regeneration. Platelets potentially interact with Kupffer cells, but the effect of that interaction on liver regeneration remains to be determined.ConclusionPlatelets stimulate liver regeneration. Several mechanisms seem to be involved, acting on the level of hepatocytes, LSEC and potentially Kupffer cells. Identification of the platelet‐molecule(s) involved may lead to targeted therapies for patients with impairment of liver function.
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Affiliation(s)
- J. Meyer
- Division of Digestive and Transplantation Surgery University Hospitals of Geneva Genève Switzerland
- Unit of Surgical Research University of Geneva Genève Switzerland
| | - A. Balaphas
- Division of Digestive and Transplantation Surgery University Hospitals of Geneva Genève Switzerland
- Unit of Surgical Research University of Geneva Genève Switzerland
| | - P. Fontana
- Division of Angiology and Haemostasis University Hospitals of Geneva Genève Switzerland
- Geneva Platelet Group University of Geneva Genève Switzerland
| | - K. Sadoul
- Regulation and pharmacology of the cytoskeleton Institute for Advanced Biosciences Université Grenoble Alpes Grenoble France
| | - P. Morel
- Division of Digestive and Transplantation Surgery University Hospitals of Geneva Genève Switzerland
- Unit of Surgical Research University of Geneva Genève Switzerland
| | | | - L. Bühler
- Division of Digestive and Transplantation Surgery University Hospitals of Geneva Genève Switzerland
- Unit of Surgical Research University of Geneva Genève Switzerland
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Park CG, Joo MW, Jeong J, Kang YK, Lee DR. Evaluation of the effects of the combination of autologous mesenchymal stem cells and platelet-rich plasma on structural bone allograft healing. Cell Tissue Bank 2017; 18:229-238. [DOI: 10.1007/s10561-017-9611-z] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/06/2016] [Accepted: 02/14/2017] [Indexed: 10/20/2022]
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Effect of Ratio of Residual Alveolar Bone to Graft Material in Contact With Fixture Surface on Marginal Bone Loss of Implants in Augmented Maxillary Sinuses: A 1-Year Retrospective Study. IMPLANT DENT 2017; 26:80-86. [PMID: 28114242 DOI: 10.1097/id.0000000000000539] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/27/2022]
Abstract
PURPOSE The purpose of this retrospective study was to evaluate the influences of height or area ratio of residual alveolar bone to graft material on marginal bone loss around implants in the augmented maxillary sinuses with delayed implant placement. MATERIALS AND METHODS In this study, 42 patients with Astra implants in sinuses that had been augmented with alloplasts and allografts or xenografts (alveolar bone height ≤ 5 mm) were selected. Marginal bone level surrounding 1 implant per sinus was assessed by radiographic imaging at the time of final restoration delivery and 12 months after functional loading. To evaluate the marginal bone level alterations using clinical and radiographic data, Pearson's correlation analysis and Mann-Whitney test were performed. RESULTS Forty-six implants were included in this study. The residual bone/implant length ratio and the residual bone/implant area ratio were not associated with marginal bone loss at 1 year after functional loading (P > 0.05). And, marginal bone loss did not differ significantly between 2 types of graft materials during the observation period (P > 0.05). CONCLUSION The residual bone/implant length ratio and residual bone/implant area ratio were not associated with marginal bone loss around implants placed in augmented sinuses during 1 year of functional loading.
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Nakatani Y, Agata H, Sumita Y, Koga T, Asahina I. Efficacy of freeze-dried platelet-rich plasma in bone engineering. Arch Oral Biol 2016; 73:172-178. [PMID: 27771585 DOI: 10.1016/j.archoralbio.2016.10.006] [Citation(s) in RCA: 28] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/11/2016] [Revised: 09/05/2016] [Accepted: 10/11/2016] [Indexed: 11/19/2022]
Abstract
OBJECTIVE Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. DESIGN FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at -20°C for one month. It was then rehydrated with an equal or 1/3 amount of distilled water (×1FD-PRP, ×3FD-PRP, respectively), and we assessed its gelation properties and the release of growth factors (PDGF-BB, TGF-β1, and VEGF). We also examined the bone forming ability with onlay-grafting on mice calvaria using β-TCP granules as a scaffold. RESULTS FD-PRP showed comparable gelation as f-PRP. In terms of growth factor release,×1FD-PRP released identical concentrations of PDGF-BB and TGF-β1 to f-PRP, while ×3FD-PRP released approximately 3-fold concentrations when compared with f-PRP. In vivo, ×1FD-PRP promoted identical levels of the bone formation as f-PRP, and ×3FD-PRP induced more abundant bone formation. CONCLUSIONS These results suggest that f-PRP can be stored without functional loss by freeze-drying and the concentration of PRP may improve its efficacy in bone engineering.
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Affiliation(s)
- Yuya Nakatani
- Department of Regenerative Oral Surgery, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, Japan
| | - Hideki Agata
- Department of Regenerative Oral Surgery, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, Japan
| | - Yoshinori Sumita
- Department of Regenerative Oral Surgery, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, Japan
| | - Takamitsu Koga
- Department of Regenerative Oral Surgery, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, Japan
| | - Izumi Asahina
- Department of Regenerative Oral Surgery, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-shi, Nagasaki 852-8102, Japan.
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Jackson RF. Using Platelet-Rich Plasma to Promote Healing and Prevent Seroma Formation in Abdominoplasty Procedures. ACTA ACUST UNITED AC 2016. [DOI: 10.1177/074880680302000402] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
Abstract
Introduction: To evaluate the efficacy of platelet-rich plasma in promoting wound healing and preventing seroma formation, a 30-patient study was conducted between January 2001 and May 2003. The intent of the study was to evaluate the use of platelet-rich concentrate on patients undergoing either full abdominoplasty (4 patients) or crescent tuck abdominoplasty (miniabdominoplasty) surgery. This series of patients was contrasted with a 100-patient study with similar surgery accomplished between 1990 and 2000 and previously reported at the 17th International Congress of the French Society of Aesthetic Surgery and the 5th International Congress of the Society of Lipo-Plasty. In addition, during the 30-patient study, there were 6 patients studied who did not receive platelet-rich concentrate. Those 6 patients were also compared with the 30-patient platelet-concentrate group. Materials and Methods: The platelet concentrate was prepared from 54 mL of blood drawn from the patient at the time of surgery. The anticoagulated volume of 60 mL was processed using a uniquely designed centrifuge system manufactured by Harvest Technologies (Plymouth, Mass). The separation process yielded 10 mL of platelet-rich plasma (PRP). This volume was combined with 1 mL of calcium thrombin in a spray that was applied to the surgical site. The calcium thrombin was used to activate the platelets and fibrinogen to form fibrin chains in order to achieve an immediate hemostatic seal on the tissue beds. The calcium thrombin was prepared by adding 5 mL of 10% calcium chloride to 5000 units of bovine thrombin. Only 1 mL of this mixture was used in the procedure. Prior to the application of the platelet concentrate/calcium thrombin to the tissue beds, absolute hemostasis is accomplished and both beds are blotted as dry as possible. Both the top and bottom layer are sprayed with approximately 4.5 mL of platelet concentrate/calcium thrombin. The tissue layers were approximated and the skin surface rolled to express any fluid from the location of the sutures. Sutures were then placed and the remaining PRP/calcium thrombin mixture was sprayed along the suture line. The formal abdominoplasty was done without liposuction in the upper or epigastric area of the abdomen. The crescent tuck abdominoplasty used was the same as the previous 100-patient series. Results: No seromas were found in the 30 patients studied as compared with a 7% seroma rate in the previous 100-patient series and 2 out of 6 of the patients who had abdominoplasty during the same time as the 30-patient group developed seromas. Anecdotally, the wounds seemed to heal much more rapidly with more esthetically pleasing incisions. Discussion: The addition of platelet-rich plasma is now easily applied at the surgical site with a very easily usable technology that does not require expensive or complicated equipment. The application of platelet-rich plasma as a natural fibrin matrix delivers growth factors to the wound and seems to promote more rapid healing.
