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Screening of HCTP4 interacting proteins in leukocytes by yeast-two hybrid technique
Min Liu, Jun Cheng, Shu-Lin Zhang, Lin Wang, Qing Shao, Jian Zhang, Yao-Dong Liang,
Min Liu, Jun Cheng, Shu-Lin Zhang, Lin Wang, Qing Shao, Jian Zhang, Yao-Dong Liang, Gene Therapy Research Center, Institute of Infectious Diseases, The 302 Hospital of PLA, Beijing 100039, China
Supported by: Grants from the National Natural Scientific Foundation, No. C03011402, No. C30070689; and the 9.5 Research and Technique Foundation of PLA, No. 98D063; and the Launching Foundation for Student Studying Abroad of PLA, No. 98H038; and the 10.5 Youth Research and Technique Foundation of PLA, No. 01Q138; and the 10.5 Research and Technique Foundation of PLA, No. 01MB135.
Correspondence to: Dr. Jun Cheng, Gene Therapy Research Center, Institute of Infectious Diseases, The 302 Hospital of PLA, 100 Xisihuanzhong Road, Beijing 100039, China. cj@genetherapy.com.cn
Received: November 13, 2003 Revised: December 1, 2003 Accepted: December 16, 2003 Published online: April 15, 2004
AIM: To investigate the biological function of HCTP4, yeast-two hybrid was performed to screen proteins interacting with HCTP4 in leukocytes.
METHODS: The HCTP4 gene was amplified by polymerase chain reaction (PCR) and HCTP4 bait plasmid was constructed by using yeast-two hybrid system 3, then the constructed vector was transformed into yeast AH109. The transformed yeast mated with yeast Y187 containing leukocytes cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) and synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing x--gal for selecting two times and screening. After extracting and sequencing of plasmid from blue colonies, we underwent analysis by bioinformatics.
RESULTS: Forty-four colonies were sequenced, among which twenty-five colonies were immunoglobulin lambda light chain, six human DNA sequences from clone RP11-189K21, four human DNA sequences from clone RP11-507C10, two homo sapiens 12p BAC RPCI11-75L1, one homo sapiens BAC clone RP11-21M10, one homo sapiens ubiquitin ligase mind bomb (MIB), one homo sapiens genomic DNA, chromosome 11 clone: RP11-867O8, one human DNA sequence from clone RP3-509I19, one homo sapiens small nuclear ribonucleoprotein polypeptide G, one homo sapiens UMP-CMP kinase (UMP-CMPK), and a new gene.
CONCLUSION: Genes of HCTP4 interacting proteins in leukocytes are successfully cloned and the results bring some new clues for studying the biological functions of HCTP4 and associated proteins.
Key Words: N/A
Citation: Liu M, Cheng J, Zhang SL, Wang L, Shao Q, Zhang J, Liang YD. Screening of HCTP4 interacting proteins in leukocytes by yeast-two hybrid technique. Shijie Huaren Xiaohua Zazhi 2004; 12(4): 832-835
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