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Paiwand S, Schäfer S, Kopp A, Beikler T, Fiedler I, Gosau M, Fuest S, Smeets R. Antibacterial potential of silver and zinc loaded plasma-electrolytic oxidation coatings for dental titanium implants. Int J Implant Dent 2025; 11:12. [PMID: 39960576 PMCID: PMC11833008 DOI: 10.1186/s40729-025-00595-w] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/28/2024] [Accepted: 01/09/2025] [Indexed: 02/20/2025] Open
Abstract
Peri-implantitis is known as an inflammatory condition affecting the soft and hard tissue around dental implants. A promising strategy to prevent these conditions is the use of antibacterial implants. This study aimed to evaluate the antibacterial potential of titanium (Ti) dental implants modified using plasma-electrolytic oxidation (PEO). The modified surfaces were subsequently loaded with silver (Ag) (n = 6) and zinc (Zn) (n = 6) ions and compared to unloaded Ti specimens (n = 6), with untreated specimens serving as controls. The specimens (each n = 5) were incubated in a culture medium containing a mixture of specific anaerobic bacterial strains. Scanning electron microscopy (SEM) was used to visualize the bacterial biofilm on each specimen. In addition, total bacterial deoxxyribonucleic acid (DNA) and the number of viable bacteria were determined using quantitative real-time polymerase chain reaction (qrt-PCR) and colony forming unit analysis (CFU), respectively. The results of the CFU analysis showed a 2 log (99%) reduction in viable bacteria in the samples loaded with Ag and Zn compared to the unloaded control group (p < 0.05). Moreover, significantly lower bacterial DNA counts were detected with a 5 log reduction (99.999%) in the Ag and Zn samples compared to the positive control group (bacterial mixed culture solution, p < 0.05). Therefore, it was considered that Ag and Zn loaded Ti implants may be a promising addition to current approaches to enable advanced antibacterial dental implants. However, further studies should be conducted to evaluate the in vivo cytocompatibility of the developed specimens.
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Affiliation(s)
- Sabawun Paiwand
- Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
- Department of Neurosurgery, University Medical Center Schleswig-Holstein, Campus Kiel, Arnold-Heller-Strasse 3, 24105, Kiel, Germany
| | - Sogand Schäfer
- Department of Oral and Maxillofacial Surgery, Devision of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
- Division of Plastic and Reconstructive Surgery, Department of Surgery, Children's Hospital of Philadelphia, 3401 Civic Center Blvd., Philadelphia, PA, 19104, USA
| | | | - Thomas Beikler
- Department of Periodontics, Preventive and Restorative Dentistry, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
| | - Imke Fiedler
- Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
| | - Martin Gosau
- Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
| | - Sandra Fuest
- Department of Oral and Maxillofacial Surgery, Devision of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
| | - Ralf Smeets
- Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany.
- Department of Oral and Maxillofacial Surgery, Devision of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany.
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Liu X, Zhou C, Xie Q, Xia L, Liu L, Bao W, Lin H, Xiong X, Zhang H, Zheng Z, Zhao J, Liang W. Recent advances in layer-by-layer assembly scaffolds for co-delivery of bioactive molecules for bone regeneration: an updated review. J Transl Med 2024; 22:1001. [PMID: 39501263 PMCID: PMC11539823 DOI: 10.1186/s12967-024-05809-0] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/07/2024] [Accepted: 10/25/2024] [Indexed: 11/08/2024] Open
Abstract
Orthopedic implants have faced challenges in treating bone defects due to various factors, including inadequate osseointegration, oxidative stress, bacterial infection, immunological rejection, and poor individualized treatment. These challenges profoundly affect both the results of treatment and patients' daily lives. There is great promise for the layer-by-layer (LbL) assembly method in tissue engineering. The method primarily relies on electrostatic attraction and entails the consecutive deposition of electrolyte complexes with opposite charges onto a substrate, leading to the formation of homogeneous single layers that can be quickly deposited to produce nanolayer films. LbL has attracted considerable interest as a coating technology because of its ease of production, cost-effectiveness, and capability to apply diverse biomaterial coatings without compromising the primary bio-functional properties of the substrate materials. This review will look into the fundamentals and evolution of LbL in orthopedics, provide an analysis of the chemical strategy used to prepare bone implants with LbL and introduce the application of LbL bone implants in orthopedics over recent years. Among the many potential uses of LbL, such as the implementation of sustained-release and programmed drug delivery, which in turn promotes the osseointegration and the development of new blood vessels, as well as antibacterial, antioxidant, and other similar applications. In addition, we offer a thorough examination of cell behavior and biomaterial interaction to facilitate the advancement of next-generation LbL films for tissue engineering.
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Affiliation(s)
- Xiankun Liu
- Department of Orthopaedics, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, 355 Xinqiao Road, Dinghai District, Zhoushan, 316000, Zhejiang, People's Republic of China
| | - Chao Zhou
- Department of Orthopedics, Zhoushan Guanghua Hospital, Zhoushan, 316000, Zhejiang, China
| | - Qiong Xie
- Department of Orthopedics, Zhoushan Guanghua Hospital, Zhoushan, 316000, Zhejiang, China
| | - Linying Xia
- Medical Research Center, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Zhoushan, 316000, China
| | - Lu Liu
- Medical Research Center, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Zhoushan, 316000, China
| | - Wenwen Bao
- Medical Research Center, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Zhoushan, 316000, China
| | - Hongming Lin
- Department of Orthopedics, Zhoushan Guanghua Hospital, Zhoushan, 316000, Zhejiang, China
| | - Xiaochun Xiong
- Department of Orthopaedics, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, 355 Xinqiao Road, Dinghai District, Zhoushan, 316000, Zhejiang, People's Republic of China
| | - Hao Zhang
- Department of Orthopaedics, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, 355 Xinqiao Road, Dinghai District, Zhoushan, 316000, Zhejiang, People's Republic of China
| | - Zeping Zheng
- Department of Orthopaedics, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, 355 Xinqiao Road, Dinghai District, Zhoushan, 316000, Zhejiang, People's Republic of China
| | - Jiayi Zhao
- Department of Orthopaedics, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, 355 Xinqiao Road, Dinghai District, Zhoushan, 316000, Zhejiang, People's Republic of China.
| | - Wenqing Liang
- Department of Orthopaedics, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, 355 Xinqiao Road, Dinghai District, Zhoushan, 316000, Zhejiang, People's Republic of China.
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Yang G, Ming P, Niu S, Qin G, Liu H, Li D, Zhang A. An Investigation of the Efficient-Precise Continuous Electrochemical Grinding Process of Ti-6Al-4V. MATERIALS (BASEL, SWITZERLAND) 2024; 17:1729. [PMID: 38673087 PMCID: PMC11051527 DOI: 10.3390/ma17081729] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/13/2024] [Revised: 04/01/2024] [Accepted: 04/03/2024] [Indexed: 04/28/2024]
Abstract
Titanium alloys have many excellent characteristics, and they are widely used in aerospace, biomedicine, and precision engineering. Meanwhile, titanium alloys are difficult to machine and passivate readily. Electrochemical grinding (ECG) is an ideal technology for the efficient-precise machining of titanium alloys. In the ECG process of titanium alloys, the common approach of applying high voltage and active electrolytes to achieve high efficiency of material removal will lead to serious stray corrosion, and the time utilized for the subsequent finishing will be extended greatly. Therefore, the application of ECG in the field of high efficiency and precision machining of titanium alloys is limited. In order to address the aforementioned issues, the present study proposed an efficient-precise continuous ECG (E-P-C-ECG) process for Ti-6Al-4V applying high-pulsed voltage with an optimized duty cycle and low DC voltage in the efficient ECG stage and precise ECG stage, respectively, without changing the grinding wheel. According to the result of the passivation properties tests, the ideal electrolyte was selected. Optimization of the process parameters was implemented experimentally to improve the processing efficiency and precision of ECG of Ti-6Al-4V. Utilizing the process advantages of the proposed process, a thin-walled structure of Ti-6Al-4V was obtained with high efficiency and precision. Compared to the conventional mechanical grinding process, the compressive residual stress of the machined surface and the processing time were reduced by 90.5% and 63.3% respectively, and both the surface roughness and tool wear were obviously improved.
