Estrogen augmented visceral pain and colonic neuron modulation in a double-hit model of prenatal and adult stress

Table 1 Electrophysiological characteristics of colon related DRG neuron

Classification	Ctr., n = 36	CAS, n = 34	CPS, n = 29	CAS + CPS, n = 45
Spontaneous spike number	7.4 ± 4.0	16.9 ± 6.6	11.7 ± 4.8	44.8 ± 14.6c
Membrane capacitance (pF)	72.1 ± 4.9	95.4 ± 5.8	85.1 ± 6.2	93.8 ± 8.9a
Action potential threshold (mV)	-27.1 ± 2.5	-29.2 ± 1.8	-34.6 ± 1.6	-38.6 ± 1.4c
Resting membrane potential (mV)	-60.1 ± 1.7	-50.1 ± 1.3	-53.8 ± 1.5	-56.4 ± 1.3
Cell diameter (μm)	32 ± 0.9	29 ± 0.6	31 ± 0.8	31 ± 0.6
Time constant (μm)	545.5 ± 51.1	737.7 ± 70.4	595.9 ± 54.1	535.3 ± 42.5
Action potential amplitude (mV)	79.0 ± 4.7	80.2 ± 3.9	77.4 ± 4.2	85.6 ± 3.7
Duration (ms)	8.38 ± 0.97	11.2 ± 0.95	12.3 ± 1.83	8.89 ± 0.60

Values means ± standard error.

^aP < 0.05;

^cP < 0.001 vs control group. CAS: Chronic adult stress; CPS: Chronic prenatal stress.

Table 2 Electrophysiological characteristics of colon related DRG neuron after Letrozole treatment

Classification	Veh. + CAS + CPS, n = 60	Let + CAS + CPS, n = 27
Spontaneous spike number	34.6 ± 11.3	8.04 ± 4.62c
Membrane capacitance (pF)	86.8 ± 7.1	83.7 ± 6.7
Action potential threshold (mV)	-34.6 ± 1.6	-27.6 ± 2.1a
Resting membrane potential (mV)	-54.9 ± 1.3	-54.6 ± 2.2
Cell diameter (μm)	31 ± 0.5	27 ± 1.6
Time constant (um)	502.7 ± 34.5	454.0 ± 36.6
Action potential amplitude (mV)	90.9 ± 3.7	89.8 ± 7.2
Duration (ms)	8.67 ± 0.63	10.70 ± 2.22

Values are means ± standard error.

^aP < 0.05;

^cP < 0.001 vs control group. CAS: Chronic adult stress; CPS: Chronic prenatal stress.