基础研究
Copyright ©The Author(s) 2005.
世界华人消化杂志. 2005-07-15; 13(13): 1558-1561
在线出版 2005-07-15. doi: 10.11569/wcjd.v13.i13.1558
图1
图1 总RNA提取.
图2
图2 Grx 基因的PCR扩增. 1-3: PCR product; 4: PCR Marker (1057, 770, 612, 495, 392, 341, 297, 210 bp fragment, from top to bottom).
图3
图3 重组质粒pRSET-Grx酶切鉴定. 1: PCR Marker; 2: pRSET-Grx digested with BamHⅠ+EcoRⅠ; 3: undigested pRSET-Grx; 4: undigested pRSETA; 5: pRSETA digested with BamHⅠ+EcoRⅠ; 6: plasmid Marker.
图4
图4 人脐静脉内皮细胞Grx编码区的cDNA序列.
图5
图5 150 g/L SDS-PAGE分析表达产物. 1: Bacterial sample before induced; 2: Bacterial sample after induced 4 h; 3: Bacterial sample after induced 5 h; 4: Low-range protein molecular weight markers.
引文著录: 张春晶, 周宏博, 邹朝霞, 董钦, 于海涛. 人谷氧还蛋白基因的分子克隆及表达. 世界华人消化杂志 2005; 13(13): 1558-1561