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Affiliation(s)
- Robert F. Jackson
- From the Ambulatory Care Center, 330 North Wabash Avenue, Marion, Ind
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Danesh-Sani SA, Movahed A, ElChaar ES, Chong Chan K, Amintavakoli N. Radiographic Evaluation of Maxillary Sinus Lateral Wall and Posterior Superior Alveolar Artery Anatomy: A Cone-Beam Computed Tomographic Study. Clin Implant Dent Relat Res 2016; 19:151-160. [PMID: 27238049 DOI: 10.1111/cid.12426] [Citation(s) in RCA: 42] [Impact Index Per Article: 4.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
Abstract
OBJECTIVE The purpose of the current study is to assess the thickness of the maxillary sinus lateral wall in dentate and edentulous patients using cone beam computed tomography (CBCT). This study also provides information about the diameter, prevalence, and course of the posterior superior alveolar artery (PSAA), and its relation to the maxillary sinus floor. MATERIALS AND METHODS Four hundred and thirty CBCT scans of the maxillofacial complex (860 maxillary sinuses) were reviewed. Measurements of the lateral wall of the maxillary sinus and PSAA were performed on the CBCT images. RESULTS Statistical analysis showed that dental status (edentulous, non-edentulous) of the patients had no significant effect on the lateral wall thickness. The mean thickness of the lateral wall of the maxillary sinus was 1.21 ± 1.07 mm at the second molar (M2), 1.98 ± 1.87 mm at the first molar (M1), 2.02 ± 1.53 mm at the second premolar (P2) and 2.16 ± 1.25 mm at the first premolar (P1). There was statistically significant difference between the left and right sides of the maxillary sinus only at P2 (p =.043). Detection rate of the PSAA on CBCT was reported as 60.58%. The mean diameter of the artery was 1.17 mm (range 0.4-2.8 mm). There was no significant correlation between age and the size of the PSAA. The most frequent path of the PSAA was intraosseous (69.6%), followed by intrasinusal (24.3%) and superficial (6.1%). The overall mean distance of the PSAA from the floor of the maxillary sinus is 8.16 mm. CONCLUSIONS The results from this study suggest that using CBCT prior to the surgery provides valuable diagnostic information. However, undetected intraosseous canal in CBCT does not exclude its existence. Alteration in the lateral window design and the use of piezoelectric instruments are recommended if intraoperative complications are expected.
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Affiliation(s)
- Seyed Amir Danesh-Sani
- Department of Periodontology and Implant Dent, New York University College of Dentistry, New York, NY, USA
| | - Ali Movahed
- Avicenna Research Institute, Dental Research Center, Oral Surgery Division, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Edgard S ElChaar
- Program director of advanced program in periodontology and Implant Dent, Department of Periodontology and Implant Dent, New York University College of Dentistry, New York, NY, USA
| | - King Chong Chan
- Program director, Department of Oral and Maxillofacial Radiology, New York University College of Dentistry, New York, NY, USA
| | - Niloufar Amintavakoli
- Clinical associate professor, Department of Oral and Maxillofacial Radiology, New York University College of Dentistry, New York, NY, USA
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A Novel HA/β-TCP-Collagen Composite Enhanced New Bone Formation for Dental Extraction Socket Preservation in Beagle Dogs. MATERIALS 2016; 9:ma9030191. [PMID: 28773317 PMCID: PMC5456700 DOI: 10.3390/ma9030191] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/09/2015] [Revised: 03/07/2016] [Accepted: 03/09/2016] [Indexed: 01/22/2023]
Abstract
Past studies in humans have demonstrated horizontal and vertical bone loss after six months following tooth extraction. Many biomaterials have been developed to preserve bone volume after tooth extraction. Type I collagen serves as an excellent delivery system for growth factors and promotes angiogenesis. Calcium phosphate ceramics have also been investigated because their mineral chemistry resembles human bone. The aim of this study was to compare the performance of a novel bioresorbable purified fibrillar collagen and hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) ceramic composite versus collagen alone and a bovine xenograft-collagen composite in beagles. Collagen plugs, bovine graft-collagen composite and HA/β-TCP-collagen composite were implanted into the left and right first, second and third mandibular premolars, and the fourth molar was left empty for natural healing. In total, 20 male beagle dogs were used, and quantitative and histological analyses of the extraction ridge was done. The smallest width reduction was 19.09% ± 8.81% with the HA/β-TCP-collagen composite at Week 8, accompanied by new bone formation at Weeks 4 and 8. The HA/β-TCP-collagen composite performed well, as a new osteoconductive and biomimetic composite biomaterial, for socket bone preservation after tooth extraction.
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Lemos CAA, Mello CC, dos Santos DM, Verri FR, Goiato MC, Pellizzer EP. Effects of platelet-rich plasma in association with bone grafts in maxillary sinus augmentation: a systematic review and meta-analysis. Int J Oral Maxillofac Surg 2016; 45:517-25. [PMID: 26775635 DOI: 10.1016/j.ijom.2015.07.012] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/05/2015] [Revised: 07/06/2015] [Accepted: 07/15/2015] [Indexed: 01/13/2023]
Abstract
This systematic review evaluated the effect on bone formation and implant survival of combining platelet-rich plasma (PRP) with bone grafts in maxillary augmentation. A comprehensive review of articles listed in the PubMed/MEDLINE, Embase, and Cochrane Library databases covering the period January 2000 to January 2015 was performed. The meta-analysis was based on bone formation for which the mean difference (MD, in millimetres) was calculated. Implant survival was assessed as a dichotomous outcome and evaluated using the risk ratio (RR) with 95% confidence interval (CI). The search identified 3303 references. After inclusion and exclusion criteria were applied, 17 studies were selected for qualitative analysis and 13 for quantitative analysis. A total of 369 patients (mean age 51.67 years) and 621 maxillary sinus augmentations were evaluated. After the data analysis, additional analyses were performed of the implant stability quotient, marginal bone loss, and alveolar bone height measured by MD. The results showed no significant difference in implant stability (P=0.32, MD 1.00, 95% CI -0.98 to 2.98), marginal bone loss (P=0.31, MD 0.06, 95% CI -0.05 to 0.16), alveolar bone height (P=0.10, MD -0.72, 95% CI -1.59 to 0.14), implant survival (P=0.22, RR 1.95, 95% CI 0.67-5.69), or bone formation (P=0.81, MD -0.63, 95% CI -5.91 to 4.65). In conclusion, the meta-analysis indicates no influence of PRP with bone graft on bone formation and implant survival in maxillary sinus augmentation.
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Affiliation(s)
- C A A Lemos
- Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP - Universidade Estadual Paulista, Araçatuba, Brazil
| | - C C Mello
- Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP - Universidade Estadual Paulista, Araçatuba, Brazil
| | - D M dos Santos
- Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP - Universidade Estadual Paulista, Araçatuba, Brazil
| | - F R Verri
- Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP - Universidade Estadual Paulista, Araçatuba, Brazil
| | - M C Goiato
- Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP - Universidade Estadual Paulista, Araçatuba, Brazil
| | - E P Pellizzer
- Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP - Universidade Estadual Paulista, Araçatuba, Brazil.