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Affiliation(s)
| | - Pingmei Ming
- School of Mechanical and Power Engineering, Henan Polytechnic University, Jiaozuo 454003, China
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Shaygani H, Seifi S, Shamloo A, Golizadeh M, Rahnamaee SY, Alishiri M, Ebrahimi S. Novel bilayer coating on gentamicin-loaded titanium nanotube for orthopedic implants applications. Int J Pharm 2023; 636:122764. [PMID: 36889413 DOI: 10.1016/j.ijpharm.2023.122764] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/03/2022] [Revised: 02/16/2023] [Accepted: 02/18/2023] [Indexed: 03/08/2023]
Abstract
Fabricating a multifunctional orthopedic implant which prevents post-surgery infection is highly desirable in advanced materials applications. However, designing an antimicrobial implant, which simultaneously promotes a sustained drug release and satisfactory cell proliferation, remains a challenge. The current study presents a drug-loaded surface-modified titanium nanotube (TNT) implant with different surface chemistry which was developed to investigate the effect of surface coating on drug release, antimicrobial activity, and cell proliferation. Accordingly, sodium alginate and chitosan were coated on the surface of TNT implants with different coating orders through layer-by-layer assembly. The coatings' swelling ratio and degradation rate were around 613% and 75%, respectively. The drug release results showed that surface-coatings prolonged the releasing profile for about 4 weeks. Chitosan coated TNTs demonstrated greater inhibition zone at 16.33mm compared with the other samples where no inhibition zone was observed. However, chitosan and alginate coated TNTs exhibited smaller inhibition zones at 48.56mm and 43.28mm, respectively, compared to bare TNT, which can be attributed to the coatings preventing the antibiotic burst release. Higher viability of cultured osteoblast cells was observed for chitosan-coated TNT as the top layer compared to the bare TNT at 12.18%, indicating improved bioactivity of TNT implants when the chitosan has the most contact with cells. Coupled with the cell viability assay, molecular dynamics (MD) simulations were carried out by placing collagen and fibronectin near the considered substrates. In agreement with cell viability results, MD simulations also indicated that chitosan had the highest adsorption energy approximately 60Kcal/mol. In summary, the proposed bilayer chitosan-coated drug-loaded TNT implant with chitosan and sodium alginate coating as the top and the bottom layers, respectively, can be a potential candidate for orthopedic applications in the light of its bacterial biofilm prevention, better osteoconductivity, and providing an adequate drug release profile.
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Affiliation(s)
- Hossein Shaygani
- School of Mechanical Engineering, Sharif University of Technology, Tehran, Iran; Stem Cell and Regenerative Medicine Institute, Sharif University of Technology, Tehran, Iran
| | - Saeed Seifi
- School of Mechanical Engineering, Sharif University of Technology, Tehran, Iran; Stem Cell and Regenerative Medicine Institute, Sharif University of Technology, Tehran, Iran
| | - Amir Shamloo
- School of Mechanical Engineering, Sharif University of Technology, Tehran, Iran; Stem Cell and Regenerative Medicine Institute, Sharif University of Technology, Tehran, Iran.
| | - Mortaza Golizadeh
- School of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran
| | - Seyed Yahya Rahnamaee
- Polymeric Materials Research Group (PMRG), School of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran
| | - Mojgan Alishiri
- School of Mechanical Engineering, Sharif University of Technology, Tehran, Iran
| | - Sina Ebrahimi
- School of Mechanical Engineering, Sharif University of Technology, Tehran, Iran
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Kim SK, Murugan SS, Dalavi PA, Gupta S, Anil S, Seong GH, Venkatesan J. Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration. BEILSTEIN JOURNAL OF NANOTECHNOLOGY 2022; 13:1051-1067. [PMID: 36247529 PMCID: PMC9531556 DOI: 10.3762/bjnano.13.92] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 06/03/2022] [Accepted: 09/08/2022] [Indexed: 06/16/2023]
Abstract
Biomimetic materials for better bone graft substitutes are a thrust area of research among researchers and clinicians. Autografts, allografts, and synthetic grafts are often utilized to repair and regenerate bone defects. Autografts are still considered the gold-standard method/material to treat bone-related issues with satisfactory outcomes. It is important that the material used for bone tissue repair is simultaneously osteoconductive, osteoinductive, and osteogenic. To overcome this problem, researchers have tried several ways to develop different materials using chitosan-based nanocomposites of silver, copper, gold, zinc oxide, titanium oxide, carbon nanotubes, graphene oxide, and biosilica. The combination of materials helps in the expression of ideal bone formation genes of alkaline phosphatase, bone morphogenic protein, runt-related transcription factor-2, bone sialoprotein, and osteocalcin. In vitro and in vivo studies highlight the scientific findings of antibacterial activity, tissue integration, stiffness, mechanical strength, and degradation behaviour of composite materials for tissue engineering applications.
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Affiliation(s)
- Se-Kwon Kim
- Department of Marine Science and Convergence Engineering, College of Science and Technology, Hanyang University, Gyeonggi-do 11558, Korea
| | - Sesha Subramanian Murugan
- Biomaterials Research Laboratory, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, Karnataka 575018, India
| | - Pandurang Appana Dalavi
- Biomaterials Research Laboratory, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, Karnataka 575018, India
| | - Sebanti Gupta
- Biomaterials Research Laboratory, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, Karnataka 575018, India
| | - Sukumaran Anil
- Department of Dentistry, Oral Health Institute, Hamad Medical Corporation, College of Dental Medicine, Qatar University, Doha, Qatar
| | - Gi Hun Seong
- Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 426-791, South Korea
| | - Jayachandran Venkatesan
- Biomaterials Research Laboratory, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, Karnataka 575018, India
- Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 426-791, South Korea
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Pawłowski Ł, Wawrzyniak J, Banach-Kopeć A, Cieślik BM, Jurak K, Karczewski J, Tylingo R, Siuzdak K, Zieliński A. Antibacterial properties of laser-encapsulated titanium oxide nanotubes decorated with nanosilver and covered with chitosan/Eudragit polymers. BIOMATERIALS ADVANCES 2022; 138:212950. [PMID: 35913239 DOI: 10.1016/j.bioadv.2022.212950] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/28/2022] [Revised: 04/22/2022] [Accepted: 05/23/2022] [Indexed: 06/15/2023]
Abstract
To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface. Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface. To ensure a controlled release of the bactericidal substance, the samples were additionally coated with a pH-sensitive chitosan/Eudragit 100 coating, also exhibiting bactericidal properties. The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques. The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated. The obtained composite coatings were hydrophilic, adjacent to the surface, and corrosion-resistant. An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect. The proposed modification was effective against both Gram-positive and Gram-negative bacteria.
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Affiliation(s)
- Łukasz Pawłowski
- Institute of Manufacturing and Materials Technology, Faculty of Mechanical Engineering and Ship Technology, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland.
| | - Jakub Wawrzyniak
- Center for Plasma and Laser Engineering, The Szewalski Institute of Fluid-Flow Machinery, Polish Academy of Sciences, Fiszera 14, 80-231 Gdańsk, Poland
| | - Adrianna Banach-Kopeć
- Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
| | - Bartłomiej Michał Cieślik
- Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
| | - Kacper Jurak
- Department of Electrochemistry, Corrosion and Materials Engineering, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk. Poland
| | - Jakub Karczewski
- Institute of Nanotechnology and Materials Engineering, Faculty of Applied Physics and Mathematics, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
| | - Robert Tylingo
- Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
| | - Katarzyna Siuzdak
- Center for Plasma and Laser Engineering, The Szewalski Institute of Fluid-Flow Machinery, Polish Academy of Sciences, Fiszera 14, 80-231 Gdańsk, Poland
| | - Andrzej Zieliński
- Institute of Manufacturing and Materials Technology, Faculty of Mechanical Engineering and Ship Technology, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland
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[Research progress of antibacterial modification of orthopaedic implants surface]. ZHONGGUO XIU FU CHONG JIAN WAI KE ZA ZHI = ZHONGGUO XIUFU CHONGJIAN WAIKE ZAZHI = CHINESE JOURNAL OF REPARATIVE AND RECONSTRUCTIVE SURGERY 2022; 36:511-516. [PMID: 35426294 PMCID: PMC9011072 DOI: 10.7507/1002-1892.202112109] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Subscribe] [Scholar Register] [Indexed: 01/24/2023]
Abstract
OBJECTIVE To summarize the related research progress of antibacterial modification of orthopaedic implants surface in recent years. METHODS The domestic and foreign related literature in recent years was extensively consulted, the research progress on antibacterial modification of orthopaedic implants surface was discussed from two aspects of characteristics of infection in orthopedic implants and surface anti-infection modification. RESULTS The orthopaedic implants infections are mainly related to aspects of bacterial adhesion, decreased host immunity, and surface biofilm formation. At present, the main antimicrobial coating methods of orthopaedic implants are antibacterial adhesion coating, antibiotic coating, inorganic antimicrobial coating, composite antimicrobial coating, nitric oxide coating, immunomodulation, three-dimensional printing, polymer antimicrobial coating, and "smart" coating. CONCLUSION The above-mentioned antibacterial coating methods of orthopedic implants can not only inhibit bacterial adhesion, but also solve the problems of low immunity and biofilm formation. However, its mechanism of action and modification are still controversial and require further research.
