Basic Research
Copyright ©The Author(s) 2003.
World J Gastroenterol. Jun 15, 2003; 9(6): 1347-1351
Published online Jun 15, 2003. doi: 10.3748/wjg.v9.i6.1347
Table 1 Strains and plasmids
Relevant characteristicsSource
Strains
S. flexneri 2a, 2457TWild type, NalrMaurelli AT
E. coli DH5αSupE44, ΔlacU169 (Φ80 lacZΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1, NalrOur lab
S. cerevisiae AH109MATa, trp1-901, leu2-3, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3::MEL1UAS-MEL1TATA-LacZClontech
Plasmids
pGBKT7GAL4 DNA-BD, TRP1, c-Myc epitope tag, Kmr Clontech
pGBKT-ipaCGAL4 DNA-BD fusion of IpaC, TRP1, c-Myc epitope tag, KmrThis study
pACT2GAL4 DNA-AD, HA epitope, LEU2, AprClontech
pLAM5'-1GAL4 DNA-BD fusion of the human lamin C, TRP1, a false-positive detection plasmid, AprClontech
p1A positive library plasmidThis study
Table 2 Detection of transcriptional activity of bait fusion protein in AH109
Tranformed plasmidsGrowth on SD His-X-Gal
pGBKT-ipaC-white
pGBKT-ipaC + pACT2-white
Table 3 Verification of the interaction in positive clones
Tranformed plasmidsβ-Gal activity
1p1white
2p1 + pGBKT7white
3p1 + pLAM5’-1white
4p1 + pGBKT-ipaCblue