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Radiographic evaluation of regenerated bone following poly(lactic-co-glycolic) acid/hydroxyapatite and deproteinized bovine bone graft in sinus lifting. J Craniofac Surg 2015; 24:845-8. [PMID: 23714894 DOI: 10.1097/scs.0b013e31827ca01a] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023] Open
Abstract
Although numerous biomaterials are used for maxillary sinus-lift surgery, the ideal material for such procedures has not yet been identified. Both heterologous and alloplastic bone substitutes have a solely osteoconductive effect and lack the osteoinductive properties of the bone morphogenetic proteins typical of autologous bone. Our group assessed a new alloplastic graft material, poly(lactic-co-glycolic) acid/hydroxyapatite (PLGA/HA), implanted in a human model of maxillary sinus-lift surgery. For this prospective, random, double-blind trial, we used deproteinized bovine bone (DBB) as the comparison material. Radiographic bone vertical height and density were assessed at approximately 28 weeks after grafting using cone-beam computed tomography. The vertical dimension of the regenerated bone was equivalent between the 2 groups. The density of the bone regenerated using PLGA/HA was significantly lower than that obtained with DBB. Despite clinical assessments demonstrating that PLGA/HA has sufficient characteristics for use in sinus-lift surgery, DBB provided greater bone density and an equivalent vertical dimension of grafted bone. Further studies are needed to supplement the radiologic findings with histologic and micromorphometric examinations.
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Casap N, Laviv A, Debecco M, Alterman M, Laster Z, Jensen OT. Imperforate Titanium Shell Enclosing Recombinant Human Bone Morphogenetic Protein-2–Induced Bone Formation for High-Profile Dental Implants in Rabbit Tibia. J Oral Maxillofac Surg 2015; 73:245-52. [DOI: 10.1016/j.joms.2014.10.002] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/30/2013] [Revised: 10/06/2014] [Accepted: 10/06/2014] [Indexed: 10/24/2022]
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Uzbek UH, Rahman SA, Alam MK, Gillani SW. Bone Forming Potential of An-Organic Bovine Bone Graft: A Cone Beam CT study. J Clin Diagn Res 2014; 8:ZC73-6. [PMID: 25654037 DOI: 10.7860/jcdr/2014/8557.5352] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/19/2014] [Accepted: 05/14/2014] [Indexed: 11/24/2022]
Abstract
PURPOSE An-organic bovine bone graft is a xenograft with the potential of bone formation. The aim of this study was to evaluate the bone density using cone beam computed tomography scans around functional endosseous implant in the region of both augmented maxillary sinus with the an-organic bovine bone graft and the alveolar bone over which the graft was placed to provide space for the implants. MATERIALS AND METHODS Sterile freeze dried bovine bone graft produced by National Tissue Bank, University Sains, Malaysia was used for stage-1 implant placement with maxillary sinus augmentation in a total of 19 subjects with 19 implants. The age of all subjects ranged between 40-60 years with a mean age 51±4.70. All subjects underwent a follow up CT scan using PlanmecaPromax 3D(®) Cone beam computed tomography scanner at the Radiology department, Hospital University Sains, Malaysia. The collected data was then analysed to evaluate bone density in Hounsfield Units using PlanmecaRomexis" Imaging Software 2.2(®) which is specialized accompanying software of the cone beam computed tomography machine. RESULTS There was bone formation seen at the site of the augmented sinus. A significant increase (p<0.005) in bone density was reported at the augmented site compared to the bone density of the existing alveolar bone. CONCLUSION An-organic bovine bone graft is an osteoconductive material that can be used for the purpose of maxillary sinus augmentation.
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Affiliation(s)
- Usman Haider Uzbek
- Masters Student, Department of Oral and Maxillo-facial Surgery, University Sains Malaysia , Kelantan, Malaysia
| | - Shaifulizan Ab Rahman
- Senior Lecturer, Department of Oral and Maxillo-facial Surgery, University Sains Malaysia , Kelantan, Malaysia
| | | | - Syed Wasif Gillani
- Senior Lecturer, Discipline of Pharmacy, Monash University Malaysia , Selangor, Malaysia
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Dikicier S, Dikicier E, Karacayli U. Maxillary sinus augmentation and implant placement using venous blood without graft material: a case letter. J ORAL IMPLANTOL 2014; 40:615-8. [PMID: 25295892 DOI: 10.1563/aaid-joi-d-12-00125] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Affiliation(s)
- Sibel Dikicier
- 1 Department Of Prosthodontics, Corlu Military Hospital, Tekirdag, Turkey
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Bortoluzzi MC, Manfro R, Fabris V, Cecconello R, Derech ED. Comparative study of immediately inserted dental implants in sinus lift: 24 months of follow-up. Ann Maxillofac Surg 2014; 4:30-3. [PMID: 24987595 PMCID: PMC4073458 DOI: 10.4103/2231-0746.133071] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022] Open
Abstract
OBJECTIVES The primary aim of this study is to evaluate the success rate of immediately inserted dental implants in sinus lift and the secondary aim is to assess the reliability of performing simultaneous surgery for sinus lift, grafting and implant placement in situations of very low residual bone heights (<4 mm) in the posterior maxillae. MATERIALS AND METHODS A series of consecutive patients who sought dental implant rehabilitation were included in this study. The sinus lift cases were selected and divided into two groups based on the maxillary alveolar height, ≥4 mm of radiographically measurable bone height (Group 1) and < 4 mm of bone height (Group 2). RESULTS A total of 13 implants were installed in Group 1, whereas 8 implants were installed in Group 2. The success rate for dental implants in the sinus lifts reached 95.2% after a period of 24 months of evaluation, at 100% for Group 1 (bone height ≥ 4 mm) and 87.5% for Group 2 (bone height with < 4 mm). Besides the differences between the percentages of success, statistical differences were not reached when the groups were compared (Fisher's exact test, P = 0.38). CONCLUSIONS Despite the limitations of this study, including its low number of participants, the results indicate that simultaneous surgery for sinus lifting, grafting and dental implants in posterior maxillae with very low bone heights (<4 mm) can be performed safely, although with lower success rates than found in patients with higher residual bone heights (>4 mm).
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Affiliation(s)
- Marcelo Carlos Bortoluzzi
- Department of Dentistry, Dentistry Postgraduate Program, School of Dentistry, Ponta Grossa State University, Paraná, Brazil
| | - Rafael Manfro
- Department of Dentistry, Dentistry Postgraduate Program, School of Dentistry, Ponta Grossa State University, Paraná, Brazil
| | - Vinícius Fabris
- Department of Dentistry, Dentistry Postgraduate Program, School of Dentistry, Ponta Grossa State University, Paraná, Brazil
| | - Rodrigo Cecconello
- Department of Dentistry, Dentistry Postgraduate Program, School of Dentistry, Ponta Grossa State University, Paraná, Brazil
| | - Estevo D'Agostini Derech
- Department of Dentistry, Dentistry Postgraduate Program, School of Dentistry, Ponta Grossa State University, Paraná, Brazil
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Weak link of tendon-bone healing and a control experiment to promote healing. Arch Orthop Trauma Surg 2013; 133:1533-41. [PMID: 23949374 DOI: 10.1007/s00402-013-1802-x] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/30/2012] [Indexed: 02/09/2023]
Abstract
OBJECTIVE This study aims to observe the mechanical weak point and histological features of tendon-bone interface after anterior cruciate ligament reconstructive surgery and to explore the tendon-bone healing effects of the platelet-rich gel (PRG) + deproteinized bone (DPB) compound. METHODS A total of 48 New Zealand white rabbits were randomly divided into normal group, model (without embedding), experimental (embedded with the PRG + DPB compound), and control (embedded with DPB) groups. The rabbits were executed at 2, 4, 8, and 12 weeks after the operation. Then, micro-computed tomography scan and uniaxial tensile test were conducted. The fractured specimens were subjected to histological observation. RESULTS At 4, 8, and 12 weeks after the operation, the bone density of the tendon-bone bound section of the experimental group was higher than that of the other groups (P < 0.05). At 4 and 8 weeks, the maximum tensile load of the experimental group was obviously higher than that of the control and model groups (P < 0.05). Histological observation indicated that the tendon-bone interface in the experimental group had more cartilage and bone tissue growing toward the internal tendon, but the fracture layer mainly occurred in the non-ankylosed part. CONCLUSION The mechanical weak point of the early tendon-bone interface was in the immature fibrous tissue. The PRG + DPB compound can effectively trigger tendon-bone healing by promoting the maturation and ossification of the tendon-bone tissue. This compound improved the tensile strength of the healing interface and reduced bone tunnel enlargement.