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Antimicrobial and Antibiofilm Coating of Dental Implants—Past and New Perspectives. Antibiotics (Basel) 2022; 11:antibiotics11020235. [PMID: 35203837 PMCID: PMC8868456 DOI: 10.3390/antibiotics11020235] [Citation(s) in RCA: 32] [Impact Index Per Article: 10.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/08/2022] [Revised: 01/27/2022] [Accepted: 02/08/2022] [Indexed: 02/01/2023] Open
Abstract
Regarded as one of the best solutions to replace missing teeth in the oral cavity, dental implants have been the focus of plenty of studies and research in the past few years. Antimicrobial coatings are a promising solution to control and prevent bacterial infections that compromise the success of dental implants. In the last few years, new materials that prevent biofilm adhesion to the surface of titanium implants have been reported, ranging from improved methods to already established coating surfaces. The purpose of this review is to present the developed antimicrobial and antibiofilm coatings that may have the potential to reduce bacterial infections and improve the success rate of titanium dental implants. All referred coating surfaces showed high antimicrobial properties with effectiveness in biofilm control, while maintaining implant biocompatibility. We expect that by combining the use of oligonucleotide probes as a covering material with novel peri-implant adjuvant therapies, we will be able to avoid the downsides of other covering materials (such as antibiotic resistance), prevent bacterial infections, and raise the success rate of dental implants. The existing knowledge on the optimal coating material for dental implants is limited, and further research is needed before more definitive conclusions can be drawn.
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Sánchez-Bodón J, Andrade del Olmo J, Alonso JM, Moreno-Benítez I, Vilas-Vilela JL, Pérez-Álvarez L. Bioactive Coatings on Titanium: A Review on Hydroxylation, Self-Assembled Monolayers (SAMs) and Surface Modification Strategies. Polymers (Basel) 2021; 14:165. [PMID: 35012187 PMCID: PMC8747097 DOI: 10.3390/polym14010165] [Citation(s) in RCA: 29] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/03/2021] [Revised: 12/28/2021] [Accepted: 12/29/2021] [Indexed: 12/15/2022] Open
Abstract
Titanium (Ti) and its alloys have been demonstrated over the last decades to play an important role as inert materials in the field of orthopedic and dental implants. Nevertheless, with the widespread use of Ti, implant-associated rejection issues have arisen. To overcome these problems, antibacterial properties, fast and adequate osseointegration and long-term stability are essential features. Indeed, surface modification is currently presented as a versatile strategy for developing Ti coatings with all these challenging requirements and achieve a successful performance of the implant. Numerous approaches have been investigated to obtain stable and well-organized Ti coatings that promote the tailoring of surface chemical functionalization regardless of the geometry and shape of the implant. However, among all the approaches available in the literature to functionalize the Ti surface, a promising strategy is the combination of surface pre-activation treatments typically followed by the development of intermediate anchoring layers (self-assembled monolayers, SAMs) that serve as the supporting linkage of a final active layer. Therefore, this paper aims to review the latest approaches in the biomedical area to obtain bioactive coatings onto Ti surfaces with a special focus on (i) the most employed methods for Ti surface hydroxylation, (ii) SAMs-mediated active coatings development, and (iii) the latest advances in active agent immobilization and polymeric coatings for controlled release on Ti surfaces.
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Affiliation(s)
- Julia Sánchez-Bodón
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
| | - Jon Andrade del Olmo
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
- i+Med S. Coop, Parque Tecnológico de Alava, Albert Einstein 15, Nave 15, 01510 Vitoria-Gasteiz, Spain;
| | - Jose María Alonso
- i+Med S. Coop, Parque Tecnológico de Alava, Albert Einstein 15, Nave 15, 01510 Vitoria-Gasteiz, Spain;
| | - Isabel Moreno-Benítez
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
| | - José Luis Vilas-Vilela
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
- BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain
| | - Leyre Pérez-Álvarez
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
- BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain
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Wang Y, Gan Z, Lu H, Liu Z, Shang P, Zhang J, Yin W, Chu H, Yuan R, Ye Y, Chen P, Rong M. Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium. Front Physiol 2021; 12:689807. [PMID: 35035356 PMCID: PMC8753411 DOI: 10.3389/fphys.2021.689807] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/01/2021] [Accepted: 10/22/2021] [Indexed: 01/03/2023] Open
Abstract
Nowadays, the bone osseointegration in different environments is comparable, but the mechanism is unclear. This study aimed to investigate the osseointegration of different bioactive titanium surfaces under normoxic or high-altitude hypoxic environments. Titanium implants were subjected to one of two surface treatments: (1) sanding, blasting, and acid etching to obtain a rough surface, or (2) extensive polishing to obtain a smooth surface. Changes in the morphology, proliferation, and protein expression of osteoblasts on the rough and smooth surfaces were examined, and bone formation was studied through western blotting and animal-based experiments. Our findings found that a hypoxic environment and rough titanium implant surface promoted the osteogenic differentiation of osteoblasts and activated the JAK1/STAT1/HIF-1α pathway in vitro. The animal study revealed that following implant insertion in tibia of rabbit, bone repair at high altitudes was slower than that at low altitudes (i.e., in plains) after 2weeks; however, bone formation did not differ significantly after 4weeks. The results of our study showed that: (1) The altitude hypoxia environment would affect the early osseointegration of titanium implants while titanium implants with rough surfaces can mitigate the effects of this hypoxic environment on osseointegration, (2) the mechanism may be related to the activation of JAK1/STAT1/HIF-1α pathway, and (3) our results suggest the osteogenesis of titanium implants, such as oral implants, is closely related to the oxygen environment. Clinical doctors, especially dentists, should pay attention to the influence of hypoxia on early osseointegration in patients with high altitude. For example, it is better to choose an implant system with rough implant surface in the oral cavity of patients with tooth loss at high altitude.
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Affiliation(s)
- Yarong Wang
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
| | - Zekun Gan
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
| | - Haibin Lu
- Department of Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
| | - Ziyi Liu
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
| | - Peng Shang
- College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, China
| | - Jian Zhang
- College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, China
| | - Wuwei Yin
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
| | - Hongxing Chu
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
| | | | - Yingxin Ye
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
| | - Pei Chen
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
- Pei Chen,
| | - Mingdeng Rong
- Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, China
- *Correspondence: Mingdeng Rong,
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11
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Zuo W, Yu L, Lin J, Yang Y, Fei Q. Properties improvement of titanium alloys scaffolds in bone tissue engineering: a literature review. ANNALS OF TRANSLATIONAL MEDICINE 2021; 9:1259. [PMID: 34532396 PMCID: PMC8421948 DOI: 10.21037/atm-20-8175] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 12/27/2020] [Accepted: 05/28/2021] [Indexed: 12/19/2022]
Abstract
Owing to their excellent biocompatibility and corrosion-resistant properties, titanium (Ti) (and its alloy) are essential artificial substitute biomaterials for orthopedics. However, flaws, such as weak osteogenic induction ability and higher Young's modulus, have been observed during clinical application. As a result, short- and long-term postoperative follow-up has found that several complications have occurred. For decades, scientists have exerted efforts to compensate for these deficiencies. Different modification methods have been investigated, including changing alloy contents, surface structure transformation, three-dimensional (3D) structure transformation, coating, and surface functionalization technologies. The cell-surface interaction effect and imitation of the natural 3D bone structure are the two main mechanisms of these improved methods. In recent years, significant progress has been made in materials science research methods, including thorough research of titanium alloys of different compositions, precise surface pattern control technology, controllable 3D structure construction technology, improvement of coating technologies, and novel concepts of surface functionalization. These improvements facilitate the possibility for further research in the field of bone tissue engineering. Although the underlying mechanism is still not fully understood, these studies still have some implications for clinical practice. Therefore, for the direction of further research, it is beneficial to summarize these studies according to the basal method used. This literature review aimed to classify these technologies, thereby providing beginners with a preliminary understanding of the field.
