Effects of cold preservation and warm reperfusion on rat fatty liver

Table 1 Components of choline-deficient diet (CDD)

Components	Percentage
Casein	8.0
Lard	47.32
Sucrose	39.275
Minerals	4.0
Vitamins	0.65
L-cystine	0.625
Mg (OH)2MgCO3	0.1
Vitamin D3	0.02
Vitamin E	0.01
Total	100.0

Table 2 Portal perfusion pressure, ET-1 and bile production (mean ± SD, n = 7)

Group	Preservation time (hours)	Portal perfusion pressure (cmH2O)	ET-1 (ng/L)	Bile secretory volume (μL·min¯¹·g¯¹·wt)
Control	0	9.5 ± 0.8	35.6 ± 5.8	0.40 ± 0.09
	6	12.8 ± 1.3	60.2 ± 8.4	0.28 ± 0.07
	12	16.0 ± 1.7	83.7 ± 11.9	0.15 ± 0.05
Mildly fatty	12	16.5 ± 2.1	85.9 ± 13.4	0.16 ± 0.05
Moderately fatty	6	13.9 ± 1.7	65.8 ± 10.1	0.26 ± 0.08
	12	20.1 ± 2.3b	124.5 ± 27.6b	0.11 ± 0.03a
Severely fatty	0	9.8 ± 1.0	32.7 ± 4.9	0.38 ± 0.08
	6	18.5 ± 2.1c	90.4 ± 15.9c	0.12 ± 0.03c

^aP ＜ 0.05 vs control (12 h);

^bP ＜ 0.01 vs control (12 h);

^cP ＜ 0.01 vs control (6 h).

Table 3 AST, ALT, LDH in the effluent and mortality of SLC (mean ± SD, n = 7)

Group	Preservation time (hours)	AST (u•L¯¹•g¯¹•wt)	ALT (u•L¯¹•g¯¹•wt)	LDH (u•L¯¹•g¯¹•wt)	Mortality of SLE (%)
Control	0	2.42 ± 0.08	8.75 ± 1.40	23.64 ± 4.52	<0.5
	6	2.58 ± 0.12	8.90 ± 1.51	25.72 ± 4.79	5.9 ± 1.4
	12	9.65 ± 1.82	16.75 ± 3.10	54.74 ± 9.70	13.8 ± 2.8
Mildly fatty	12	10.20 ± 2.10	18.20 ± 4.20	58.26 ± 12.15	15.1 ± 3.2
Moderately fatty	6	2.63 ± 0.21	9.01 ± 1.72	28.40 ± 5.10	7.0 ± 1.8
	12	13.50 ± 2.74b	24.10 ± 5.86b	74.48 ± 19.5 3b	18.7 ± 4.3a
Severely fatty	0	2.10 ± 0.06	8.60 ± 1.25	21.70 ± 4.30	<0.5
	6	11.82 ± 1.97c	19.45 ± 4.76c	62.75 ± 17.54c	17.4 ± 3.5c

^aP ＜ 0.05 vs control (12 h);

^bP ＜ 0.01 vs control (12 h);

^cP ＜ 0.01 vs control (6 h).