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Duttenhoefer F, Souren C, Menne D, Emmerich D, Schön R, Sauerbier S. Long-term survival of dental implants placed in the grafted maxillary sinus: systematic review and meta-analysis of treatment modalities. PLoS One 2013; 8:e75357. [PMID: 24058679 PMCID: PMC3776785 DOI: 10.1371/journal.pone.0075357] [Citation(s) in RCA: 25] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/29/2013] [Accepted: 08/12/2013] [Indexed: 12/23/2022] Open
Abstract
BACKGROUND A prevalent modality to increase the amount of available bone prior to implantation is grafting of the maxillary sinus. Multiple factors such as the surgical technique, moment of implant placement as well as grafting materials and membranes are known to affect implant survival. However, the role of different factor combinations and associated reciprocal effects remain unclear. Conventional statistical methods do not consider inconsistency of study designs and do not take covariables into account. Hence, a systematic research and meta-analysis was conducted to investigate the influence of various treatment modalities on implant survival in the grafted maxillary sinus. MATERIALS AND METHODS A meta-analysis was conducted according to the PRISMA guidelines. Articles published from 1980 through January 2013 were electronically and manually searched in MEDLINE (Ovid), the Cochrane Register of Controlled Trials, the Database of Abstracts of Effects, and the Cochrane Database of Systematic Reviews. Clinical reports on single intervention sinus augmentation with root-form implants, a minimum of 10 patients and 6 months of loading were eligible for inclusion if implant survival was stated or calculable. Results were calculated by non-parametric univariate Kaplan-Meier analysis and Bayesian multivariate interval-censored Cox regression. RESULTS A total of 122 publications on 16268 endosseous implants placed in grafted maxillary sinus were included. The treatment parameters surgical approach, grafting material and implant type showed no selective preference. However, application of membranes showed a significantly reduced hazard-ratio, independent of other co-factors. CONCLUSIONS The use of membranes is the most significant factor to achieve long-term implant survival in sinus augmentation procedures. More data exceeding 3 years follow-up are needed to address prospective confounding and improve clinical evidence.
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Affiliation(s)
- Fabian Duttenhoefer
- Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg, Freiburg, Germany
- * E-mail:
| | - Cyriel Souren
- Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg, Freiburg, Germany
| | | | - Dominik Emmerich
- Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg, Freiburg, Germany
| | - Ralf Schön
- Department of Oral and Craniomaxillofacial Surgery, Malteser Krankenhaus St. Josefshospital, Krefeld-Uerdingen, Germany
| | - Sebastian Sauerbier
- Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg, Freiburg, Germany
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Influence of Acidic Fibroblast Growth Factor on Bone Regeneration in Experimental Cranial Defects Using Spongostan and Bio-Oss as Protein Carriers. J Craniofac Surg 2013; 24:1507-14. [DOI: 10.1097/scs.0b013e31828f2469] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022] Open
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Schliephake H. Clinical Efficacy of Growth Factors to Enhance Tissue Repair in Oral and Maxillofacial Reconstruction: A Systematic Review. Clin Implant Dent Relat Res 2013; 17:247-73. [DOI: 10.1111/cid.12114] [Citation(s) in RCA: 52] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
Affiliation(s)
- Henning Schliephake
- Abteilung für Mund-, Kiefer-und Gesichtschirurgie; Georg-August-Universität; Göttingen Germany
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Suzuki S, Morimoto N, Ikada Y. Gelatin gel as a carrier of platelet-derived growth factors. J Biomater Appl 2013; 28:595-606. [DOI: 10.1177/0885328212468183] [Citation(s) in RCA: 20] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
Currently, patient’s own growth factors from platelet-rich plasma and platelet-rich fibrin have been clinically used for repair and regeneration of defective tissues. In platelet-rich plasma and platelet-rich fibrin, fibrin gel is formed from blood fibrinogen and functions as a carrier for growth factors. In this study, the growth factors were extracted from the platelet-rich fibrin and incorporated into a gelatin gel by mixing the platelet-rich fibrin extract and gelatin solution before cross-linking with glutaraldehyde. About 70% of TGF-β1 was found to be released in vitro from the gelatin gel containing the platelet-rich fibrin extract into phosphate-buffered saline (–) in 7 days. The gelatin gel containing basic fibroblast growth factor, prepared as a comparison, showed a similar release profile. The gelatin gels were slowly degraded with time after subcutaneous implantation on the back of rats, and the gel containing the platelet-rich fibrin extract strongly induced neovascularization and granulation tissue formation around the implantation site compared to the gel only and the gel containing basic fibroblast growth factor, platelet-rich plasma, or platelet-rich fibrin. The gelatin gel containing the platelet-rich fibrin extract was attempted as wound dressing on a full-thickness skin defect model. After 2 weeks of application, the gel was found to be more effective in acceleration of wound healing than the commonly used platelet-rich plasma.
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Affiliation(s)
- Shuko Suzuki
- Biomedical Engineering, Graduate School of Medicine, Nara Medical University, Japan
- Queensland Eye Institute, Australia
| | - Natsumi Morimoto
- Biomedical Engineering, Graduate School of Medicine, Nara Medical University, Japan
| | - Yoshito Ikada
- Biomedical Engineering, Graduate School of Medicine, Nara Medical University, Japan
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Antonello GDM, Torres do Couto R, Giongo CC, Corrêa MB, Chagas Júnior OL, Lemes CHJ. Evaluation of the effects of the use of platelet-rich plasma (PRP) on alveolar bone repair following extraction of impacted third molars: prospective study. J Craniomaxillofac Surg 2013; 41:e70-5. [PMID: 23352081 DOI: 10.1016/j.jcms.2012.11.003] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/02/2012] [Revised: 11/05/2012] [Accepted: 11/05/2012] [Indexed: 01/26/2023] Open
Abstract
Platelet-rich plasma (PRP) is rich in growth factors, target-specific polypeptides that play a role in cell proliferation and differentiation and can thus encourage wound repair. This study sought to assess the effects of PRP on new bone formation in a sample of 25 patients with clinical indications for extraction of all four impacted third molars with similar orientation, depth, and root morphology. Immediately after extraction, sockets on one side received PRP, whereas those on the other side (control) did not. Patients underwent 6 months of clinical and radiographic follow-up. Periapical radiographs were scanned and bone repair was assessed by image histogram analysis. The radiographic densities of the extraction sockets were compared to the densities of the distal surfaces of the adjacent teeth, with the difference between both serving to distinguish the PRP and control sides. Results showed that healing of PRP-treated sockets was significantly different from that of control sockets at 1-, 3-, and 6-month follow-up, in mandibular and maxillary sockets alike. The results of this sample suggest that PRP provides a safe and effective means of speeding alveolar bone repair.
