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Xiu-Ju Sun, Kai-Lai Sun, Hao Fu, Department of Molecular Genetics, China Medical University, Shenyang 110001, Liaoning Province, China
Shu-Bao Wang, Jun-Qing Chen, Department of Oncology, The First Clinical College, China Medical University, Shenyang 110001, Liaoning Province, China
Supported by: the National "973"Project(National key program on basic research)No. G19980501203.
Correspondence to: Kai-Lai Sun, Department of Molecular Genetics, China Medical University, Shenyang 110001, Liaoning Province, China. klsun@vip. sina. com
Received: June 1, 2002 Revised: June 25, 2002 Accepted: July 26, 2002 Published online: January 15, 2003
AIM: To explore the relationship between the expression of nm23H1 gene and the in vivo and in vitro. invasive capacity of gastric cancer cells
METHODS: The invasive capacity of gastric cancer cell in vitro was determined by Boyden chamber method. And the expressions of nm23H1 in gene and protein level were measured by Northern Blot, RT-PCR and immunohistochemical method in gastric cancer cell lines, respectively.
RESULTS: The order of the invasive capacity of gastric cancer cell lines were: MKN45, being the highest(33.1±5.23, P<0.01); BGC823(15.8±2.7) and MKN1(14.1±4.5), the moderate(there was no significant difference between them, P>0.05), and GT3TKB(6.3±2.5), the lowest(P<0.01). The expression of nm23H1 gene was negatively correlated with the in vivo invasive capacity of gastric cancer cell lines, and also negatively correlated with the in vitro invasive capacity of gastric cancer cell lines of MKN45, BGC823 and GT3TKB. But there was no relationship between the expression of nm23H1 gene and the invasive capacity of MKN1 in vitro.
CONCLUSION: The expression of nm23H1 gene is of great significance in evaluating the in vivo and in vitro invasive capacity of gastric cancer cell lines.
Key Words: N/A
Citation: Sun XJ, Sun KL, Fu H, Wang SB, Chen JQ. Relationship between expression of nm23H1 gene and in vivo and in vitro invasive capacity of gastric cancer cells. Shijie Huaren Xiaohua Zazhi 2003; 11(1): 10-13
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