Basic Study
Copyright ©The Author(s) 2020.
World J Gastroenterol. Aug 28, 2020; 26(32): 4763-4785
Published online Aug 28, 2020. doi: 10.3748/wjg.v26.i32.4763
Table 1 Primers used for transcription analysis
GeneForward primer (5’-3’)Reverse primer (5’-3’)
Actb (β-actin)TCACCCACACTGTGCCCATCTACGCAGCGGAACCGCTCATTGCCAATGG
Il17aTCAGCGTGTCCAAACACTGAGCGCCAAGGGAGTAAAGACTT
Foxp3AGCAGTCCACTTCACCAAGGGGATAACGCCAGAGGAGCTG
RorcCCGCTGAGAGGGCTTCACTGCAGGAGTAGGCCACATTACA
Il10TGAATTCCCTGGGTGAGAAGCATCACTCTTCACCTCCAC
Il6AGCCAGAGTCCTTCAGAGAGATAAAAAGTGCCGCTACCCTGA
Table 2 Colitis symptom score P values
Treatment groups2% WeightStool bloodDiarrheaDAI
Ctrl vsHm0.24950.614210.024810.06591
Hm/DSS< 0.00011< 0.00011< 0.00011< 0.00011
Hm/DSS/I3C0.9574< 0.00011< 0.00011< 0.00011
DSS0.0552< 0.00011< 0.00011< 0.00011
DSS/I3C0.1381< 0.00011< 0.00011< 0.00011
Hm vsDSS0.9823< 0.00011< 0.00011< 0.00011
Hm/DSS< 0.00011< 0.00011< 0.00011< 0.00011
Hm/DSS vsDSS< 0.000110.000710.00411< 0.00011
Hm/DSS/I3C< 0.00011< 0.00011< 0.00011< 0.00011
DSS vsDSS/I3C0.99770.00261< 0.00011< 0.00011
Table 3 MicroRNA expression
Raw expression data
Fold changes
miRNACtrlHmHm/DSSDSS/Hm/ I3CDSSDSS/ I3CHm vs CtrlHm/DSS vs CtrlDSS/Hm/I3C vs CtrlDSS vs CtrlDI vs CtrlDMI vsDHMDI vs DSS
mmu-let-7a-5p12.9311.145.729.1110.7110.59-3.45-147.58-14.06-4.65-5.0710.5-1.09
mmu-let-7b-5p14.1411.847.9710.4511.2110.89-4.92-72.01-12.96-7.62-9.565.56-1.25
mmu-let-7d-5p13.7611.528.0910.4411.110.86-4.71-50.67-9.97-6.29-7.425.08-1.18
mmu-let-7e-5p11.0610.061.78.399.69.23-2-654.64-6.34-2.74-3.54103.31-1.29
mmu-let-7g-5p10.316.711.372.017.526.16-12.19-491.93-317.1-6.92-17.811.55-2.58
mmu-miR-103-3p11.8510.446.4210.0910.8810.02-2.66-43.07-3.39-1.96-3.5712.7-1.82
mmu-miR-106a-5p9.295.451.152.035.512.78-14.34-282.96-153.91-13.78-91.541.84-6.64
mmu-miR-127-3p7.94.550.952.82.431.91-10.18-123.98-34.2-44.34-63.553.63-1.43
mmu-miR-128-3p5.810.790.941.040.771.3-32.4-29.23-27.31-33.04-22.871.071.44
mmu-miR-135a-1-3p3.641.31.81.261.432.12-5.04-3.57-5.19-4.62-2.86-1.451.62
mmu-miR-140-3p10.29.921.837.919.628.3-1.21-329.16-4.87-1.49-3.7367.62-2.51
mmu-miR-140-5p6.331.380.791.021.380.87-30.86-46.56-39.48-30.86-43.841.18-1.42
mmu-miR-142-5p2.90.991.350.991.161.01-3.78-2.93-3.78-3.34-3.72-1.29-1.11
mmu-miR-145a-5p12.9911.516.7910.211.1510.28-2.78-73.5-6.89-3.57-6.5510.66-1.83