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Affiliation(s)
- Guilherme de Marco Antonello
- Faculdade de Odontologia, Universidade Federal de Pelotas, Rua Gomes Carneiro, 1, Centro, CEP 96010-610, Pelotas, RS, Brazil.
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Sollazzo V, Palmieri A, Scapoli L, Martinelli M, Girardi A, Alviano F, Pellati A, Perrotti V, Carinci F. Bio-Oss®acts on Stem cells derived from Peripheral Blood. Oman Med J 2012; 25:26-31. [PMID: 22125694 DOI: 10.5001/omj.2010.7] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/29/2009] [Accepted: 11/02/2009] [Indexed: 11/03/2022] Open
Abstract
OBJECTIVES This study aims to study how Bio-Oss® can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells markers using real time Reverse Transcription-Polymerase Chain Reaction. METHODS PB-hMSCs stem preparations were obtained for gradient centrifugation from peripheral blood of healthy anonymous volunteers, using the Acuspin System-Histopaque 1077. The samples were then cultured for 7 days for RNA processing, and the expression was quantified using real time PCR. RESULTS Bio-Oss® caused an induction of osteoblast transcriptional factor like RUNX2 and of bone related genes; SPP1 and FOSL1. In contrast, the expression of ENG was significantly decreased in stem cells treated with Bio-Oss® with respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells. CONCLUSION The results obtained can be relevant to enhance the understanding of the molecular mechanism of bone regeneration and can act as a model for comparing other materials with similar clinical effects.
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Parizi AM, Oryan A, Shafiei-Sarvestani Z, Bigham AS. Human platelet rich plasma plus Persian Gulf coral effects on experimental bone healing in rabbit model: radiological, histological, macroscopical and biomechanical evaluation. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE 2012; 23:473-483. [PMID: 22057970 DOI: 10.1007/s10856-011-4478-1] [Citation(s) in RCA: 40] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/27/2011] [Accepted: 10/28/2011] [Indexed: 05/31/2023]
Abstract
Coral is an osteoconductive material used as a bone graft extender and human platelet rich plasma has been used as a source of osteoinductive factor. A combination of human platelet rich plasma and coral is expected to create a composite with both osteoconductive and osteoinductive properties. This study examined the effect of a combination of human platelet rich plasma and coral on osteogenesis in vivo using rabbit model of bone healing. A critical size defect of 10 mm elongation was created in the radial diaphysis of 36 rabbit and either supplied with coral-human PRP, or coral alone or left empty (control group). The platelets in the PRP were about 10.1 fold compared to normal blood. Radiographs of each forelimb was taken postoperatively on 1st day and then at the 2nd, 4th, 6th and 8th weeks post injury to evaluate bone formation, union and remodeling of the defect. The operated radiuses were removed on 56th postoperative day and were grossly and histopathologically evaluated. In addition, biomechanical test was conducted on the operated and normal forearms of the rabbits. This study demonstrated that coral-human PRP (hPRP), could promote bone regeneration in critical size defects with a high regenerative capacity. The results of the present study demonstrated that coral-hPRP could be an attractive alternative for reconstruction of the major diaphyseal defects of the long bones in animal models.
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Affiliation(s)
- A Meimandi Parizi
- Department of Veterinary Surgery and Radiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
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Oryan A, Meimandi Parizi A, Shafiei-Sarvestani Z, Bigham AS. Effects of combined hydroxyapatite and human platelet rich plasma on bone healing in rabbit model: radiological, macroscopical, hidtopathological and biomechanical evaluation. Cell Tissue Bank 2011; 13:639-51. [PMID: 22180011 DOI: 10.1007/s10561-011-9285-x] [Citation(s) in RCA: 57] [Impact Index Per Article: 4.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/07/2011] [Accepted: 12/12/2011] [Indexed: 11/29/2022]
Abstract
Hydroxyapatite is an osteoconductive material used as a bone graft extender and exhibits excellent biocompatibility with soft tissues such as skin, muscle and gums, making it an ideal candidate for orthopedic and dental implants or components of implants. Synthetic hydroxyapatite has been widely used in repair of hard tissues, and common uses include bone repair, bone augmentation, as well as coating of implants or acting as fillers in bone or teeth. On the other hand, human platelet rich plasma (hPRP) has been used as a source of osteoinductive factor. A combination of hPRP and hydroxyapatite is expected to create a composite with both osteoconductive and osteoinductive properties. This study examined the effect of a combination of hydroxyapatite and hPRP on osteogenesis in vivo, using rabbit model bone healing. A critical size defect of 10 mm long was created in the radial diaphysis of 36 rabbit and either supplied with hydroxyapatite-human PRP or hydroxyapatite or was left empty (control group). Radiographs of each forelimb were taken postoperatively on 1st day and then at the 2nd, 4th, 6th and 8th weeks post injury to evaluate bone formation, union and remodeling of the defect. The operated radiuses of half of the animals in each group were removed on 56th postoperative day and were grossly and histopathologically evaluated. In addition, biomechanical test was conducted on the operated and normal forearms of the other half of the animals of each group. This study demonstrated that hydroxyapatite-humanPRP, could promote bone regeneration in critical size defects with a high regenerative capacity. The results of the present study demonstrated that hydroxyapatite-hPRP could be an attractive alternative for reconstruction of the major diaphyseal defects of the long bones in animal models.
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Affiliation(s)
- A Oryan
- Department of Veterinary Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
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Tuna T, Yorgidis M, Strub JR. Prognosis of implants and fixed restorations after lateral sinus elevation: a literature review. J Oral Rehabil 2011; 39:226-38. [PMID: 21972928 DOI: 10.1111/j.1365-2842.2011.02259.x] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
Abstract
Aim of this review was to investigate the prognosis of implants inserted in augmented sinuses and fixed restorations supported by these implants. Special attention was given to the impact of grafting material, time of implant placement, residual bone height and type of fixed restoration. An electronic search in PubMed, the German database medpilot and the Cochrane Library was executed followed by supplementary manual search in relevant journals. The search was limited to human studies published up to November 2010. Only publications in English and German, in peer-reviewed journals, were considered. After the initial search and application of selection criteria on titles and abstracts, a full-text analysis of 67 articles was performed, out of which six prospective and three retrospective studies were finally included in the review. The heterogeneous properties of the identified articles did not allow systematic analysis of the data. Success rates of implants were between 96·3% and 100%, survival rates were between 75% and 100%, and survival rates of single crowns, splinted crowns and fixed partial dentures ranged between 96·4% and 100% after a follow-up of 12-101 months. Within the limits of this review, the prognosis of implants and fixed restorations seemed not to be influenced by the type of restorations, graft material, residual bone height and time of implant placement. However, conclusions of this review are based on studies with low level of evidence; therefore, careful interpretation is required. Multicentre randomised controlled clinical trials with sufficient statistical power concentrating on few factors are needed to reach sound conclusions.
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Affiliation(s)
- T Tuna
- Department of Prosthodontics, Albert-Ludwigs-University, Freiburg School of Dentistry, Albert-Ludwigs-University, Freiburg, Germany.
