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Zhi-Hong Peng, Jian-Min Yang, Sui-Hai Si, Dian-Chun Fang, Wen-Sheng Chen, Yuan-Hui Luo, Gastroenterology Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Supported by: the National Natural Science Foundation of China, No. 30070348.
Correspondence to: Jian-Min Yang, Gastroenterology Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. jianminyang@hotmail.com
Received: November 13, 2003 Revised: November 29, 2003 Accepted: December 8, 2003 Published online: May 15, 2004
AIM: To study the effects of metastasis-suppressor gene KAI1 on viscoelastic properties of hepatocellular carcinoma MHCC97-H cells with high metastatic potential.
METHODS: The viscoelastic properties of MHCC97-H cells with high metastatic potential transfected with sense or antisense KAI1 expression plasmid in our previous experiments were measured by means of micropipette aspiration technique.
RESULTS: The elastic coefficients K1, K2 and m of the MHCC97-H cells were significantly higher after transfected with sense KAI1 expression plasmid (P = 0.007), lower after transfected with antisense KAI1 expression plasmid (P = 0.000), and no significantly different after transfected with its control vector pCI-neo without KAI1 gene (P = 0.444), as compared with their paternal MHCC97-H cells.
CONCLUSION: The metastasis-suppressor gene KAI1 may significantly affect the viscoelastic properties of MHCC97-H cells with high metastatic potential. It offers an important clue to study the mechanisms of invasion and metastasis of the malignant tumor.
Key Words: N/A
Citation: Peng ZH, Yang JM, Si SH, Fang DC, Chen WS, Luo YH. Effects of metastasis-suppressor gene KAI1 on viscoelastic properties of hepatocellular carcinoma MHCC97-H cells with high metastatic potential. Shijie Huaren Xiaohua Zazhi 2004; 12(5): 1040-1043
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