Flow cytometric analysis of DNA, telomerase content and multi-gene expression in esophageal epithelial dysplasia

Table 1 Relationship between cellular DNA content and cytologic diagnosis

Cellular diagnosis	No.of cases	DI value (¯x ± s)	P value (t test)
Normal	217	1.01 ± 0.06	> 0.2
Mild dysplasia	306	1.03 ± 0.10	< 0.01
Moderte dysplasia	952	1.06 ± 0.27	< 0.01
Severe dysplasia	349	1.09 ± 0.14	< 0.01
Cancer	92	1.24 ± 0.18	< 0.01

Table 2 Relationship between DNA ploid and cytologic diagnosis

Cellular diagnosis	No.of cases	DNA	ploidy pattern	Rate ofheteroploid (%)	P value χ² test
		diploid	heteroploid
Normal	217	196	21	9.7	> 0.05
Mild dysplasia	306	280	26	8.5	> 0.01
Moderate dysplasia	952	791	161	16.9	< 0.01
Severe dysplasia	349	229	120	34.4	< 0.01
Cancer	92	20	72	78.3	< 0.01

Table 3 Expression of P53, P21 in various lesions of esophageal epithelial cells

Cytological group	No.of cases	P53 FI (¯x ± s)	P53+ cases (%)	P21 FI (¯x ± s)	P21+ cases (%)
Normal	10	1.00 ± 0.11	0	1.00 ± 0.19	0
Mild dysplasia	24	1.10 ± 0.15	5 (20.8%)	1.09 ± 0.21	6 (25.0%)
Severe dysplasia	61	1.36 ± 0.32	34 (54.8%)	1.34 ± 0.35	35 (57.4%)
Cancer	5	2.28 ± 0.20	5 (100%)	1.82 ± 0.24	5 (100%)

Table 4 Results of cyclin D1 and TP1 expression in various lesions of esophageal epithelia

Cytologic diagnosis	No.of cases	Cyclin D1, FI (¯x ± s)	Cyclin D1+ cases	TP FI (¯x ± s)	TP1+ cases
Normal	7	1.00 ± 0.07	0	0.99 ± 0.07	0
Mild dysplasia	11	0.97 ± 0.12	1 (9.1%)	1.22 ± 0.15	7 (63.6%)
Severe dysplasia	76	1.21 ± 0.22	45 (59.2%)	1.33 ± 0.27	64 (84.2%)
Cancer	6	1.84 ± 0.18	6 (100%)	1.70 ± 0.15	6 (100%)