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IL-2单独或IL-2联合IL-4均可以诱导A-NK细胞, 但二者联合效果更好, 通过LDH-L测定活化的A-NK细胞可在体外杀伤K562, Anip973和CC95细胞(39.00±9.16 vs 77.68±12.80, 43.10±10.05 vs 80.02±13.74, 42.14±9.72 vs 79.10±2.65, P <0.01), 抑制人直肠癌肿瘤在裸鼠体内的生长(1.04±0.15 vs 0.62±0.16, P <0.01), 提示A-NK细胞膜表面存在IL-4受体的表达.
IL-4 enhances antitumor effect of IL-2 induced A-NK Cells on human colon carcinoma
Zhi-Hua Wang, Bao-Zhong Shen, Li Shi
Zhi-Hua Wang, Li Shi, Cancer Research Institute, Harbin Medical University, Harbin 150040, Heilongjiang Province, China
Bao-Zhong Shen, The Third College of Clinical Mediccine, Harbin Medical University, Harbin 150040, Heilongjiang Province, China
Supported by the National Natural Science Foundation of China, No. 39270765 and the Key Programs of Foundation of Heilongjiang Province, No.G00C190401
Correspondence to: Dr Zhi-Hua Wang, Cancer Research Institute, Harbin Medical University, 150 Haping Road, Harbin 150040, Heilongjiang Province, China. hljwzh@163.com
Received: December 10, 2002 Revised: December 15, 2002 Accepted: December 26, 2002 Published online: September 15, 2003
AIM
Using IL-4 combined with IL-2 to induce A-NK cells and to evaluate the cytotoxicity of A-NK cells and its inhibiting effect on colon tumor growth.
METHODS
The A-NK cells were activated with recombinant lymphokine IL-2 combined IL-4, the cytotoxicity of the effector cells was determined by LDH-L release assay. Its antitumor effect was investigated through growth inhibiting of human colon carcinoma cells in nude mice.
RESULTS
IL-2 alone or combined with IL-4 could induce the activity of A-NK cells successively which could kill K562, Anip973 and CC95 tumor cells in vitro by LDH-L release assay (39.00 ± 9.16 vs 77.68 ± 12.80, 43.10 ± 10.05 vs 80.02 ± 13.74, 42.14 ±9.72 vs 79.10 ±12.65, P <0.01) and inhibit the growth of human colon carcinoma cells in nude mice (1.04 ± 0.15 vs 0.62 ± 0.16, P <0.01).The results suggested that there was expression of IL-4 receptor on the surface of A-NK cells.
CONCLUSION
IL-4 can enhance the antitumor activity of IL-2 induced A-NK cells. The method may have some potential application value in human cancer treatment.
Key Words: N/A
Citation: Wang ZH, Shen BZ, Shi L. IL-4 enhances antitumor effect of IL-2 induced A-NK Cells on human colon carcinoma. Shijie Huaren Xiaohua Zazhi 2003; 11(9): 1375-1377
在抗肿瘤免疫中细胞免疫扮演一个重要的脚色. 近来很多针对A-NK细胞的研究提示与普通LAK细胞相比A-NK细胞有着更快的扩增速度, 更高的杀伤效果, 由此吸引了大批研究者的广泛关注[12-16]. Brunson et al [17]曾报告用荧光标记、IL-2活化的A-NK细胞过继转输后能选择性的聚集到建立的肺或肝转移灶内, 结合到肿瘤细胞和/或微小血管的内皮细胞上. Smits et al [18]指出由于大多数肿瘤细胞表面缺乏CD18的表达, A-NK细胞表面和CD18相关的整合素是溶解靶细胞所必须的.
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