GADD45β在肝癌中的异常表达

表1 部分肝癌中下调表达的基因

UniGene ID	Gene description	CL	Ratio
Hs.75678	FBJ murine osteosarcoma viral oncogene homolog B, FOSB	19q13.32	0.067
Hs.110	solute carrier family 3 member 1, SLC3A1	2p16.3	0.102
Hs.82120	nuclear receptor subfamily 4, group A, member 2, NR4A2	2q22-q23	0.198
Hs.25647	v-fos FBJ murine osteosarcoma viral oncogene homolog, FOS	14q24.3	0.203
Hs.534337	major histocompatibility complex, class II, DR beta 4, HLA-DRB4	6p21.3	0.250
Hs.332053	serum amyloid A1, SAA1	11p15.1	0.287
Hs.1395	early growth response 2, EGR2	10q21.1	0.330
Hs.388674	heart alpha-kinase, HAK	18q21.31	0.354
Hs.171695	dual specificity phosphatase 1, DUSP1	5q34	0.366
Hs.414795	serine (or cysteine) proteinase inhibitor clade E member 1, SERPINE1	7q21.3-q22	0.379
Hs.110571	growth arrest and DNA-damage-inducible, beta GADD45β	chr19p13.3	0.397
Hs.8364	pyruvate dehydrogenase kinase, isoenzyme 4, PDK4	7q21.3-q22.1	0.420
Hs.156637	Cas-Br-M ecotropic retroviral transforming sequence c, CBLC	19q13.2	0.435
Hs.177534	dual specificity phosphatase 10, DUSP10	1q41	0.451
Hs.9914	follistatin, FST	5q11.2	0.456
Hs.351344	transmembrane protein 25, TMEM25	11q23.3	0.467
Hs.249441	WEE1 homolog (S. pombe), WEE1	11p15.3-p15.1	0.483
Hs.376206	Kruppel-like factor 4 (gut), KLF4	9q31	0.483

表2 正常肝组织、肝硬化组织和肝癌组织中GADD45β mRNA的表达

分组	阴性(n )	阳性(n )	阳性表达率(%)
正常肝组织	4	16	80
肝硬化组织	4	6	60a
肝癌组织	26	9	26b

χ² = 4.114,

^aP<0.05 vs 肝癌组织; χ² = 15.128,

^bP<0.01 vs 正常肝组织.

表3 正常肝组织、肝硬化组织和肝癌组织中GADD45β蛋白的表达

标本	n	阳性(%)	GADD45β表达强度
			-	+	++	+++
正常肝组织	20	17(85)	3	1	6	10
肝硬化组织	10	7(70)a	3	2	4	1
肝癌组织	35	11(31)b	24	7	3	1
合计	65	35	30	10	13	12

χ² = 4.821,

^aP<0.05, χ² = 14.615,

^bP<0.01 vs 肝癌组织; 三组间χ² = 24.619, P<0.01.

表4 GADD45β表达强度与肝癌组织分化及核分级的关系

分组	n	阳性(%)	Fisher's exact	GADD45β表达强度
				-	+	++	+++
肝癌组织	35	11(31.4)		24	7	3	2
分化程度
高分化	9	7(77.8)		2	3	3	1
中分化	14	2(14.3)b	0.004	12	2	0	0
低分化	12	2(16.7)b	0.008	10	2	0	0
Edmendsen分级
Ⅰ	8	7(87.5)		1	3	3	1
Ⅱ	9	2(22.2)a	0.018	7	2	0	0
Ⅲ	12	1(8.3)a	0.026	11	1	0	0
Ⅳ	6	1(17.0)	0.141	5	1	0	0

^aP<0.05 vs Ⅰ级;

^bP<0.01 vs 高分化组; χ² = 13.973, P<0.01肝癌分化程度间比较; χ² = 17.596, ^dP<0.01肝癌各核分级间比较.