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Poeschl PW, Ziya-Ghazvini F, Schicho K, Buchta C, Moser D, Seemann R, Ewers R, Schopper C. Application of platelet-rich plasma for enhanced bone regeneration in grafted sinus. J Oral Maxillofac Surg 2011; 70:657-64. [PMID: 21840102 DOI: 10.1016/j.joms.2011.04.027] [Citation(s) in RCA: 33] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/20/2010] [Revised: 04/01/2011] [Accepted: 04/30/2011] [Indexed: 10/17/2022]
Abstract
PURPOSE The present study was conducted to evaluate the effect of platelet-rich plasma (PRP) on new bone formation and remodeling after grafting of the maxillary sinus with an algae-derived hydroxyapatite AlgOss/C Graft/Algipore. MATERIALS AND METHODS Fourteen consecutive patients with severely atrophic maxillae underwent uni- or bilateral grafting of the maxillary sinus with a mixture of collected bone, algae-derived hydroxyapatite AlgOss/C Graft/Algipore (ratio 1:10), and a combined addition of PRP and thrombin (Tissucol Kit; Baxter, Vienna, Austria) to allow for fast clotting. After an average healing period of 7.1 months bone samples were retrieved. Patients from a former consecutive series treated without PRP served as control group. Statistical analysis was done by Welch 2-sample t test and mixed linear model testing. RESULTS In the coronal specimen portions, mean values for newly formed bone area, biomaterial area and marrow space of 32.2% ± 10.4%, 20.1% ± 13.0%, and 47.7% ± 8.5% were found with PRP, respectively. In the control group the corresponding values were 27.6% ± 13.4%, 20.3% ± 12.9%, and 52.1% ± 9.3%. In the apical specimen portions in the PRP group, the newly formed bone area, biomaterial area, and marrow space was 25.7% ± 15.0%, 23.4% ± 14.9%, and 50.9% ± 12.5%, respectively. The corresponding values in the control group were 17.0% ± 8.6%, 34.5% ± 11.2%, and 48.5% ± 8.5%. CONCLUSIONS Statistical evaluation of the samples proved significantly better overall resorption of algae-derived hydroxyapatite AlgOss/C Graft/Algipore and increased new bone formation when PRP was used, especially in the apical region.
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Affiliation(s)
- Paul W Poeschl
- Department of Craniomaxillofacial and Oral Surgery, Medical University of Vienna, Vienna, Austria
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Avila-Ortiz G, Neiva R, Galindo-Moreno P, Rudek I, Benavides E, Wang HL. Analysis of the influence of residual alveolar bone height on sinus augmentation outcomes. Clin Oral Implants Res 2011; 23:1082-8. [DOI: 10.1111/j.1600-0501.2011.02270.x] [Citation(s) in RCA: 30] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 05/31/2011] [Indexed: 12/25/2022]
Affiliation(s)
- Gustavo Avila-Ortiz
- Department of Periodontics and Oral Medicine; University of Michigan School of Dentistry; Ann Arbor; MI; USA
| | - Rodrigo Neiva
- Department of Periodontology; University of Florida College of Dentistry; Gainesville; FL; USA
| | | | - Ivan Rudek
- Department of Periodontics and Oral Medicine; University of Michigan School of Dentistry; Ann Arbor; MI; USA
| | - Erika Benavides
- Department of Periodontics and Oral Medicine; University of Michigan School of Dentistry; Ann Arbor; MI; USA
| | - Hom-Lay Wang
- Department of Periodontics and Oral Medicine; University of Michigan School of Dentistry; Ann Arbor; MI; USA
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Cabbar F, Güler N, Kürkcü M, Işeri U, Sençift K. The effect of bovine bone graft with or without platelet-rich plasma on maxillary sinus floor augmentation. J Oral Maxillofac Surg 2011; 69:2537-47. [PMID: 21798642 DOI: 10.1016/j.joms.2011.03.040] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2010] [Revised: 03/15/2011] [Accepted: 03/30/2011] [Indexed: 01/09/2023]
Abstract
PURPOSE The purpose of this study was to compare Unilab Surgibone (USB) (Mississauga, Ontario, Canada), a bone xenograft (bovine), with platelet-rich plasma (PRP) and USB without PRP to augment the human maxillary sinus in preparation to receive dental implants. PATIENTS AND METHODS Patients who had bilateral pneumatized maxillary sinuses were included. Sinuses on one side were augmented with the USB-PRP combination and formed the study group, whereas the opposite-side sinuses were augmented with USB alone and served as controls. Bone biopsy specimens were taken during implant placement at 6.8 ± 0.9 months after maxillary sinus floor augmentation. Resonance frequency analysis measurements were performed at implant placement (first control) and before the prosthetic stage (second control), at 6.5 ± 0.7 months after implant surgery. RESULTS There were 10 patients (7 men and 3 women; mean age, 53.7 ± 0.8 years). Integration between new bone and residual grafts was histologically observed in all samples. The volumes of soft tissue were 59.9% ± 7.5% and 57.8% ± 4.4% in the control and study groups, respectively; residual graft, 21.9% ± 6.6% and 23.6% ± 5.9%, respectively; new bone, 15.8% ± 4.8% and 16.0% ± 3.8%, respectively; and trabecular bone, 64.7% ± 22.5% and 69.1% ± 18.6%, respectively. A positive correlation was found between new bone volume and trabecular bone volume (P = .0001). The mean Implant Stability Quotient (ISQ) values were 71.7 ± 4.9 and 70.3 ± 5.7 in the control and study groups, respectively, at first control and 75.4 ± 6.4 and 74.4 ± 6.4, respectively, at second control. The mean ISQ values at second control in both groups were significantly higher than at first control (P = .043 and P = .028, respectively). No statistically significant differences were observed between groups (P > .05). CONCLUSION The combination of USB and PRP does not have any effect on new bone formation and implant stabilization.
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Affiliation(s)
- Fatih Cabbar
- Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Yeditepe University, Istanbul, Turkey
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Bassil J, Senni K, Changotade S, Baroukh B, Kassis C, Naaman N, Godeau G. Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human. J Periodontal Res 2011; 46:756-62. [PMID: 21793827 DOI: 10.1111/j.1600-0765.2011.01400.x] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
Abstract
BACKGROUND AND OBJECTIVE The aim of the present study was to analyse the expression of MMP-2, MMP-9 and MMP-13 in newly formed bone following maxillary sinus augmentation using inorganic bovine bone substitute, because these MMPs play a major role in bone remodeling and bone resorption. MATERIAL AND METHODS Deproteinized bovine bone (Bio-Oss(®)) was used to fill cavities after elevating the sinus mucosa. Twenty patients with edentulous posterior maxilla were treated with 20 sinus-augmentation procedures using a two-stage technique. Forty-nine Straumann(®) endosseous implants were used to complete the implant-prosthetic rehabilitation. One cylinder-shaped bone biopsy from each patient was taken from the augmented maxillary region using trephine burs at the second stage of surgery, 8 months after grafting. A biopsy was also taken as a control from the upper molar region from six different patients who did not undergo the sinus procedure. All biopsies were subjected to biochemical analysis and staining for TRAP. RESULTS No implant losses or failures occurred. The large number of TRAP-positive multinucleated osteoclasts in resorption lacunae indicated that the resorption was very active in all grafts, in contrast with the control group. Zymography and western blot analysis demonstrated a significantly increased expression of MMP-2, MMP-9 and MMP-13 in the newly formed bone compared with controls (p < 0.05). CONCLUSION The quantity of osteoclastic cells and the increased expression of proteolytic enzymes suggest that 8 months after grafting, inorganic bovine bone is slowly resorbing and is the site of important remodeling of the newly formed bone by means of resorption and synthesis.