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Affiliation(s)
- Weiyang Zuo
- Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, China
| | - Lingjia Yu
- Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, China
| | - Jisheng Lin
- Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, China
| | - Yong Yang
- Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, China
| | - Qi Fei
- Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, China
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12
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Wang X, Mei L, Jiang X, Jin M, Xu Y, Li J, Li X, Meng Z, Zhu J, Wu F. Hydroxyapatite-Coated Titanium by Micro-Arc Oxidation and Steam-Hydrothermal Treatment Promotes Osseointegration. Front Bioeng Biotechnol 2021; 9:625877. [PMID: 34490219 PMCID: PMC8417371 DOI: 10.3389/fbioe.2021.625877] [Citation(s) in RCA: 9] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/04/2020] [Accepted: 05/13/2021] [Indexed: 02/04/2023] Open
Abstract
Titanium (Ti)-based alloys are widely used in tissue regeneration with advantages of improved biocompatibility, high mechanical strength, corrosion resistance, and cell attachment. To obtain bioactive bone–implant interfaces with enhanced osteogenic capacity, various methods have been developed to modify the surface physicochemical properties of bio-inert Ti and Ti alloys. Nano-structured hydroxyapatite (HA) formed by micro-arc oxidation (MAO) is a synthetic material, which could facilitate osteoconductivity, osteoinductivity, and angiogenesis on the Ti surface. In this paper, we applied MAO and steam–hydrothermal treatment (SHT) to produce HA-coated Ti, hereafter called Ti–M–H. The surface morphology of Ti–M–H1 was observed by scanning electron microscopy (SEM), and the element composition and the roughness of Ti–M–H1 were analyzed by energy-dispersive X-ray analysis, an X-ray diffractometer (XRD), and Bruker stylus profiler, demonstrating the deposition of nano-HA particles on Ti surfaces that were composed of Ca, P, Ti, and O. Then, the role of Ti–M–H in osteogenesis and angiogenesis in vitro was evaluated. The data illustrated that Ti–M–H1 showed a good compatibility with osteoblasts (OBs), which promoted adhesion, spreading, and proliferation. Additionally, the secretion of ALP, Col-1, and extracellular matrix mineralization was increased by OBs treated with Ti–M–H1. Ti–M–H1 could stimulate endothelial cells to secrete vascular endothelial growth factor and promote the formation of capillary-like networks. Next, it was revealed that Ti–M–H1 also suppressed inflammation by activating macrophages, while releasing multiple active factors to mediate osteogenesis and angiogenesis. Finally, in vivo results uncovered that Ti–M–H1 facilitated a higher bone-to-implant interface and was more attractive for the dendrites, which promoted osseointegration. In summary, MAO and SHT-treated Ti–M–H1 not only promotes in vitro osteogenesis and angiogenesis but also induces M2 macrophages to regulate the immune environment, which enhances the crosstalk between osteogenesis and angiogenesis and ultimately accelerates the process of osseointegration in vivo.
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Affiliation(s)
- Xiaojun Wang
- Department of Orthopedics, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China.,Department of Orthopedics, Huzhou Traditional Chinese Medicine Hospital, Affiliated to Zhejiang Chinese Medical University, Huzhou, China
| | - Lina Mei
- Internal Medicine, Huzhou Maternity and Child Health Care Hospital, Huzhou, China
| | - Xuesheng Jiang
- Department of Orthopedics, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China
| | - Mingchao Jin
- Department of Orthopedics, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China
| | - Yan Xu
- Department of Rehabilitation, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China
| | - Jianyou Li
- Department of Orthopedics, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China
| | - Xiongfeng Li
- Department of Orthopedics, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China
| | - Zhipeng Meng
- Department of Anaesthesiology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China
| | - Junkun Zhu
- Orthopedics Rehabilitation Department, Lishui Municipal Central Hospital, Lishui, China
| | - Fengfeng Wu
- Department of Orthopedics, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang University Huzhou Hospital, Huzhou, China
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13
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Titanium dioxide nanotubes as drug carriers for infection control and osteogenesis of bone implants. Drug Deliv Transl Res 2021; 11:1456-1474. [PMID: 33942245 DOI: 10.1007/s13346-021-00980-z] [Citation(s) in RCA: 25] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 04/06/2021] [Indexed: 02/07/2023]
Abstract
Titanium implants have been widely used as one of the most effective treatments of bone defects. However, the lack of osteogenesis and bacteria-resistant activities result in high infection and loosening rates of titanium implants. Anodic oxidation could easily construct titanium dioxide nanotubes (TNTs) array on the surface of titanium, and the rough surface of TNTs is beneficial to the growth of osteoblast-related cells on the surface. And TNTs could be excellent drug carriers because of their single-entry tubular hollow structure. In this review, we aim at detailing the application of TNTs as drug carriers in the field of bone implants. Starting from the topography of TNTs, we illustrated the biological activity of the TNTs surface, the drugs for loading in TNTs, and the controlled and responsive release strategies of drug-loaded TNTs, respectively. At the end of this review, the shortcomings of TNTs as the drug carrier in the field of bone implants are discussed, and the development direction of this research field is also prospected.
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14
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Zhang Y, Hu K, Xing X, Zhang J, Zhang MR, Ma X, Shi R, Zhang L. Smart Titanium Coating Composed of Antibiotic Conjugated Peptides as an Infection-Responsive Antibacterial Agent. Macromol Biosci 2021; 21:e2000194. [PMID: 33052007 DOI: 10.1002/mabi.202000194] [Citation(s) in RCA: 18] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/02/2020] [Revised: 08/16/2020] [Indexed: 02/04/2023]
Abstract
Antibacterial coating is rapidly emerging as a pivotal strategy for mitigating spread of bacterial pathogens. However, many challenges still need to be overcome in order to develop a smart coating that can achieve on-demand antibacterial effects. In this study, a Staphylococcus aureus (S. aureus) sensitive peptide sequence is designed, and an antibiotic is then conjugated with this tailor-made peptide. The antibiotic-peptide conjugate is then linked to the surface of a titanium implant, where the peptide can be recognized and cleaved by an enzyme secreted by S. aureus. This allows for the release of antibiotics in the presence of S. aureus, thus achieving delivery of an antibacterial specifically when an infection occurs.
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Affiliation(s)
- Yunfei Zhang
- Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, P. R. China
| | - Kuan Hu
- Department of Advanced Nuclear Medicine Sciences, National Institute of Quantum Sciences and Technologies, Chiba, 263-8555, Japan
| | - Xuan Xing
- Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, P. R. China
| | - Jingshuang Zhang
- Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing Laboratory of Biomedical Materials, Beijing, 100035, P. R. China
| | - Ming-Rong Zhang
- Department of Advanced Nuclear Medicine Sciences, National Institute of Quantum Sciences and Technologies, Chiba, 263-8555, Japan
| | - Xiaohui Ma
- Department of Vascular Surgery, General Hospital of People's Liberation Army, Beijing, 100853, P. R. China
| | - Rui Shi
- Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing Laboratory of Biomedical Materials, Beijing, 100035, P. R. China
| | - Liqun Zhang
- Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, P. R. China
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15
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Pantaroto HN, Cordeiro JM, Pereira LT, de Almeida AB, Nociti Junior FH, Rangel EC, Azevedo Neto NF, da Silva JHD, Barão VAR. Sputtered crystalline TiO 2 film drives improved surface properties of titanium-based biomedical implants. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2020; 119:111638. [PMID: 33321676 DOI: 10.1016/j.msec.2020.111638] [Citation(s) in RCA: 17] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/01/2020] [Revised: 09/17/2020] [Accepted: 10/13/2020] [Indexed: 12/13/2022]
Abstract
Different crystalline phases in sputtered TiO2 films were tailored to determine their surface and electrochemical properties, protein adsorption and apatite layer formation on titanium-based implant material. Deposition conditions of two TiO2 crystalline phases (anatase and rutile) were established and then grown on commercially pure titanium (cpTi) by magnetron sputtering to obtain the following groups: A-TiO2 (anatase), M-TiO2 (anatase and rutile mixture), R-TiO2 (rutile). Non-treated commercially pure titanium (cpTi) was used as a control. Surfaces characterization included: chemical composition, topography, crystalline phase and surface free energy (SFE). Electrochemical tests were conducted using simulated body fluid (SBF). Albumin adsorption was measured by bicinchoninic acid method. Hydroxyapatite (HA) precipitation was evaluated after 28 days of immersion in SBF. MC3T3-E1 cell adhesion, morphology and spreading onto the experimental surfaces were evaluated by scanning electron microscopy. Sputtering treatment modified cpTi topography by increasing its surface roughness. CpTi and M-TiO2 groups presented the greatest SFE. In general, TiO2 films displayed improved electrochemical behavior compared to cpTi, with M-TiO2 featuring the highest polarization resistance. Rutile phase exhibited a greater influence on decreasing the current density and corrosion rate, while the presence of a bi-phasic polycrystalline condition displayed a more stable passive behavior. M-TiO2 featured increased albumin adsorption. HA morphology was dependent on the crystalline phase, being more evident in the bi-phasic group. Furthermore, M-TiO2 displayed normal cell adhesion and morphology. The combination of anatase and rutile structures to generate TiO2 films is a promising strategy to improve biomedical implants properties including greater corrosion protection, higher protein adsorption, bioactivity and non-cytotoxicity effect.
