人幽门螺杆菌热休克蛋白A编码基因的克隆、表达及抗原性研究

图1 HspA编码基因PCR 产物的1%琼脂糖凝胶电脉分析. 1: DNA Marker; 2: Negative control; 3: PCR products of HspA gene.

图2 重组载体的PCR鉴定. 1: DNA marker; 2: PCR product with the template of pET32a(+)/HspA; 3: PCR product with the template of Top10/pET32a(+)/HspA; 4: Negative control.

图3 重组载体在BL21中表达的全菌蛋白的150g/L SDS-PAGE分析. 1, 2, 6-8: Expression of recombinant vector in BL21 after induction of 4h with IPTG; 3: Bacterial protein expressed in BL21 after induction of 4 h with IPTG; 4: Expression of pET32a(+)in BL21; 5: Standard protein marker(Mr 14.2; 20.1; 24; 29; 36; 45; 66X103).

图4 用western blot 方法检测表达产物的抗原性. 1. BL21/pET32a(+); 2. BL21/pET32a(+)/HspA.