mmu-miR-146a-5p10.347.841.243.547.727.84-5.65-550.48-111.6-6.13-22.74.93-3.7
mmu-miR-150-5p13.411.14.148.9610.49.76-4.92-614.48-21.78-8.01-12.5128.22-1.56
mmu-miR-155-5p9.868.151.543.587.655.31-3.27-320.03-77.72-4.61-23.484.12-5.09
mmu-miR-15b-5p11.1710.171.4889.928.84-1.99-824.5-9.01-2.37-5.0291.56-2.12
mmu-miR-16-5p13.1810.381.359.110.89.11-7-3646.25-16.92-5.22-16.9215.51-3.23
mmu-miR-17-5p10.719.011.337.89.297.82-3.25-666.3-7.53-2.67-7.4388.54-2.78
mmu-miR-185-5p9.276.580.995.645.865.07-6.45-310.71-12.33-10.58-18.3625.19-1.74
mmu-miR-18a-5p7.321.370.841.371.411.74-61.84-89.41-61.84-60.29-47.911.451.26
mmu-miR-195a-5p10.598.031.915.038.227.39-5.89-409.38-47.19-5.17-9.148.68-1.77
mmu-miR-196b-5p3.110.940.941.081.050.83-4.49-4.49-4.09-4.19-4.861.1-1.16
mmu-miR-199a-5p9.54.561.772.017.721.77-2.03-101.88-21.49-3.71-78.214.74-21.08
mmu-miR-19a-3p4.081.561.390.911.241.25-5.73-6.45-9.02-7.15-7.13-1.41
mmu-miR-19b-3p9.436.131.185.877.975.61-9.84-303.99-11.79-2.74-14.0725.78-5.14
mmu-miR-20b-5p9.034.871.762.295.851.96-17.77-154.33-106.75-9.05-133.961.45-14.8
mmu-miR-221-3p9.196.071.241.427.455.25-8.65-246.56-218.06-3.34-15.341.13-4.59
mmu-miR-222-3p8.897.651.124.116.244.58-2.36-219.12-27.5-6.29-19.867.97-3.16
mmu-miR-223-3p5.451.571.010.991.840.87-14.69-21.58-21.99-12.14-23.85-1.02-1.96
mmu-miR-24-2-5p12.7410.841.789.5110.810.12-56.17-101.73-64.83-72.38-48.351.571.5
mmu-miR-27a-3p9.965.941.351.578.225.2-16.28-390.62-337.26-3.34-27.111.16-8.12
mmu-miR-28a-3p7.534.750.831.41.491.12-6.86-103.85-69.7-65.83-84.81.49-1.29
mmu-miR-29a-3p10.978.842.387.739.457.38-4.37-386.19-9.45-2.86-12.0640.85-4.21
mmu-miR-29c-3p5.380.871.231.282.051.8-22.8-17.81-17.22-10.08-12.011.03-1.19
mmu-miR-30e-5p7.81.131.641.643.461.32-101.76-71.46-71.46-14.68-89.041-4.42
mmu-miR-345-5p5.412.462.853.652.811.18-7.76-5.93-3.41-6.1-18.841.74-3.09
mmu-miR-374b-5p4.340.921.190.891.581.13-10.7-8.87-10.91-6.78-9.23-1.23-1.36
mmu-miR-423-5p7.073.131.294.252.561.26-15.33-54.69-7.03-22.66-55.787.78-2.46
mmu-miR-491-5p2.951.391.151.011.341.07-2.96-3.49-3.84-3.06-3.7-1.1-1.21
mmu-miR-532-5p8.45.550.893.433.451.75-7.22-183.34-31.45-30.94-101.045.83-3.27
mmu-miR-760-3p2.440.990.741.11.151.17-2.73-3.25-2.54-2.46-2.421.281.02
mmu-miR-877-5p4.053.561.932.143.281.8-1.4-4.35-3.75-1.7-4.761.16-2.79
mmu-miR-93-5p10.418.671.867.648.666.85-3.34-373.43-6.79-3.35-11.7754.98-3.52