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Affiliation(s)
- J Bassil
- Faculty of Dental Medicine, St Joseph University, Beirut, Lebanon.
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Célio-Mariano R, de Melo WM, Carneiro-Avelino C. Comparative radiographic evaluation of alveolar bone healing associated with autologous platelet-rich plasma after impacted mandibular third molar surgery. J Oral Maxillofac Surg 2011; 70:19-24. [PMID: 21778014 DOI: 10.1016/j.joms.2011.03.028] [Citation(s) in RCA: 52] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/13/2005] [Revised: 03/28/2011] [Accepted: 03/28/2011] [Indexed: 11/30/2022]
Abstract
PURPOSE This study radiographically evaluated the performance of autologous platelet-rich plasma (PRP) applied in tooth sockets. PATIENTS AND METHODS Thirty extractions of bilateral impacted mandibular third molars were performed in 15 volunteers (7 men, 8 women; 18 to 22 years old). After extraction of right and left mandibular third molars, the socket at 1 side received the autologous PRP (PRP group) and the other was filled with blood clot (control group). Millimeter periapical radiographs were obtained 7 days, 1 month, and 2, 3, and 6 months postoperatively. Radiographic bone density was quantified 3 times by the same examiner at different moments using HLImage 97 software, and data were statistically analyzed by Statgraf 7.0 software (analysis of variance and Tukey test). RESULTS In general, there was significantly faster bone formation in sockets treated with PRP (P < .01). Significant bone formation was observed in the first month (P < .01), second month (P < .05), and third month (P < .01) for the PRP group. No statistical differences were observed on the seventh day and sixth month of investigation, yet there were higher means of radiographic bone density in sockets treated with PRP. In the control group, men exhibited significant bone repair compared with women (P < .05). CONCLUSION Autologous PRP was found to accelerate alveolar bone regeneration, and men presented better repair after tooth extraction.
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Affiliation(s)
- Ronaldo Célio-Mariano
- Department of Clinic and Surgery, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
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Gau CH, Shen EC, Tu HP, Chiu HC, Fu E, Wang WN, Chiang CY. Freezing procedure without thrombin activation to retain and store growth factors from platelet concentrates. J Dent Sci 2011. [DOI: 10.1016/j.jds.2011.03.007] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/28/2022] Open
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Del Fabbro M, Bortolin M, Taschieri S, Weinstein RL. Effect of autologous growth factors in maxillary sinus augmentation: a systematic review. Clin Implant Dent Relat Res 2011; 15:205-16. [PMID: 21453395 DOI: 10.1111/j.1708-8208.2011.00343.x] [Citation(s) in RCA: 44] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/28/2022]
Abstract
PURPOSE The aim of the present study was to systematically evaluate the effect of autogenous platelet concentrates on the clinical and histomorphometric outcomes of maxillary sinus augmentation. MATERIALS AND METHODS MEDLINE, EMBASE, and Cochrane Central Register of Controlled Trials were searched using a combination of specific search terms. Furthermore, a hand searching of the relevant journals and of the bibliographies of reviews was performed. Prospective comparative clinical studies were included. Implant survival and histomorphometric outcomes were evaluated. RESULTS Twelve studies were included. Four hundred forty-five sinus floor augmentation procedures were considered. No difference in implant survival was reported between test and control groups. Six studies reported a beneficial effect of platelet concentrates based on histomorphometric outcomes, while another six studies found no significant effect. A large heterogeneity was found regarding study design, surgical techniques, graft materials, clinical and histomorphometric outcome variables, and methods for preparing platelet concentrates. Favorable effects on soft tissue healing and postoperative discomfort reduction were often reported but not quantified. CONCLUSIONS A clear advantage of platelet concentrates could not be evidenced. Standardization in the experimental design is needed in order to detect the true effect of platelet concentrates in maxillary sinus augmentation procedure, especially regarding postoperative quality of life.
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Affiliation(s)
- Massimo Del Fabbro
- IRCCS Galeazzi Orthopaedic Institute, Dental Clinic, Centro di Ricerca per la Salute Orale, Department of Health Technologies, University of Milan, Milan, Italy.
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Pikdöken L, Gürbüzer B, Küçükodacı Z, Urhan M, Barış E, Tezulaş E. Scintigraphic, Histologic, and Histomorphometric Analyses of Bovine Bone Mineral and Autogenous Bone Mixture in Sinus Floor Augmentation: A Randomized Controlled Trial—Results After 4 Months of Healing. J Oral Maxillofac Surg 2011; 69:160-9. [DOI: 10.1016/j.joms.2010.07.036] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/15/2010] [Revised: 07/14/2010] [Accepted: 07/23/2010] [Indexed: 11/17/2022]
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Garcia RV, Gabrielli MAC, Hochuli-Vieira E, Spolidorio LC, Filho JGP, Neto FAD, de Cardoso LAG, Shibli JA. Effect of platelet-rich plasma on peri-implant bone repair: a histologic study in dogs. J ORAL IMPLANTOL 2010; 36:281-90. [PMID: 20735264 DOI: 10.1563/aaid-joi-d-09-00056] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
The present study evaluated the effect of platelet-rich plasma (PRP) on peri-implant bone healing. A total of 9 mongrel dogs received 36 dental implants with sandblasted acid-etched surface in lower jaws in a split-mouth design: in the PRP group (n=18 implants) the implants were placed in association with PRP, and in the control group (n=18 implants) the implants were placed without PRP. Biopsies were obtained and prepared for histologic and histometric analysis after 15, 30, and 55 days of healing. The biopsies retrieved at 15 days showed delicate bone trabeculae formed by immature bone with presence of numerous osteoblasts for both groups. At 30 days the trabeculae presented reversal lines and evident lamellar disposition, where some thread spaces were filled by bone and dense connective tissue. At 55 days, bone healing was not altered in the control group, and histologic aspects were variable for the group treated with PRP. There was no significant difference between the groups for bone-to-implant contact (P>.05). PRP did not enhance bone formation around sandblasted acid-etched implants.
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Affiliation(s)
- Ricardo V Garcia
- Division of Periodontology, Dental School at Araraquara, São Paulo State University, São Paulo, Brazil.
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Platelet-rich plasma in sinus augmentation procedures: a systematic literature review: Part II. IMPLANT DENT 2010; 19:145-57. [PMID: 20386218 DOI: 10.1097/id.0b013e3181cd706d] [Citation(s) in RCA: 45] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
Abstract
BACKGROUND Although platelet-rich plasma (PRP) has been extensively studied for over a decade, there are no definitive reports, which prove the benefit of using PRP in sinus augmentation procedures. In addition, no systematic literature review has been done to report the benefit of treatment outcome in patients who received PRP in conjunction with bone/bone substitutes in maxillary sinus augmentation procedures. Therefore, it can be rightly stated that evidence for an adjunctive benefit of using PRP with bone grafts in sinus augmentation procedures is equivocal and inconclusive. AIM : The objective of this systematic literature review was to examine this literature in determining whether PRP with bone and bone substitutes leads to more rapid and effective bone regeneration clinically, radiographically, and histologically with sinus augmentation procedures and was there any clinical data parallel to animal experiments providing clinical evidence in sinus augmentation procedures? METHODS A systematic review of randomized clinical trials of at least 6 months duration was conducted comparing PRP and bone/bone substitutes (test group) to bone/bone substitutes (control group) alone. Electronic databases such as MEDLINE and CENTRAL (Cochrane central register of controlled clinical trials) were searched for relevant articles. The reference list of all included articles was searched along with unpublished clinical trials whose abstracts were available. RESULTS Although, there is a lack of human studies, which show benefit of using PRP in conjunction with bone grafting materials, it can be stated that use of PRP does lead to early regeneration and reduction in healing time of soft and hard tissues. However, no significant statistical or clinical benefit was reported from studies that would satisfy the inclusion criteria. This study answers the question very clearly that at this point of time, there is no human study that strongly supports the benefit of using PRP in sinus augmentation procedures. CONCLUSION There is a paucity of clinically controlled trials regarding benefits of PRP in sinus augmentation procedures. Theoretically, it seems to have significant beneficial effects on the soft and hard tissue healing; however, the disparity in study design, surgical techniques, and different outcome assessment variables used, makes it difficult to assess the practical benefit of its clinical use. Although no obvious positive effects of PRP on healing of bone graft material in maxillary sinus augmentation procedures were noted, the handling of the particulate bone grafts was improved.
