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Effects of hepatocyte growth factor on fibrosis and hepatic expression of MMP-1 andTIMP-1
Liu-Lai Song, He-Sheng Luo, Bao-Ping Yu
Liu-Lai Song, He-Sheng Luo, Bao-Ping Yu, The Devision of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Correspondence to: He-Sheng Luo, The Devision of gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. firstname.lastname@example.org
Received: July 12, 2002 Revised: July 20, 2002 Accepted: July 29, 2002 Published online: February 15, 2003
AIM: To investigate the effect of hepatocyte growth factor (HGF) on severity of liver fibrosis and hepatic expressions of MMP-1, TIMP-1 and to explore the mechanism of HGF in preventing liver fibrosis in rats.
METHODS: Eighty male Wistar rats were randomly divided into normal control group (Group A, 16 rats), liver fibrosis model group (Group B, 54 rats) and HGF therapy group (Group C, 10 rats). The liver fibrosis model was induced by administration CCl4 intraperitoneally. Rats in Group C had been administered HGF for six weeks and were sacrificed afterwards. Eight rats from each of group A and B were randomly sacrificed on week 6 simultaneously as that in group C. The remaining rats in-group B were randomly further subdivided into liver fibrosis model group (Group D, 12 rats) and HGF therapy group (Group E, 10 rats), HGF was administered to rats in group E on week. 7.All rats in group D and E were sacrificed on week 10.Liver function and levels of serum hyaluronic acid (HA), mucin (LN), collegen type IV (CIV), procollagen III (PCIII) were tested; the expression of MMP-1 and TIMP-1 were determined by immunohistochemical staining and analyzed by computer.
RESULTS: Compared with Group B, the serum levels of ALT, AST, HA, LN, CIV, PCIII in Group C were significantly reduced (P < 0.01), MMP-1 activity was slightly increased (0.25 ± 0.02, vs 0.22 ± 0.05, P < 0.05), TIMP-1 activity was markedly reduced (0.34 ± 0.05, vs 0.45 ± 005, P < 0.01). TIMP-1 activity in Group E (0.31 ± 0.07) was also markedly reduced in comparison with Group D (0.42 ± 0.06) (P < 0.01).
CONCLUSIONS: HGF has obvious effect in preventing development of liver fibrosis; it might facilitate degradation of hepatic fibrosic tissue via increasing the MMP-1 activity and or inhibiting TIMP-1 activity.
Key Words: N/A
Citation: Song LL, Luo HS, Yu BP. Effects of hepatocyte growth factor on fibrosis and hepatic expression of MMP-1 andTIMP-1. Shijie Huaren Xiaohua Zazhi 2003; 11(2): 209-213
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