转录因子ETS1 RNA干扰质粒的构建及其稳定转染胰腺癌细胞株的建立

图1 荧光显微镜观察ETS1干扰质粒转染胰腺癌PANC-1细胞后荧光表达情况(×200). A, B: shRNA 1组; C, D: shRNA 2组; E, F: shRNA 3组; A, C, E: 白光; B, D, F: 荧光.

图2 Western blot检测3条干扰质粒转染细胞后ETS1蛋白表达况. 1: con-shRNA组; 2: shRNA 1组; 3: shRNA 2组; 4: shRNA 3组. GAPDH: 内参.

图3 对照组及实验组经G418筛选3 wk后形成的阳性克隆在常规显微镜及荧光显微镜下观察结果(×200). A, B: shRNA 1 clone; C, D: con shRNA clone; A,C: 白光; B, D: 绿色荧光.

图4 Western blot检测对照质粒和干扰质粒稳转的细胞系中ETS1表达情况. 1: shRNA1 clone1; 2: control clone1; 3: shRNA1 clone2; 4: control clone2. GAPDH: 内参.