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Affiliation(s)
- Heloisa Navarro Pantaroto
- University of Campinas (UNICAMP), Piracicaba Dental School, Department of Prosthodontics and Periodontics, Av. Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
| | - Jairo Matozinho Cordeiro
- University of Campinas (UNICAMP), Piracicaba Dental School, Department of Prosthodontics and Periodontics, Av. Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil; Institute of Biomaterials, Tribocorrosion and Nanomedicine (IBTN), Brazil
| | - Lucas Toniolo Pereira
- University of Campinas (UNICAMP), Piracicaba Dental School, Department of Prosthodontics and Periodontics, Av. Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
| | - Amanda Bandeira de Almeida
- University of Campinas (UNICAMP), Piracicaba Dental School, Department of Prosthodontics and Periodontics, Av. Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
| | - Francisco Humberto Nociti Junior
- University of Campinas (UNICAMP), Piracicaba Dental School, Department of Prosthodontics and Periodontics, Av. Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
| | - Elidiane Cipriano Rangel
- São Paulo State University (UNESP), Institute of Science and Technology, Av. Três de Março, 511, Sorocaba, São Paulo, 18087-180, Brazil
| | - Nilton Francelosi Azevedo Neto
- São Paulo State University (UNESP), Department of Physics, Av. Eng. Luís Edmundo C. Coube, 14-01, Bauru, São Paulo 17033-360, Brazil
| | - Jose Humberto Dias da Silva
- São Paulo State University (UNESP), Department of Physics, Av. Eng. Luís Edmundo C. Coube, 14-01, Bauru, São Paulo 17033-360, Brazil
| | - Valentim Adelino Ricardo Barão
- University of Campinas (UNICAMP), Piracicaba Dental School, Department of Prosthodontics and Periodontics, Av. Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil; Institute of Biomaterials, Tribocorrosion and Nanomedicine (IBTN), Brazil.
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16
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Effect of TiO 2 Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts. NANOMATERIALS 2020; 10:nano10112117. [PMID: 33113757 PMCID: PMC7692029 DOI: 10.3390/nano10112117] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/16/2020] [Revised: 10/13/2020] [Accepted: 10/20/2020] [Indexed: 12/18/2022]
Abstract
The pore diameter of uniformly structured nanotubes can significantly change the behaviour of cells. Recent studies demonstrated that the activation of integrins is affected not by only the surface chemistry between the cell-material interfaces, but also by the features of surface nanotopography, including nanotube diameter. While research has been carried out in this area, there has yet to be a single systemic study to date that succinctly compares the response of both human stem cells and osteoblasts to a range of TiO2 nanotube pore diameters using controlled experiments in a single laboratory. In this paper, we investigate the influence of surface nanotopography on cellular behaviour and osseointegrative properties through a systemic study involving human mesenchymal stem cells (hMSCs) and human osteoblasts (HOBs) on TiO2 nanotubes of 20 nm, 50 nm and 100 nm pore diameters using in-vitro assessments. This detailed study demonstrates the interrelationship between cellular behaviour and nanotopography, revealing that a 20 nm nanotube pore diameter is preferred by hMSCs for the induction of osteogenic differentiation, while 50 nm nanotubular structures are favourable by HOBs for osteoblastic maturation.
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17
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Tao F, Ma S, Tao H, Jin L, Luo Y, Zheng J, Xiang W, Deng H. Chitosan-based drug delivery systems: From synthesis strategy to osteomyelitis treatment - A review. Carbohydr Polym 2020; 251:117063. [PMID: 33142615 DOI: 10.1016/j.carbpol.2020.117063] [Citation(s) in RCA: 78] [Impact Index Per Article: 15.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/08/2020] [Revised: 08/22/2020] [Accepted: 09/03/2020] [Indexed: 12/21/2022]
Abstract
Osteomyelitis is a complex disease in orthopedics mainly caused by bacterial pathogens invading bone or bone marrow. The treatment of osteomyelitis is highly difficult and it is a major challenge in orthopedic surgery. The long-term systemic use of antibiotics may lead to antibiotic resistance and has limited effects on eradicating local biofilms. Localized antibiotic delivery after surgical debridement can overcome the problem of antibiotic resistance and reduce systemic toxicity. Chitosan, a special cationic polysaccharide, is a product extracted from the deacetylation of chitin. It has numerous advantages, such as nontoxicity, biocompatibility, and biodegradability. Recently, chitosan has attracted significant attention in bacterial inhibition and drug delivery. Because chitosan contains many functional bioactive groups conducive to chemical reaction and modification, some chitosan-based biomaterials have been applied as the local antibiotic delivery systems in the treatment of osteomyelitis. This review aims to introduce recent advances in the biomedical applications of chitosan-based drug delivery systems in osteomyelitis treatment and to highlight the perspectives for further studies.
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Affiliation(s)
- Fenghua Tao
- Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China; Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China
| | - Sijia Ma
- Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China
| | - Hai Tao
- Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China
| | - Lin Jin
- Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China
| | - Yue Luo
- Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China
| | - Jian Zheng
- Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China
| | - Wei Xiang
- Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China; Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
| | - Hongbing Deng
- Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
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18
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Innovative Nanoporous Titania Surface with Stabilized Antimicrobial Ag-Nanoparticles via Salvadora persica L. Roots (Miswak) Extract for Dental Applications. BIONANOSCIENCE 2020. [DOI: 10.1007/s12668-020-00765-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/23/2022]
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19
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Huo SC, Yue B. Approaches to promoting bone marrow mesenchymal stem cell osteogenesis on orthopedic implant surface. World J Stem Cells 2020; 12:545-561. [PMID: 32843913 PMCID: PMC7415248 DOI: 10.4252/wjsc.v12.i7.545] [Citation(s) in RCA: 24] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/28/2020] [Revised: 05/13/2020] [Accepted: 05/30/2020] [Indexed: 02/06/2023] Open
Abstract
Bone marrow-derived mesenchymal stem cells (BMSCs) play a critical role in the osseointegration of bone and orthopedic implant. However, osseointegration between the Ti-based implants and the surrounding bone tissue must be improved due to titanium’s inherent defects. Surface modification stands out as a versatile technique to create instructive biomaterials that can actively direct stem cell fate. Here, we summarize the current approaches to promoting BMSC osteogenesis on the surface of titanium and its alloys. We will highlight the utilization of the unique properties of titanium and its alloys in promoting tissue regeneration, and discuss recent advances in understanding their role in regenerative medicine. We aim to provide a systematic and comprehensive review of approaches to promoting BMSC osteogenesis on the orthopedic implant surface.
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Affiliation(s)
- Shi-Cheng Huo
- Department of Bone and Joint Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
| | - Bing Yue
- Department of Bone and Joint Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
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20
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Wang Q, Zhou P, Liu S, Attarilar S, Ma RLW, Zhong Y, Wang L. Multi-Scale Surface Treatments of Titanium Implants for Rapid Osseointegration: A Review. NANOMATERIALS (BASEL, SWITZERLAND) 2020; 10:E1244. [PMID: 32604854 PMCID: PMC7353126 DOI: 10.3390/nano10061244] [Citation(s) in RCA: 89] [Impact Index Per Article: 17.8] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/05/2020] [Revised: 05/30/2020] [Accepted: 06/22/2020] [Indexed: 02/06/2023]
Abstract
The propose of this review was to summarize the advances in multi-scale surface technology of titanium implants to accelerate the osseointegration process. The several multi-scaled methods used for improving wettability, roughness, and bioactivity of implant surfaces are reviewed. In addition, macro-scale methods (e.g., 3D printing (3DP) and laser surface texturing (LST)), micro-scale (e.g., grit-blasting, acid-etching, and Sand-blasted, Large-grit, and Acid-etching (SLA)) and nano-scale methods (e.g., plasma-spraying and anodization) are also discussed, and these surfaces are known to have favorable properties in clinical applications. Functionalized coatings with organic and non-organic loadings suggest good prospects for the future of modern biotechnology. Nevertheless, because of high cost and low clinical validation, these partial coatings have not been commercially available so far. A large number of in vitro and in vivo investigations are necessary in order to obtain in-depth exploration about the efficiency of functional implant surfaces. The prospective titanium implants should possess the optimum chemistry, bionic characteristics, and standardized modern topographies to achieve rapid osseointegration.
