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Inhibitory effects of celecoxib on proliferation of human liver and gastric carcinoma cells in vitro
Jing Fan, Ke-Feng Dou, Kai-Zong Li
Jing Fan, Ke-Feng Dou, Kai-Zong Li, Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China
Correspondence to: Jing Fan, Department of Hepatobiliary Surgery,Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an 710032, Shanxi Province, China.firstname.lastname@example.org
Received: January 2, 2004 Revised: January 6, 2004 Accepted: January 12, 2004 Published online: March 15, 2004
AIM: To study the inhibitory effects of celecoxib on proliferation of human hepatoma SMMC-7721 cells and gastric cancer SGC-7901 cells in vitro.
METHODS: The two carcinoma cells were cultured with celecoxib at various concentrations(0, 20, 40, 80, 160 and 320 mmol/L). Growth suppression was detected with MTT colorimetric assay, cell apoptotic alterations were evaluated by transmission electron microscopy(TEM), and quantity of Cox-2 was evaluated by cytochemical staining.
RESULTS: The inhibition of proliferation on two carcinoma cells was observed(49.1% and 42.9% by 320 mmol/L celecoxib). The inhibitory effect was dose-dependent. Apoptotic cells were observed under transmission electron microscope. The different quantities of Cox-2 protein in cells were observed by cytochemical staining.
CONCLUSION: Celecoxib inhibits proliferation, induces apoptosis of human two carcinoma cells in vitro, and the effects have close relation to the quantities of Cox-2 protein in cells.
Key Words: N/A
Citation: Fan J, Dou KF, Li KZ. Inhibitory effects of celecoxib on proliferation of human liver and gastric carcinoma cells in vitro. Shijie Huaren Xiaohua Zazhi 2004; 12(3): 523-526
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