孙开来, 110001, 辽宁省沈阳市和平区北二马路92号, 中国医科大学医学遗传学教研室. sunkailai@21cn. com
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Dong-Mei Hao, Xiu-Ju Sun, Zhi-Hong Zheng, Guang He, Ming-Chao Ma, Kai-Lai Sun, Department of Medical Genetics, Chinese Medical University, Shenyang 110001, Liaoning Province, China
Hui-Mian Xu, Mei-Xian Wang, Department of Oncology, First Affiliated Hospital, Chinese Medical University, Shenyang 110001, Liaoning Province, China
Supported by: the National Major Basic Research Development Program, No. G1998051203.
Correspondence to: Dr. Kai-Lai Sun, Department of Medical Genetics, Chinese Medical University, No. 92, the 2th Northern Road, Heping District, Shenyang 110001, Liaoning Province, China. sunkailai@21cn. com
Received: November 12, 2002 Revised: November 25, 2002 Accepted: November 28, 2002 Published online: January 15, 2003
AIM: To explore molecular mechanism of gastric carcinogenesis, we screened associated genes of gastric dysplasia and further investigated their expression in gastric carcinomas with different stages.
METHODS: Relatively pure dysplasia and normal tissue were procured by manual microdissection, amplified by cDNA-PCR, and then used to carry out forward(dysplasia as tester, normal tissue as driver) and reverse(normal tissue as tester, dysplasia as driver) SSH. Subtracted cDNA fragments were cloned into vector, screened, sequenced, and made homologous analysis. The expression of differentially expressed fragments was detected and verified by Dot hybridization and reverse transcription-PCR.
RESULTS: Two subtracted cDNA libraries were constructed. Twenty-one of 26 sequenced clones were verified to be expressed differentially. It was noted that differentiall expressions of 4 genes(P125, cytochrome c oxidase subunit I, meprin A, acidic calponin)were detected simultaneously in dysplasia, early cancer and advanced cancer.
CONCLUSION: Four new associated genes have been identified in dysplasia. Further studies are necessary to determine their roles in gastric carcinogenesis.
Key Words: N/A
Citation: Hao DM, Sun XJ, Zheng ZH, He G, Ma MC, Xu HM, Wang MX, Sun KL. Screening and expression of associated genes in gastric dysplasia. Shijie Huaren Xiaohua Zazhi 2003; 11(1): 6-9
Li C, Liang Y, Wu M, Xu L, Cai W. Telomerase activity analysis of esophageal carcinoma using microdissection-TRAP assay.Chin Med J (Engl). 2002;115:1405-1408.
Li C, Wu M, Liang Y, Xu L, Cai W. [Analysis of telomerase activity in esophageal carcinoma using microdissection telomeric repeat amplification protocol assay].Zhonghua Yi Xue Za Zhi. 2002;82:39-42.
Kitahara O, Furukawa Y, Tanaka T, Kihara C, Ono K, Yanagawa R, Nita ME, Takagi T, Nakamura Y, Tsunoda T. Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays after laser-capture microdissection of tumor tissues and normal epithelia.Cancer Res. 2001;61:3544-3549.
Lottaz D, Maurer CA, Hahn D, Büchler MW, Sterchi EE. Nonpolarized secretion of human meprin alpha in colorectal cancer generates an increased proteolytic potential in the stroma.Cancer Res. 1999;59:1127-1133.