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Affiliation(s)
- Qingge Wang
- School of Metallurgical Engineering, Xi’an University of Architecture and Technology, No.13 Yanta Road, Xi’an 710055, China;
| | - Peng Zhou
- School of Aeronautical Materials Engineering, Xi’an Aeronautical Polytechnic Institute, Xi’an 710089, China;
| | - Shifeng Liu
- School of Metallurgical Engineering, Xi’an University of Architecture and Technology, No.13 Yanta Road, Xi’an 710055, China;
| | - Shokouh Attarilar
- State Key Laboratory of Metal Matrix Composites, School of Material Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
| | - Robin Lok-Wang Ma
- Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Hong Kong 999077, China; (R.L.-W.M.); (Y.Z.)
| | - Yinsheng Zhong
- Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Hong Kong 999077, China; (R.L.-W.M.); (Y.Z.)
| | - Liqiang Wang
- State Key Laboratory of Metal Matrix Composites, School of Material Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
- National Engineering Research Center for Nanotechnology (NERCN), 28 East JiangChuan Road, Shanghai 200241, China
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21
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Gao Q, Feng T, Huang D, Liu P, Lin P, Wu Y, Ye Z, Ji J, Li P, Huang W. Antibacterial and hydroxyapatite-forming coating for biomedical implants based on polypeptide-functionalized titania nanospikes. Biomater Sci 2020; 8:278-289. [PMID: 31691698 DOI: 10.1039/c9bm01396b] [Citation(s) in RCA: 52] [Impact Index Per Article: 10.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/08/2023]
Abstract
Titanium (Ti)-based implants often suffer from detrimental bacterial adhesion and inefficient healing, so it is crucial to design a dual-functional coating that prevents bacterial infection and enhances bioactivity for a successful implant. Herein, we successfully devised a cationic polypeptide (Pep)-functionalized biomimetic nanostructure coating with superior activity, which could not only kill pathogenic bacteria rapidly and inhibit biofilm formation for up to two weeks, but also promote in situ hydroxyapatite (HAp) formation. Specifically, a titania (TiO2) nanospike coating (TNC) was fabricated by alkaline hydrothermal treatment firstly, followed by immobilization of rationally synthesized Pep via robust coordinative interactions, named TNPC. This coating was able to effectively kill (>99.9%) both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria, while being non-toxic to murine MC3T3-E1 osteoblastic cells. Furthermore, the in vivo infection studies denoted that the adherent bacteria numbers on the TNPC implants were significantly reduced by 6 orders of magnitude than those on the pure Ti implants (p < 0.001). Importantly, in the presence of cationic amino groups and residual Ti-OH groups, substantial HAp deposition on the TNPC surface in Kokubo's simulated body fluid (SBF) occurred after 14 days. Altogether, our results support the clinical potential of this biomimetic dual-functional coating as a new approach with desirable antibacterial properties and HAp-forming ability in orthopedic and dental applications.
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Affiliation(s)
- Qiang Gao
- MOE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
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22
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Ozan S, Munir K, Biesiekierski A, Ipek R, Li Y, Wen C. Titanium Alloys, Including Nitinol. Biomater Sci 2020. [DOI: 10.1016/b978-0-12-816137-1.00018-0] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/24/2022]
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23
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Yu Y, Ran Q, Shen X, Zheng H, Cai K. Enzyme responsive titanium substrates with antibacterial property and osteo/angio-genic differentiation potentials. Colloids Surf B Biointerfaces 2019; 185:110592. [PMID: 31639570 DOI: 10.1016/j.colsurfb.2019.110592] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/07/2019] [Revised: 10/12/2019] [Accepted: 10/14/2019] [Indexed: 12/11/2022]
Abstract
After implantation into a host, titanium (Ti) orthopaedic materials are facing two major clinical challenges: bacterial infection and aseptic loosening, which directly determine the long-term survival of the implant. To endow Ti implant with self-defensive antibacterial properties and desirable osteo/angio-genic differentiation potentials, hyaluronic acid (HA)-gentamicin (Gen) conjugates (HA-Gen) and chitosan (Chi) polyelectrolyte multilayers were constructed on deferoxamine (DFO) loaded titania nanotubes (TNT) substrates via layer-by-layer (LBL) assembly technique, termed as TNT/DFO/HA-Gen. The HA-Gen conjugate was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (1H NMR). The physicochemical properties of the substrates were characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The on-demand DFO release was associated with the degradation of multilayers triggered by exogenous hyaluronidase, which indicated enzymatic and bacterial responsiveness. The TNT/DFO/HA-Gen substrates displayed effective antifouling and antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), while were favourable for the adhesion, proliferation and osteo/angio-genic differentiation of mesenchymal stem cells (MSCs). The multifaceted drug-device combination (DDC) strategy showed potential applications in orthopaedic fields.
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Affiliation(s)
- Yonglin Yu
- Department of Pathology, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China.
| | - Qichun Ran
- School of Communication and Information Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China
| | - Xinkun Shen
- School of Life Science, Chongqing University, Chongqing 400044, China
| | - Hong Zheng
- Department of Pathology, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China.
| | - Kaiyong Cai
- Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
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Lu RJ, Wang X, He HX, E LL, Li Y, Zhang GL, Li CJ, Ning CY, Liu HC. Tantalum-incorporated hydroxyapatite coating on titanium implants: its mechanical and in vitro osteogenic properties. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE 2019; 30:111. [PMID: 31583537 DOI: 10.1007/s10856-019-6308-9] [Citation(s) in RCA: 22] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/28/2019] [Accepted: 08/30/2019] [Indexed: 06/10/2023]
Abstract
OBJECTIVE The fabrication of bioactive coatings on metallic implants to enhance osseointegration has become a topic of general interest in orthopedics and dentistry. Hydroxyapatite (HA) coating has been shown to induce bone formation and promote bone-implant integration. Unfortunately, poor mechanical performance has hindered this from becoming a favorable coating material. The majority of present studies have focused in incorporating different elements into HA coatings to improve mechanical properties. In recent years, tantalum (Ta) has received increasing attention due to its excellent biocompatibility and corrosion resistance. The aim of on the present study was to investigate the fabrication and biological performance of Ta-incorporated HA coatings. METHODS Ta-incorporated HA coatings were fabricated using the plasma spray technique on a titanium substrate, and the surface characteristics and mechanical properties were examined. In addition, the effects of Ta-incorporated HA coatings on the biological behavior of mesenchymal stem cells (BMSCs) were investigated. RESULTS Ta-incorporated HA coatings with microporous structure had higher roughness and wettability. In addition, the bonding strength of Ta/HA coatings with the substrate was substantially superior to HA coatings. Furthermore, Ta-incorporated HA coatings not only facilitated initial cell adhesion and faster proliferation, but also promoted the osteogenic differentiation of BMSCs. CONCLUSION These results indicate that the incorporation of Ta could improve mechanical performance and increase the osteogenic activity of HA coatings. The Ta-incorporated HA coating fabricated by plasma spraying is expected to be a promising bio-coating material for metallic implants.
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Affiliation(s)
- Rong-Jian Lu
- Department of Stomatology, the Fifth Medical Center, Chinese PLA General Hospital, 100071, Beijing, China
- Department of Stomatology, the First Medical Center, Chinese PLA General Hospital, 100853, Beijing, China
| | - Xing Wang
- Shanxi Medical University School and Hospital of Stomatology, 030001, Taiyuan, China
| | - Hui-Xia He
- Department of Stomatology, the First Medical Center, Chinese PLA General Hospital, 100853, Beijing, China
| | - Ling-Ling E
- Department of Stomatology, the First Medical Center, Chinese PLA General Hospital, 100853, Beijing, China
| | - Ying Li
- School of Materials Science and Technology, South China University of Technology, 510641, Guangzhou, China
| | - Gui-Lan Zhang
- Department of Stomatology, the First Medical Center, Chinese PLA General Hospital, 100853, Beijing, China
| | - Chuan-Jie Li
- Department of Stomatology, the First Medical Center, Chinese PLA General Hospital, 100853, Beijing, China
| | - Cheng-Yun Ning
- School of Materials Science and Technology, South China University of Technology, 510641, Guangzhou, China
| | - Hong-Chen Liu
- Department of Stomatology, the First Medical Center, Chinese PLA General Hospital, 100853, Beijing, China.
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25
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Li Y, Yang Y, Li R, Tang X, Guo D, Qing Y, Qin Y. Enhanced antibacterial properties of orthopedic implants by titanium nanotube surface modification: a review of current techniques. Int J Nanomedicine 2019; 14:7217-7236. [PMID: 31564875 PMCID: PMC6733344 DOI: 10.2147/ijn.s216175] [Citation(s) in RCA: 53] [Impact Index Per Article: 8.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/17/2019] [Accepted: 08/09/2019] [Indexed: 11/23/2022] Open
Abstract
Prosthesis-associated infections are one of the main causes of implant failure; thus it is important to enhance the long-term antibacterial ability of orthopedic implants. Titanium dioxide nanotubes (TNTs) are biomaterials with good physicochemical properties and biocompatibility. Owing to their inherent antibacterial and drug-loading ability, the antibacterial application of TNTs has received increasing attention. In this review, the process of TNT anodizing fabrication is summarized. Also, the mechanism and the influencing factors of the antibacterial property of bare TNTs are explored. Furthermore, different antibacterial strategies for carrying drugs, as well as modifications to prolong the antibacterial effect and reduce drug-related toxicity are discussed. In addition, antibacterial systems based on TNTs that can automatically respond to infection are introduced. Finally, the currently faced problems are reviewed and potential solutions are proposed. This review provides new insight on TNT fabrication and summarizes the most advanced antibacterial strategies involving TNTs for the enhancement of long-term antibacterial ability and reduction of toxicity.
