Viral Hepatitis
Copyright ©The Author(s) 2003.
World J Gastroenterol. Nov 15, 2003; 9(11): 2490-2496
Published online Nov 15, 2003. doi: 10.3748/wjg.v9.i11.2490
Table 1 Comparison of the serum level of PDGF-BB, TGF-β1, TIMP-1, MMP-1 and TIMP-1/MMP-1 between patients and healthy blood donors (¯x±s)
Group (n)PDGF-BB (n g/L)TGF-β1 ( μg/L)TIMP-1 ( μg/L)MMP-1 ( μg/L)TIMP-1/ MMP-1
Patients67.75 ± 30.08b31.41 ± 23.22258.87 ± 77.75a6.05 ± 4.4483.66 ± 100.36b
Controls19.85 ± 10.2826.28 ± 16.69205.80 ± 35.667.98 ± 3.1331.95 ± 20.03
t11.4350.9122.646-1.793.176
P0.0000.3650.010.0770.002
Table 2 Comparison of the serum level of MMP-1 and TGF-β1 between patients at different fibrosis stages, inflammation grades, and with different severities of the disease and the healthy controls (¯x±s)
Group (n)MMP-1 ( μg/L)TGF-β1 ( μg/L)
Controls (20)7.98 ± 3.1326.28 ± 16.69
S2 (16)6.34 ± 2.9631.66 ± 18.01
S3 (31)6.80 ± 5.3434.19 ± 26.23
S4 (13)3.93 ± 2.93ac25.16 ± 20.90
G2 (2)7.66 ± 0.3618.25 ± 6.01
G3 (28)6.41 ± 4.9131.89 ± 21.61
G4 (30)5.61 ± 4.15a32.14 ± 25.25
Mild disease (28)6.56 ± 3.6634.39 ± 24.43
Severe disease (32)5.61 ± 5.0529.08 ± 21.97
Table 3 Comparison of TIMP-1mRNA, MMP-1mRNA and TIMP-1mRNA/MMP-1mRNA in PBMCs between patients and healthy controls (¯x±s)
Group (n)TIMP-1mRNAMMP-1mRNATIMP-1mRNA/ MMP-1mRNA
Patients (60)1.05 ± 0.69b0.39 ± 0.364.77 ± 3.78a
Controls (20)0.40 ± 0.190.24 ± 0.111.71 ± 0.89
t2.9340.7412.514
P0.0050.4620.016
Table 5 ROC curve analysis of nine indices
IndexAUCPCut-off pointSensitivity (%)Specificity (%)YI
PDGF-BB0.9850.000≥40.50 ng/L90.095.00.850
TIMP-1mRNA0.8760.000≥0.7973.71000.737
TIMP-1mRNA/MMP-1mRNA0.7920.005≥3.2065.81000.658
TIMP-1/MMP-10.7480.001≥34.6970.075.00.450
HA0.7280.003≥145.20 μg/L62.087.50.497
PCIII0.7270.004≥137.40 mg/L59.281.20.404
TIMP-10.7260.003≥254.00 μg/L46.795.00.417
CIV0.5830.287≥74.20 mg/L55.168.70.238
LN0.4630.636≥156.65 μg/L37.875.00.128
Table 6 Parameters of the combined diagnosis in the parallel test
Combined indicesSensitivity (%)Specificity (%)
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA + PDGF-BB99.095.0
PDGF-BB + TIMP-1mRNA + HA99.083.1
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA + PDGF-BB + HA99.683.1
PDGF-BB + TIMP-1mRNA/MMP-1mRNA + HA98.783.1
PDGF-BB + TIMP-1mRNA97.495.0
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA + HA97.087.5
PDGF-BB + TIMP-1mRNA/MMP-1mRNA96.695.0
PDGF-BB + HA96.083.1
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA91.0100
TIMP-1mRNA + HA90.087.5
TIMP-1mRNA/MMP-1mRNA + HA87.087.5
Table 7 Parameters of the combined diagnosis in the serial test
Combined indicesSensitivity (%)Specificity (%)
PDGF-BB + TIMP-1mRNA66.3100
PDGF-BB + TIMP-1mRNA/MMP-1mRNA59.2100
PDGF-BB + HA56.099.4
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA48.5100
TIMP-1mRNA + HA45.7100
PDGF-BB + TIMP-1mRNA + HA41.1100
TIMP-1mRNA/MMP-1mRNA + HA40.8100
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA + PDGF-BB40.4100
PDGF-BB + TIMP-1mRNA/MMP-1mRNA + HA36.7100
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA + HA30.1100
TIMP-1mRNA + TIMP-1mRNA/MMP-1mRNA + PDGF-BB + HA27.1100