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Cages augmented with mineralized collagen and platelet-rich plasma as an osteoconductive/inductive combination for interbody fusion. Spine (Phila Pa 1976) 2010; 35:740-6. [PMID: 20228704 DOI: 10.1097/brs.0b013e3181bdc6cc] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/01/2023]
Abstract
STUDY DESIGN After anterior cervical discectomy, fusion was radiologically, biomechanically, and histologically assessed in a sheep spine fusion model. OBJECTIVE To evaluate the efficacy of a platelet-rich plasma (PRP) application combined with a mineralized collagen matrix (MCM) as an alternative to autologous cancellous iliac crest bone grafts in a spine fusion model. SUMMARY OF BACKGROUND DATA PRP has the ability to stimulate bone and tissue healing. MCM is a recently developed osteoconductive material. Up to now, no comparative evaluation of PRP in combination with a MCM at the cervical spine has been performed in vivo. METHODS Twenty-four sheep (N = 8/group) underwent C3/4 discectomy and fusion: group 1, titanium cage filled with autologous cancellous iliac crest bone graft; group 2, titanium cage filled with MCM; and group 3, titanium cage filled with MCM and PRP. Radiographic evaluation was performed before surgery and after 1, 2, 4, 8, and 12 weeks, respectively. After 12 weeks, fusion sites were evaluated using functional radiographic views and quantitative computed tomographic scans to assess bone mineral density. Furthermore, histomorphologic and histomorphometrical analyses were performed to evaluate fusion. RESULTS In comparison with the titanium cage group filled with autologous cancellous iliac crest bone grafts representing the control group, MCM-alone group showed a slightly lower fusion rate in the radiographic and the histomorphometrical analysis. The addition of PRP could not enhance this finding. There was no significant difference between MCM and MCM + PRP group in radiologic and histologic findings. CONCLUSION The MCM alone is not able to replace autologous bone grafts. Early activation of the platelets by calcium, which is released from mineralized collagen, could be the reason for the insufficient osteoinductive effect of PRP. In consequence, the combined application of mineralized collagen and PRP had no significant osteoinductive effect in this model.
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Amrani S, Anastassov GE, Montazem AH. Mandibular ramus/coronoid process grafts in maxillofacial reconstructive surgery. J Oral Maxillofac Surg 2010; 68:641-6. [PMID: 20171483 DOI: 10.1016/j.joms.2009.09.100] [Citation(s) in RCA: 20] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/23/2009] [Revised: 08/26/2009] [Accepted: 09/23/2009] [Indexed: 01/24/2023]
Abstract
PURPOSE To evaluate the utility of autogenous extended mandibular ramus and coronoid process bone grafts for maxillofacial reconstructive surgery. PATIENTS AND METHODS Twelve patients aged 23 to 76 years (mean, 52) who underwent extended ramus/coronoid process grafts for reconstruction of maxillofacial deformities due to trauma, alveolar atrophy, or iatrogenic nasal deformity. All patients had either unilateral or bilateral combined coronoid process-mandibular ramus bone grafts for their reconstruction. There was 1 nasal reconstruction, 2 unilateral mandibles, 1 bilateral mandible, 4 unilateral maxillas, 1 unilateral maxilla and mandible combined, and 1 bilateral maxilla and mandible combined. RESULTS The procedure was considered a success when the patient's deformities were reconstructed ad integrum and when there were no failures of the dental implants placed in the augmented areas as of the longest follow-up. All patients were successfully reconstructed. There was 1 infection at a donor site that resolved with local care and oral antibiotics. All but 1 of the maxillary and mandibular alveolar augmentations underwent endosteal implant placement approximately 4 to 6 months following grafting. The nasal reconstruction restored normal function and symmetry. CONCLUSION Using both the coronoid process of the mandible and the mandibular ramus as a source for autogenous bone graft can provide sufficient bone in quantity and quality for selected maxillofacial reconstructions.
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Affiliation(s)
- Saar Amrani
- Department of Oral and Maxillofacial Surgery, Mount Sinai School of Medicine, New York, NY, USA
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Garg AK. Immediate Loading of Implants in the Edentulous Patient. IMPLANT DENT 2010. [DOI: 10.1016/b978-0-323-05566-6.00015-0] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/27/2022]
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Song JY, Kweon H, Kwon KJ, Park YW, Kim SG. The bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) in the calvaria defect of rabbit. J Korean Assoc Oral Maxillofac Surg 2010. [DOI: 10.5125/jkaoms.2010.36.4.250] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022] Open
Affiliation(s)
- Ji-Young Song
- Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea
| | - HaeYong Kweon
- National Academy of Agricultural Science, RDA, Suwon, Korea
| | - Kwang Jun Kwon
- Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea
| | - Young-Wook Park
- Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea
| | - Seong-Gon Kim
- Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea
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Lee CYS, Rohrer MD, Prasad HS, Stover JD, Suzuki JB. Sinus grafting with a natural fluorohydroxyapatite for immediate load: a study with histologic analysis and histomorphometry. J ORAL IMPLANTOL 2009; 35:164-75. [PMID: 19813420 DOI: 10.1563/1548-1336-35.4.164] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/04/2023]
Abstract
The goal of this retrospective study was to evaluate the survival rates of dental implants placed in sinuses grafted with a 50:50 composite ratio of autogenous bone and a natural flourohydroxyapatite (FHA) combined with platelet-rich plasma (PRP) using an immediate-load protocol. The authors hypothesized that a 50:50 composite ratio of FHA and autogenous bone combined with PRP would permit immediate loading without compromising implant survival rates. Eleven patients with bilateral partial edentulism of the posterior maxilla were enrolled in this retrospective study. Autogenous bone used in the graft procedure was harvested from the tibia of the left lower extremity. Each patient was grafted with a 50:50 composite ratio of autogenous bone and FHA. Membranes were not used to cover the lateral wall osteotomy site. Platelet-rich plasma was added to the graft material to accelerate and enhance bone regeneration. Four to 6 months after the grafting procedure, 37 hydroxyapatite-coated dental implants were surgically placed and immediately loaded between 72 hours and 5 days later with custom titanium abutments and acrylic provisional restorations placed out of functional occlusion. Six months later, definitive ceramometal restorations were cemented on to the custom abutments. Patients were observed over a 52-week period. The overall implant survival rate was 97.3%. Histologic and histomorphometric analysis of core samples revealed formation of new vital bone in different graft specimens ranging from 23% to 34%. In each core bone sample, 100% of the bone sample was determined to be vital. In the grafted maxillary sinus, the natural FHA combined with autogenous bone in a 50:50 composite ratio with PRP is a suitable graft material permitting immediate load without compromising implant survival rates while decreasing the overall healing time.
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