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Affiliation(s)
- Yuehong Li
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun, People’s Republic of China
| | - Yue Yang
- Department of Cardiology, China-Japan Union Hospital of Jilin University, Changchun, People’s Republic of China
| | - Ruiyan Li
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun, People’s Republic of China
| | - Xiongfeng Tang
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun, People’s Republic of China
| | - Deming Guo
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun, People’s Republic of China
| | - Yun'an Qing
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun, People’s Republic of China
| | - Yanguo Qin
- Orthopaedic Medical Center, The Second Hospital of Jilin University, Changchun, People’s Republic of China
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Kaur M, Singh K. Review on titanium and titanium based alloys as biomaterials for orthopaedic applications. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2019; 102:844-862. [PMID: 31147056 DOI: 10.1016/j.msec.2019.04.064] [Citation(s) in RCA: 436] [Impact Index Per Article: 72.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/12/2018] [Revised: 02/20/2019] [Accepted: 04/20/2019] [Indexed: 02/07/2023]
Abstract
Variety of implant materials have been employed in various disciplines of medical science depending on the requirement of a particular application. Metals, alloys, ceramics, and polymers are the commonly used biomaterials. The main focus of this study is to review the various structural and microstructural properties of titanium and titanium based alloys used as orthopaedic implants. Orthopaedic implants need to possess certain important qualities to ensure their safe and effective use. These properties like the biocompatibility, relevant mechanical properties, high corrosion and wear resistance and osseointegration are summarized in this review. Various attempts to improve upon these properties like different processing routes, surface modifications have also been inculcated in the paper to provide an insight into the extent of research and effort that has been put into developing a highly superior titanium orthopaedic implant.
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Affiliation(s)
- Manmeet Kaur
- School of Physics and Materials Science, Thapar Institute of Engineering and Technology, Patiala, Punjab 147004, India
| | - K Singh
- School of Physics and Materials Science, Thapar Institute of Engineering and Technology, Patiala, Punjab 147004, India.
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27
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Zhang M, Zeng G, Liao X, Wang Y. An antibacterial and biocompatible piperazine polymer. RSC Adv 2019; 9:10135-10147. [PMID: 35520902 PMCID: PMC9062374 DOI: 10.1039/c9ra02219h] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/22/2019] [Accepted: 03/25/2019] [Indexed: 12/20/2022] Open
Abstract
Bacterial repellence by biomedical materials is a desirable property that can potentially improve the healing process. In this study, we described a simple and green method to prepare a novel piperazine polymer (PE), which was based on the raw materials piperazine (PA) and ethylenediaminetetraacetic dianhydride (EDTAD). The structure and thermal stability of the obtained material were characterized using Fourier transform infrared spectrometry (FTIR), nuclear magnetic resonance spectroscopy (NMR), elementary analysis, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). To evaluate the antibacterial properties of PE, a strain of Gram-negative Escherichia coli (E. coli) bacteria and a strain of Gram-positive Staphylococcus aureus (S. aureus) bacteria were used. The results indicated that PE exhibited good antibacterial activity against both strains of bacteria in a short time frame. The initial cytotoxicity test of the obtained material was based on the changes in the morphology and proliferation of osteoblasts, and the results demonstrated that the cytotoxicity of PE was concentration-dependent. Combining the experimental results of these two parts, it was shown that bacteria could be inhibited by a certain concentration of PE, while its toxicity toward osteoblasts was very low. In summary, these results revealed the potential usefulness of PE in biomedical applications.
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Affiliation(s)
- Maolan Zhang
- Chongqing University of Science and Technology Chongqing 401331 China +86 17830862118 +86 17830862118
| | - Guoming Zeng
- Chongqing University of Science and Technology Chongqing 401331 China +86 17830862118 +86 17830862118
| | - Xiaoling Liao
- Chongqing University of Science and Technology Chongqing 401331 China +86 17830862118 +86 17830862118
| | - Yuanliang Wang
- Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education Chongqing 400044 China
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28
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Surface Immobilization of TiO 2 Nanotubes with Bone Morphogenetic Protein-2 Synergistically Enhances Initial Preosteoblast Adhesion and Osseointegration. BIOMED RESEARCH INTERNATIONAL 2019; 2019:5697250. [PMID: 31032352 PMCID: PMC6457305 DOI: 10.1155/2019/5697250] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/16/2018] [Accepted: 03/10/2019] [Indexed: 11/18/2022]
Abstract
Although titanium (Ti) alloys have been widely used as implant materials, the bioinertness of pristine Ti impairs their bioactivity and early osseointegration. In the present work, we prepared TiO2 nanotubes (TNT) layer on the titanium (Ti) surface by anodic oxidation. The anodized surface was functionalized with human bone morphogenetic protein-2 coating to form the hBMP-2/TNT surface. The release behavior of hBMP-2 on the hBMP-2/TNT surface displayed a controlled and sustained pattern, compared to that on the hBMP-2/Ti surface, which showed a rapid release. In vitro cellular activity tests demonstrated that both TNT and hBMP-2/Ti surfaces, particularly the hBMP-2/TNT surface, enhanced adhesion, proliferation, and differentiation of osteoblast cells. Increased cell adhesion, improved cytoskeleton organization, and immunofluorescence staining of vinculin were observed on the modified surfaces. The TNT, hBMP-2/Ti, and hBMP-2/TNT surfaces, especially the hBMP-2/TNT surface, further displayed an upregulated gene expression of adhesion and osteogenic markers vinculin, collagen type 1, osteopontin, and osteocalcin, compared to the pristine Ti surface. In vivo experiments using a rat model demonstrated that the TNT and hBMP-2/Ti surfaces, in particular the hBMP-2/TNT surface, improved osseointegration and showed a superior bone bonding ability compared to Ti. Our study revealed a synergistic role played by TiO2 nanotubes nanotopography and hBMP-2 in promoting initial osteoblast adhesion, proliferation, differentiation, and osseointegration, thus suggesting a promising method for better modifying the implant surface.
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29
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Huang L, Chen J, Li X, Liu H, Li J, Ren T, Yang Y, Zhong S. Polymethacrylic acid encapsulated TiO2 nanotubes for sustained drug release and enhanced antibacterial activities. NEW J CHEM 2019. [DOI: 10.1039/c8nj04568b] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
A long-term antibacterial and sustained drug release system was fabricated, in which the TNTs acted as the loading platform of NOR and then encapsulated with PMAA.
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Affiliation(s)
- Ling Huang
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
| | - Jian Chen
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
| | - Xiufang Li
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
| | - Hui Liu
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
| | - Jianbing Li
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
| | - Tao Ren
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
| | - Yanjing Yang
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
| | - Shian Zhong
- College of Chemistry and Chemical Engineering
- Central South University
- Changsha Hunan
- P. R. China
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30
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Santos-Coquillat A, Mohedano M, Martinez-Campos E, Arrabal R, Pardo A, Matykina E. Bioactive multi-elemental PEO-coatings on titanium for dental implant applications. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2018; 97:738-752. [PMID: 30678963 DOI: 10.1016/j.msec.2018.12.097] [Citation(s) in RCA: 24] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/25/2018] [Revised: 11/28/2018] [Accepted: 12/25/2018] [Indexed: 01/22/2023]
Abstract
Bioactive PEO (Plasma Electrolytic Oxidation) coatings were generated on Grade I commercially pure titanium for dentistry applications using a Ca/P-based electrolyte with added Si, Mg, Zn or F species. Surface characteristics, chemical composition and ion liberation of the coatings were characterized using SEM/EDS, X-ray diffraction, optical profilometry, contact angle and ICP-OES. Corrosion resistance (OCP and DC polarization) was evaluated in SBF. Osteoblastogenesis and osteoclastogenesis processes on PEO-coated Ti and non-coated Ti controls were assessed after 7 days and 5 days of cell culture, respectively. Monolayer formation and metabolic activity were evaluated for the MC3T3 preosteoblastic cell line. All PEO coatings favoured differentiation processes over proliferation and presented three times greater quantity of secreted collagen than non-coated Ti control. All coating enabled osteoclast differentiation, with differences in number and size of the osteoclasts between the materials.
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Affiliation(s)
- A Santos-Coquillat
- Departamento de Ingenieria Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain; Tissue Engineering Group, Institute of Biofunctional Studies (IEB-UCM), Associated Unit to the Institute of Polymer Science and Technology (CSIC), Polymer Functionalization Group, 28040 Madrid, Spain.
| | - M Mohedano
- Departamento de Ingenieria Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain
| | - E Martinez-Campos
- Tissue Engineering Group, Institute of Biofunctional Studies (IEB-UCM), Associated Unit to the Institute of Polymer Science and Technology (CSIC), Polymer Functionalization Group, 28040 Madrid, Spain; Institute of Biofunctional Studies of Complutense University of Madrid (IEB-UCM), Paseo Juan XXIII, 1, 28040 Madrid, Spain
| | - R Arrabal
- Departamento de Ingenieria Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain
| | - A Pardo
- Departamento de Ingenieria Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain
| | - E Matykina
- Departamento de Ingenieria Química y de Materiales, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain; Institute of Biofunctional Studies of Complutense University of Madrid (IEB-UCM), Paseo Juan XXIII, 1, 28040 Madrid, Spain
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31
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Yuan Z, Liu P, Hao Y, Ding Y, Cai K. Construction of Ag-incorporated coating on Ti substrates for inhibited bacterial growth and enhanced osteoblast response. Colloids Surf B Biointerfaces 2018; 171:597-605. [DOI: 10.1016/j.colsurfb.2018.07.064] [Citation(s) in RCA: 22] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/19/2018] [Revised: 07/03/2018] [Accepted: 07/27/2018] [Indexed: 10/28/2022]
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Liu P, Hao Y, Ding Y, Yuan Z, Liu Y, Cai K. Fabrication of enzyme-responsive composite coating for the design of antibacterial surface. JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE 2018; 29:160. [PMID: 30350231 DOI: 10.1007/s10856-018-6171-0] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/10/2018] [Accepted: 10/12/2018] [Indexed: 06/08/2023]
Abstract
In this study, a type of bacteria enzyme-triggered antibacterial surface with a controlled release of Ag ions was developed. Firstly, chitosan-silver nanocomposites (Chi@Ag NPs) were in situ synthesized via using ascorbic acid as reducing agent. Chi@Ag NPs were characterized by transmission electron microscopy, ultraviolet-visible spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Subsequently, Chi@Ag NPs and hyaluronic acid (HA) were used to fabricate antibacterial composite coating via Layer-by-Layer (LBL) self-assembly method. The successful construction of Chi@Ag NPs/HA composite coating was confirmed by scanning electron microscopy, energy dispersive spectroscopy and contact angle measurements, respectively. Then, the amount of released Ag ion was analyzed by inductively coupled plasma atomic emission spectrometry, which demonstrated that the release of Ag ions from the surface could be triggered by enzyme (e.g. hyaluronidase). A series of antibacterial tests in vitro, including zone of inhibition test, bacterial viability assay, antibacterial rate measurement and bacteria adhesion observation, demonstrated that the enzyme-responsive surface could inhibit the growth of bacteria. On the whole, this study provides an alternative approach for the fabrication of antibacterial surfaces on synthetic materials in various fields with the minimal side effects on surrounding environment and human body.
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Affiliation(s)
- Peng Liu
- Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, 400044, Chongqing, China.
| | - Yansha Hao
- Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, 400044, Chongqing, China
| | - Yao Ding
- Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, 400044, Chongqing, China
| | - Zhang Yuan
- Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, 400044, Chongqing, China
| | - Yisi Liu
- Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, 400044, Chongqing, China
| | - Kaiyong Cai
- Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, 400044, Chongqing, China.
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Liu J, Yang W, Tao B, Shen T, He Y, Shen X, Cai K. Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface. J Biomed Mater Res A 2018; 106:3021-3033. [DOI: 10.1002/jbm.a.36491] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/18/2018] [Revised: 06/14/2018] [Accepted: 06/18/2018] [Indexed: 12/15/2022]
Affiliation(s)
- Ju Liu
- Key Laboratory of Biorheological Science and Technology; Ministry of Education, College of Bioengineering, Chongqing University; Chongqing, 400044 China
| | - Weihu Yang
- Key Laboratory of Biorheological Science and Technology; Ministry of Education, College of Bioengineering, Chongqing University; Chongqing, 400044 China
| | - Bailong Tao
- Key Laboratory of Biorheological Science and Technology; Ministry of Education, College of Bioengineering, Chongqing University; Chongqing, 400044 China
| | - Tingting Shen
- Key Laboratory of Biorheological Science and Technology; Ministry of Education, College of Bioengineering, Chongqing University; Chongqing, 400044 China
| | - Ye He
- Key Laboratory of Biorheological Science and Technology; Ministry of Education, College of Bioengineering, Chongqing University; Chongqing, 400044 China
| | - Xinkun Shen
- Key Laboratory of Biorheological Science and Technology; Ministry of Education, College of Bioengineering, Chongqing University; Chongqing, 400044 China
- School of Life Science; Chongqing University; Chongqing, 400044 People's Republic of China
| | - Kaiyong Cai
- Key Laboratory of Biorheological Science and Technology; Ministry of Education, College of Bioengineering, Chongqing University; Chongqing, 400044 China
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Huang Z, Wu Z, Ma B, Yu L, He Y, Xu D, Wu Y, Wang H, Qiu G. Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe 3O 4 nanoparticles for hBMSCs. ROYAL SOCIETY OPEN SCIENCE 2018; 5:172033. [PMID: 30224987 PMCID: PMC6124053 DOI: 10.1098/rsos.172033] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/05/2017] [Accepted: 06/27/2018] [Indexed: 05/14/2023]
Abstract
Titanium (Ti) is an ideal bone substitute due to its superior bio-compatibility and remarkable corrosion resistance. However, in order to improve the osteoconduction and osteoinduction capacities in clinical applications, different kinds of surface modifications are typically applied to Ti alloys. In this study, we fabricated a tightly attached polydopamine-assisted Fe3O4 nanoparticle coating on Ti with magnetic properties, aiming to improve the osteogenesis of the Ti substrates. The PDA-assisted Fe3O4 nanoparticle coatings were characterized by scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy and water contact angle measurements. The cell attachment and proliferation rate of the human bone mesenchymal stem cells (hBMSCs) on the Ti surface significantly improved with the Fe3O4/PDA coating when compared with the pure Ti without a coating. Furthermore, the results of in vitro alkaline phosphatase (ALP) activity at 7 and 14 days and alizarin red S staining at 14 days showed that the Fe3O4/PDA coating on Ti promoted the osteogenic differentiation of hBMSCs. Moreover, hBMSCs co-cultured with the Fe3O4/PDA-coated Ti for approximately 14 days also exhibited a significantly higher mRNA expression level of ALP, osteocalcin and runt-related transcription factor-2 (RUNX2). Our in vitro results revealed that the present PDA-assisted Fe3O4 nanoparticle surface coating is an innovative method for Ti surface modification and shows great potential for clinical applications.
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Affiliation(s)
- Zhenfei Huang
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Zhihong Wu
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
- Central Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Bupeng Ma
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
- Central Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Lingjia Yu
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Yu He
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Derong Xu
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Yuanhao Wu
- Central Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Hai Wang
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
| | - Guixing Qiu
- Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China
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35
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Antibacterial photocatalytic activity of different crystalline TiO2 phases in oral multispecies biofilm. Dent Mater 2018; 34:e182-e195. [DOI: 10.1016/j.dental.2018.03.011] [Citation(s) in RCA: 53] [Impact Index Per Article: 7.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/13/2017] [Revised: 03/23/2018] [Accepted: 03/23/2018] [Indexed: 11/23/2022]
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36
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Yuan Z, Huang S, Lan S, Xiong H, Tao B, Ding Y, Liu Y, Liu P, Cai K. Surface engineering of titanium implants with enzyme-triggered antibacterial properties and enhanced osseointegrationin vivo. J Mater Chem B 2018; 6:8090-8104. [DOI: 10.1039/c8tb01918e] [Citation(s) in RCA: 36] [Impact Index Per Article: 5.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/02/2023]
Abstract
A catechol-functionalized coating on a Van-loaded Ti implant achieves enhanced osseointegration and effective inhibition of bacterial adhesion and enzyme-triggered antibacterial drug release.
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Affiliation(s)
- Zhang Yuan
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Suzhou Huang
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Shaoxiong Lan
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Haizhou Xiong
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Bailong Tao
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Yao Ding
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Yisi Liu
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Peng Liu
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| | - Kaiyong Cai
- Key Laboratory of Biorheological Science and Technology of Ministry of Education
- College of Bioengineering
- Chongqing University
- Chongqing 400044
- China
| |
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